Inhibition of Oxidative Stress by Low-Molecular-Weight Polysaccharides with Various Functional Groups in Skin Fibroblasts
The aim of this study was to evaluate the in cellulo inhibition of hydrogen-peroxide-induced oxidative stress in skin fibroblasts using different low-molecular-weight polysaccharides (LMPS) prepared from agar (LMAG), chitosan (LMCH) and starch (LMST), which contain various different functional group...
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Online Access: | http://www.mdpi.com/1422-0067/14/10/19399 |
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doaj-da3f6859db624679b0d04dcc04c2a52b2020-11-25T00:25:33ZengMDPI AGInternational Journal of Molecular Sciences1422-00672013-09-011410193991941510.3390/ijms141019399Inhibition of Oxidative Stress by Low-Molecular-Weight Polysaccharides with Various Functional Groups in Skin FibroblastsGregor P. C. DrummenRong-Huei ChenMin-Lang TsaiSzu-Kai ChenChu-Hsi HsuThe aim of this study was to evaluate the in cellulo inhibition of hydrogen-peroxide-induced oxidative stress in skin fibroblasts using different low-molecular-weight polysaccharides (LMPS) prepared from agar (LMAG), chitosan (LMCH) and starch (LMST), which contain various different functional groups (i.e., sulfate, amine, and hydroxyl groups). The following parameters were evaluated: cell viability, intracellular oxidant production, lipid peroxidation, and DNA damage. Trolox was used as a positive control in order to allow comparison of the antioxidant efficacies of the various LMPS. The experimentally determined attenuation of oxidative stress by LMPS in skin fibroblasts was: LMCH > LMAG > LMST. The different protection levels of these LMPS may be due to the physic-chemical properties of the LMPS’ functional groups, including electron transfer ability, metal ion chelating capacities, radical stabilizing capacity, and the hydrophobicity of the constituent sugars. The results suggest that LMCH might constitute a novel and potential dermal therapeutic and sun-protective agent.http://www.mdpi.com/1422-0067/14/10/19399antioxidantskin fibroblastoxidative damageoxidative stressROSlipid peroxidationDNA damageagarchitosanstarch |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gregor P. C. Drummen Rong-Huei Chen Min-Lang Tsai Szu-Kai Chen Chu-Hsi Hsu |
spellingShingle |
Gregor P. C. Drummen Rong-Huei Chen Min-Lang Tsai Szu-Kai Chen Chu-Hsi Hsu Inhibition of Oxidative Stress by Low-Molecular-Weight Polysaccharides with Various Functional Groups in Skin Fibroblasts International Journal of Molecular Sciences antioxidant skin fibroblast oxidative damage oxidative stress ROS lipid peroxidation DNA damage agar chitosan starch |
author_facet |
Gregor P. C. Drummen Rong-Huei Chen Min-Lang Tsai Szu-Kai Chen Chu-Hsi Hsu |
author_sort |
Gregor P. C. Drummen |
title |
Inhibition of Oxidative Stress by Low-Molecular-Weight Polysaccharides with Various Functional Groups in Skin Fibroblasts |
title_short |
Inhibition of Oxidative Stress by Low-Molecular-Weight Polysaccharides with Various Functional Groups in Skin Fibroblasts |
title_full |
Inhibition of Oxidative Stress by Low-Molecular-Weight Polysaccharides with Various Functional Groups in Skin Fibroblasts |
title_fullStr |
Inhibition of Oxidative Stress by Low-Molecular-Weight Polysaccharides with Various Functional Groups in Skin Fibroblasts |
title_full_unstemmed |
Inhibition of Oxidative Stress by Low-Molecular-Weight Polysaccharides with Various Functional Groups in Skin Fibroblasts |
title_sort |
inhibition of oxidative stress by low-molecular-weight polysaccharides with various functional groups in skin fibroblasts |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2013-09-01 |
description |
The aim of this study was to evaluate the in cellulo inhibition of hydrogen-peroxide-induced oxidative stress in skin fibroblasts using different low-molecular-weight polysaccharides (LMPS) prepared from agar (LMAG), chitosan (LMCH) and starch (LMST), which contain various different functional groups (i.e., sulfate, amine, and hydroxyl groups). The following parameters were evaluated: cell viability, intracellular oxidant production, lipid peroxidation, and DNA damage. Trolox was used as a positive control in order to allow comparison of the antioxidant efficacies of the various LMPS. The experimentally determined attenuation of oxidative stress by LMPS in skin fibroblasts was: LMCH > LMAG > LMST. The different protection levels of these LMPS may be due to the physic-chemical properties of the LMPS’ functional groups, including electron transfer ability, metal ion chelating capacities, radical stabilizing capacity, and the hydrophobicity of the constituent sugars. The results suggest that LMCH might constitute a novel and potential dermal therapeutic and sun-protective agent. |
topic |
antioxidant skin fibroblast oxidative damage oxidative stress ROS lipid peroxidation DNA damage agar chitosan starch |
url |
http://www.mdpi.com/1422-0067/14/10/19399 |
work_keys_str_mv |
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