The Effect of Shaker Use on Embryo Yield in Shed-Microspore Cultures of Ornamental Peppers

• Main Authors: Esin Arı, Selcen Doğan
• Format: Article
• Language: English
• Published: Abant İzzet Baysal University 2019-12-01
• Series: Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi
• Subjects: capsicum annuum l.; doubled haploid; microspore embryogenesis; culture shaking; anter; mikrospor embriyogenesis; çalkalama
• Online Access: https://dergipark.org.tr/tr/pub/ijaws/issue/50517/627679

Because of their morphological diversity and various rich content, ornamental peppers (Capsicum annuum L.) has become a favored plant in ornamental plant, food, cosmetics and medicinal sectors in recent years. This interest increased the ornamental pepper breeding studies. However, there are limited numbers of study based on haploid technology in ornamental peppers. In this study, we aimed to determine the effect of using shaker on embryo yield in the shed-microspore cultures of ornamental peppers. We used a total of 29 genotypes and compared the shaken and stationary cultures for each genotype. The shaken cultures gave more successful androgenic performance in terms of total and globular embryo formations. The most successful embryogenic performance was obtained from Genotype 754 in both stationary and shaken cultures. However, the shaken cultures of Genotype 754 formed 4.5 times more embryos when compared to its stationary control group. As the result, the use of shaker positively affected embryo yield in shed-microspore cultures of ornamental pepper depending on genotype.