A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathology
Background: Nucleic acids are potent stimulators of type I interferon (IFN-I) and antiviral defense, but may also promote pathological inflammation. A range of diseases are characterized by elevated IFN-I, including systemic lupus erythematosus (lupus). The DNA-activated cGAS-STING pathway is a majo...
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Elsevier
2021-04-01
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Series: | EBioMedicine |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396421001079 |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thaneas Prabakaran Anne Troldborg Sarinya Kumpunya Isara Alee Emilija Marinković Samuel J. Windross Ramya Nandakumar Ryo Narita Bao-cun Zhang Mikkel Carstensen Pichpisith Vejvisithsakul Mikkel H.S. Marqvorsen Marie B. Iversen Christian K. Holm Lars J. Østergaard Finn Skou Pedersen Trairak Pisitkun Rayk Behrendt Prapaporn Pisitkun Søren R. Paludan |
spellingShingle |
Thaneas Prabakaran Anne Troldborg Sarinya Kumpunya Isara Alee Emilija Marinković Samuel J. Windross Ramya Nandakumar Ryo Narita Bao-cun Zhang Mikkel Carstensen Pichpisith Vejvisithsakul Mikkel H.S. Marqvorsen Marie B. Iversen Christian K. Holm Lars J. Østergaard Finn Skou Pedersen Trairak Pisitkun Rayk Behrendt Prapaporn Pisitkun Søren R. Paludan A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathology EBioMedicine Lupus STING Innate immunity Type I interferon Inflammation Immunomodulatory therapy |
author_facet |
Thaneas Prabakaran Anne Troldborg Sarinya Kumpunya Isara Alee Emilija Marinković Samuel J. Windross Ramya Nandakumar Ryo Narita Bao-cun Zhang Mikkel Carstensen Pichpisith Vejvisithsakul Mikkel H.S. Marqvorsen Marie B. Iversen Christian K. Holm Lars J. Østergaard Finn Skou Pedersen Trairak Pisitkun Rayk Behrendt Prapaporn Pisitkun Søren R. Paludan |
author_sort |
Thaneas Prabakaran |
title |
A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathology |
title_short |
A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathology |
title_full |
A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathology |
title_fullStr |
A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathology |
title_full_unstemmed |
A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathology |
title_sort |
sting antagonist modulating the interaction with stim1 blocks er-to-golgi trafficking and inhibits lupus pathology |
publisher |
Elsevier |
series |
EBioMedicine |
issn |
2352-3964 |
publishDate |
2021-04-01 |
description |
Background: Nucleic acids are potent stimulators of type I interferon (IFN-I) and antiviral defense, but may also promote pathological inflammation. A range of diseases are characterized by elevated IFN-I, including systemic lupus erythematosus (lupus). The DNA-activated cGAS-STING pathway is a major IFN-I-inducing pathway, and activation of signaling is dependent on trafficking of STING from the ER to the Golgi. Methods: Here we used cell culture systems, a mouse lupus model, and material from lupus patients, to explore the mode of action of a STING antagonistic peptide, and its ability to modulate disease processes. Findings: We report that the peptide ISD017 selectively inhibits all known down-stream activities of STING, including IFN-I, inflammatory cytokines, autophagy, and apoptosis. ISD017 blocks the essential trafficking of STING from the ER to Golgi through a mechanism dependent on the STING ER retention factor STIM1. Importantly, ISD017 blocks STING activity in vivo and ameliorates disease development in a mouse model for lupus. Finally, ISD017 treatment blocks pathological cytokine responses in cells from lupus patients with elevated IFN-I levels. Interpretation: These data hold promise for beneficial use of STING-targeting therapy in lupus. Funding: The Novo Nordisk Foundation, The European Research Council, The Lundbeck Foundation, European Union under the Horizon 2020 Research, Deutsche Forschungsgemeinschaft, Chulalongkorn University. |
topic |
Lupus STING Innate immunity Type I interferon Inflammation Immunomodulatory therapy |
url |
http://www.sciencedirect.com/science/article/pii/S2352396421001079 |
work_keys_str_mv |
