Vibrio tapetis Displays an Original Type IV Secretion System in Strains Pathogenic for Bivalve Molluscs
The Brown Ring Disease (BRD) caused high mortality rates since 1986 in the Manila clam Venerupis philippinarum introduced and cultured in Western Europe from the 1970s. The causative agent of BRD is a Gram-Negative bacterium, Vibrio tapetis, which is also pathogenic to fish. Here we report the first...
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Format: | Article |
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Frontiers Media S.A.
2018-02-01
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Series: | Frontiers in Microbiology |
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Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00227/full |
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doaj-da38075d8206475c83ba3c7948862973 |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Graciela M. Dias Graciela M. Dias Adeline Bidault Patrick Le Chevalier Gwenaëlle Choquet Clio Der Sarkissian Ludovic Orlando Ludovic Orlando Claudine Medigue Valerie Barbe Sophie Mangenot Cristiane C. Thompson Fabiano L. Thompson Annick Jacq Vianney Pichereau Christine Paillard |
spellingShingle |
Graciela M. Dias Graciela M. Dias Adeline Bidault Patrick Le Chevalier Gwenaëlle Choquet Clio Der Sarkissian Ludovic Orlando Ludovic Orlando Claudine Medigue Valerie Barbe Sophie Mangenot Cristiane C. Thompson Fabiano L. Thompson Annick Jacq Vianney Pichereau Christine Paillard Vibrio tapetis Displays an Original Type IV Secretion System in Strains Pathogenic for Bivalve Molluscs Frontiers in Microbiology Vibrio tapetis Venerupis philippinarum comparative genomics pathogenicity T4SS pangenome |
author_facet |
Graciela M. Dias Graciela M. Dias Adeline Bidault Patrick Le Chevalier Gwenaëlle Choquet Clio Der Sarkissian Ludovic Orlando Ludovic Orlando Claudine Medigue Valerie Barbe Sophie Mangenot Cristiane C. Thompson Fabiano L. Thompson Annick Jacq Vianney Pichereau Christine Paillard |
author_sort |
Graciela M. Dias |
title |
Vibrio tapetis Displays an Original Type IV Secretion System in Strains Pathogenic for Bivalve Molluscs |
title_short |
Vibrio tapetis Displays an Original Type IV Secretion System in Strains Pathogenic for Bivalve Molluscs |
title_full |
Vibrio tapetis Displays an Original Type IV Secretion System in Strains Pathogenic for Bivalve Molluscs |
title_fullStr |
Vibrio tapetis Displays an Original Type IV Secretion System in Strains Pathogenic for Bivalve Molluscs |
title_full_unstemmed |
Vibrio tapetis Displays an Original Type IV Secretion System in Strains Pathogenic for Bivalve Molluscs |
title_sort |
vibrio tapetis displays an original type iv secretion system in strains pathogenic for bivalve molluscs |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-02-01 |
description |
The Brown Ring Disease (BRD) caused high mortality rates since 1986 in the Manila clam Venerupis philippinarum introduced and cultured in Western Europe from the 1970s. The causative agent of BRD is a Gram-Negative bacterium, Vibrio tapetis, which is also pathogenic to fish. Here we report the first assembly of the complete genome of V. tapetis CECT4600T, together with the genome sequences of 16 additional strains isolated across a broad host and geographic range. Our extensive genome dataset allowed us to describe the pathogen pan- and core genomes and to identify putative virulence factors. The V. tapetis core genome consists of 3,352 genes, including multiple potential virulence factors represented by haemolysins, transcriptional regulators, Type I restriction modification system, GGDEF domain proteins, several conjugative plasmids, and a Type IV secretion system. Future research on the coevolutionary arms race between V. tapetis virulence factors and host resistance mechanisms will improve our understanding of how pathogenicity develops in this emerging pathogen. |
topic |
Vibrio tapetis Venerupis philippinarum comparative genomics pathogenicity T4SS pangenome |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2018.00227/full |
