In Vivo Applications of Single Chain Fv (Variable Domain) (scFv) Fragments
Single chain variable domain (Fv) fragments (scFv) are powerful tools in research and clinical settings, owing to better pharmacokinetic properties compared to the parent monoclonal antibodies and the relative ease of producing them in large quantities, at low cost. Though they offer several advanta...
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Online Access: | http://www.mdpi.com/2073-4468/2/2/193 |
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doaj-da430719285c4582a92c2120f13f4c1f2020-11-25T00:05:41ZengMDPI AGAntibodies2073-44682013-04-012219320810.3390/antib2020193In Vivo Applications of Single Chain Fv (Variable Domain) (scFv) FragmentsNardos G. TassewPhilippe P. MonnierRobin J. VigourouxSingle chain variable domain (Fv) fragments (scFv) are powerful tools in research and clinical settings, owing to better pharmacokinetic properties compared to the parent monoclonal antibodies and the relative ease of producing them in large quantities, at low cost. Though they offer several advantages, they suffer from lower binding affinity and rapid clearance from circulation, which limits their therapeutic potential. However, these fragments can be genetically modified to enhance desirable properties, such as multivalency, high target retention and slower blood clearance, and as such, a variety of scFv formats have been generated. ScFvs can be administered by systemic injection for diagnostic and therapeutic purposes. They can be expressed in vivo through viral vectors in instances where large infection rates and sustenance of high levels of the antibody is required. ScFvs have found applications as tools for in vivo loss-of-function studies and inactivation of specific protein domains, diagnostic imaging, tumor therapy and treatment for neurodegenerative and infectious diseases. This review will focus on their in vivo applications.http://www.mdpi.com/2073-4468/2/2/193scFvrecombinant antibodiesin vivo expressionadeno-associated virus (AAV)immunotherapy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nardos G. Tassew Philippe P. Monnier Robin J. Vigouroux |
spellingShingle |
Nardos G. Tassew Philippe P. Monnier Robin J. Vigouroux In Vivo Applications of Single Chain Fv (Variable Domain) (scFv) Fragments Antibodies scFv recombinant antibodies in vivo expression adeno-associated virus (AAV) immunotherapy |
author_facet |
Nardos G. Tassew Philippe P. Monnier Robin J. Vigouroux |
author_sort |
Nardos G. Tassew |
title |
In Vivo Applications of Single Chain Fv (Variable Domain) (scFv) Fragments |
title_short |
In Vivo Applications of Single Chain Fv (Variable Domain) (scFv) Fragments |
title_full |
In Vivo Applications of Single Chain Fv (Variable Domain) (scFv) Fragments |
title_fullStr |
In Vivo Applications of Single Chain Fv (Variable Domain) (scFv) Fragments |
title_full_unstemmed |
In Vivo Applications of Single Chain Fv (Variable Domain) (scFv) Fragments |
title_sort |
in vivo applications of single chain fv (variable domain) (scfv) fragments |
publisher |
MDPI AG |
series |
Antibodies |
issn |
2073-4468 |
publishDate |
2013-04-01 |
description |
Single chain variable domain (Fv) fragments (scFv) are powerful tools in research and clinical settings, owing to better pharmacokinetic properties compared to the parent monoclonal antibodies and the relative ease of producing them in large quantities, at low cost. Though they offer several advantages, they suffer from lower binding affinity and rapid clearance from circulation, which limits their therapeutic potential. However, these fragments can be genetically modified to enhance desirable properties, such as multivalency, high target retention and slower blood clearance, and as such, a variety of scFv formats have been generated. ScFvs can be administered by systemic injection for diagnostic and therapeutic purposes. They can be expressed in vivo through viral vectors in instances where large infection rates and sustenance of high levels of the antibody is required. ScFvs have found applications as tools for in vivo loss-of-function studies and inactivation of specific protein domains, diagnostic imaging, tumor therapy and treatment for neurodegenerative and infectious diseases. This review will focus on their in vivo applications. |
topic |
scFv recombinant antibodies in vivo expression adeno-associated virus (AAV) immunotherapy |
url |
http://www.mdpi.com/2073-4468/2/2/193 |
work_keys_str_mv |
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