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doaj-da5f1e87afd543a2bd603e12507fe5092021-04-30T07:22:48ZengElsevierEBioMedicine2352-39642021-04-0166103314A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathologyThaneas Prabakaran0Anne Troldborg1Sarinya Kumpunya2Isara Alee3Emilija Marinković4Samuel J. Windross5Ramya Nandakumar6Ryo Narita7Bao-cun Zhang8Mikkel Carstensen9Pichpisith Vejvisithsakul10Mikkel H.S. Marqvorsen11Marie B. Iversen12Christian K. Holm13Lars J. Østergaard14Finn Skou Pedersen15Trairak Pisitkun16Rayk Behrendt17Prapaporn Pisitkun18Søren R. Paludan19Department of Biomedicine, Aarhus University, Aarhus DK-8000, DenmarkDepartment of Biomedicine, Aarhus University, Aarhus DK-8000, Denmark; Department of Clinical Medicine, Aarhus University, Aarhus DK-8000, Denmark; Department of Rheumatology, Aarhus University Hospital, Aarhus N 8200, DenmarkCenter of Excellence in Systems Biology, Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Inter-Department Program of Biomedical Sciences, Faculty of Graduate, Chulalongkorn University, Bangkok, ThailandCenter of Excellence in Systems Biology, Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Inter-Department Program of Biomedical Sciences, Faculty of Graduate, Chulalongkorn University, Bangkok, ThailandInstitute for Immunology, Faculty of Medicine, Technical University Dresden, Dresden, GermanyDepartment of Biomedicine, Aarhus University, Aarhus DK-8000, DenmarkDepartment of Biomedicine, Aarhus University, Aarhus DK-8000, DenmarkDepartment of Biomedicine, Aarhus University, Aarhus DK-8000, DenmarkDepartment of Biomedicine, Aarhus University, Aarhus DK-8000, DenmarkDepartment of Biomedicine, Aarhus University, Aarhus DK-8000, DenmarkDepartment of Biomedicine, Aarhus University, Aarhus DK-8000, Denmark; Section for Translational Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, ThailandDepartment of Biomedicine, Aarhus University, Aarhus DK-8000, DenmarkDepartment of Biomedicine, Aarhus University, Aarhus DK-8000, DenmarkDepartment of Biomedicine, Aarhus University, Aarhus DK-8000, DenmarkDepartment of Clinical Medicine, Aarhus University, Aarhus DK-8000, Denmark; Department of Infectious Diseases, Aarhus University Hospital, Aarhus N 8200, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Aarhus DK-8000, DenmarkCenter of Excellence in Systems Biology, Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, ThailandInstitute for Immunology, Faculty of Medicine, Technical University Dresden, Dresden, GermanySection for Translational Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand; Division of Allergy, Immunology, and Rheumatology, Department of Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, ThailandDepartment of Biomedicine, Aarhus University, Aarhus DK-8000, Denmark; Corresponding author.Background: Nucleic acids are potent stimulators of type I interferon (IFN-I) and antiviral defense, but may also promote pathological inflammation. A range of diseases are characterized by elevated IFN-I, including systemic lupus erythematosus (lupus). The DNA-activated cGAS-STING pathway is a major IFN-I-inducing pathway, and activation of signaling is dependent on trafficking of STING from the ER to the Golgi. Methods: Here we used cell culture systems, a mouse lupus model, and material from lupus patients, to explore the mode of action of a STING antagonistic peptide, and its ability to modulate disease processes. Findings: We report that the peptide ISD017 selectively inhibits all known down-stream activities of STING, including IFN-I, inflammatory cytokines, autophagy, and apoptosis. ISD017 blocks the essential trafficking of STING from the ER to Golgi through a mechanism dependent on the STING ER retention factor STIM1. Importantly, ISD017 blocks STING activity in vivo and ameliorates disease development in a mouse model for lupus. Finally, ISD017 treatment blocks pathological cytokine responses in cells from lupus patients with elevated IFN-I levels. Interpretation: These data hold promise for beneficial use of STING-targeting therapy in lupus. Funding: The Novo Nordisk Foundation, The European Research Council, The Lundbeck Foundation, European Union under the Horizon 2020 Research, Deutsche Forschungsgemeinschaft, Chulalongkorn University.http://www.sciencedirect.com/science/article/pii/S2352396421001079LupusSTINGInnate immunityType I interferonInflammationImmunomodulatory therapy |