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doaj-da38075d8206475c83ba3c79488629732020-11-24T23:18:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-02-01910.3389/fmicb.2018.00227323112Vibrio tapetis Displays an Original Type IV Secretion System in Strains Pathogenic for Bivalve MolluscsGraciela M. Dias0Graciela M. Dias1Adeline Bidault2Patrick Le Chevalier3Gwenaëlle Choquet4Clio Der Sarkissian5Ludovic Orlando6Ludovic Orlando7Claudine Medigue8Valerie Barbe9Sophie Mangenot10Cristiane C. Thompson11Fabiano L. Thompson12Annick Jacq13Vianney Pichereau14Christine Paillard15Laboratoire des Sciences de l'Environnement Marin, Université de Bretagne Occidentale, UMR 6539 UBO/Centre National de la Recherche Scientifique/IRD/Ifremer, Institut Universitaire Européen de la Mer, Plouzané, FranceLaboratório de Microbiologia, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratoire des Sciences de l'Environnement Marin, Université de Bretagne Occidentale, UMR 6539 UBO/Centre National de la Recherche Scientifique/IRD/Ifremer, Institut Universitaire Européen de la Mer, Plouzané, FranceLaboratoire de Biotechnologie et Chimie Marine, Université de Bretagne Occidentale, Quimper, FranceLaboratoire des Sciences de l'Environnement Marin, Université de Bretagne Occidentale, UMR 6539 UBO/Centre National de la Recherche Scientifique/IRD/Ifremer, Institut Universitaire Européen de la Mer, Plouzané, FranceCentre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Copenhagen, DenmarkCentre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Copenhagen, DenmarkLaboratoire d'Anthropobiologie Moléculaire et d'Imagerie de Synthèse, Centre National de la Recherche Scientifique UMR 5288, Université de Toulouse, Université Paul Sabatier, Toulouse, FranceCEA, Genoscope, Laboratoire d'Analyses Bioinformatiques pour la Génomique et le Métabolisme, Université d'Evry, Centre National de la Recherche Scientifique-UMR 8030, Evry, FranceCEA, Genoscope, Laboratoire d'Analyses Bioinformatiques pour la Génomique et le Métabolisme, Université d'Evry, Centre National de la Recherche Scientifique-UMR 8030, Evry, FranceCEA, Institut de biologie François-Jacob, Genoscope, Laboratoire de Biologie Moléculaire pour l'Etude des Génomes, Evry, FranceLaboratório de Microbiologia, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Microbiologia, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstitute for Integrative Biology of the Cell, CEA, Centre National de la Recherche Scientifique, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, FranceLaboratoire des Sciences de l'Environnement Marin, Université de Bretagne Occidentale, UMR 6539 UBO/Centre National de la Recherche Scientifique/IRD/Ifremer, Institut Universitaire Européen de la Mer, Plouzané, FranceLaboratoire des Sciences de l'Environnement Marin, Université de Bretagne Occidentale, UMR 6539 UBO/Centre National de la Recherche Scientifique/IRD/Ifremer, Institut Universitaire Européen de la Mer, Plouzané, FranceThe Brown Ring Disease (BRD) caused high mortality rates since 1986 in the Manila clam Venerupis philippinarum introduced and cultured in Western Europe from the 1970s. The causative agent of BRD is a Gram-Negative bacterium, Vibrio tapetis, which is also pathogenic to fish. Here we report the first assembly of the complete genome of V. tapetis CECT4600T, together with the genome sequences of 16 additional strains isolated across a broad host and geographic range. Our extensive genome dataset allowed us to describe the pathogen pan- and core genomes and to identify putative virulence factors. The V. tapetis core genome consists of 3,352 genes, including multiple potential virulence factors represented by haemolysins, transcriptional regulators, Type I restriction modification system, GGDEF domain proteins, several conjugative plasmids, and a Type IV secretion system. Future research on the coevolutionary arms race between V. tapetis virulence factors and host resistance mechanisms will improve our understanding of how pathogenicity develops in this emerging pathogen.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00227/fullVibrio tapetisVenerupis philippinarumcomparative genomicspathogenicityT4SSpangenome |