Quantitative assessment of disease markers using the naked eye: point-of-care testing with gas generation-based biosensor immunochromatographic strips
Abstract Background Immunochromatographic strips (ICSs) are a practical tool commonly used in point-of-care testing (POCT) applications. However, ICSs that are currently available have low sensitivity and require expensive equipment for quantitative analysis. These limitations prohibit their extensi...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | http://link.springer.com/article/10.1186/s12951-019-0493-z |
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doaj-da8784a45cff43008f4ea32a6dbbaef4 |
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Article |
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DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Qiangqiang Fu Ze Wu Jingxia Li Zengfeng Wu Hui Zhong Quanli Yang Qihui Liu Zonghua Liu Lianghe Sheng Meng Xu Tingting Li Zhinan Yin Yangzhe Wu |
| spellingShingle |
Qiangqiang Fu Ze Wu Jingxia Li Zengfeng Wu Hui Zhong Quanli Yang Qihui Liu Zonghua Liu Lianghe Sheng Meng Xu Tingting Li Zhinan Yin Yangzhe Wu Quantitative assessment of disease markers using the naked eye: point-of-care testing with gas generation-based biosensor immunochromatographic strips Journal of Nanobiotechnology Immunochromatographic strips Gas generation-based biosensors C-reactive protein Au@Pt core/shell nanoparticles |
| author_facet |
Qiangqiang Fu Ze Wu Jingxia Li Zengfeng Wu Hui Zhong Quanli Yang Qihui Liu Zonghua Liu Lianghe Sheng Meng Xu Tingting Li Zhinan Yin Yangzhe Wu |
| author_sort |
Qiangqiang Fu |
| title |
Quantitative assessment of disease markers using the naked eye: point-of-care testing with gas generation-based biosensor immunochromatographic strips |
| title_short |
Quantitative assessment of disease markers using the naked eye: point-of-care testing with gas generation-based biosensor immunochromatographic strips |
| title_full |
Quantitative assessment of disease markers using the naked eye: point-of-care testing with gas generation-based biosensor immunochromatographic strips |
| title_fullStr |
Quantitative assessment of disease markers using the naked eye: point-of-care testing with gas generation-based biosensor immunochromatographic strips |
| title_full_unstemmed |
Quantitative assessment of disease markers using the naked eye: point-of-care testing with gas generation-based biosensor immunochromatographic strips |
| title_sort |
quantitative assessment of disease markers using the naked eye: point-of-care testing with gas generation-based biosensor immunochromatographic strips |
| publisher |
BMC |
| series |
Journal of Nanobiotechnology |
| issn |
1477-3155 |
| publishDate |
2019-05-01 |
| description |
Abstract Background Immunochromatographic strips (ICSs) are a practical tool commonly used in point-of-care testing (POCT) applications. However, ICSs that are currently available have low sensitivity and require expensive equipment for quantitative analysis. These limitations prohibit their extensive use in areas where medical resources are scarce. Methods We developed a novel POCT platform by integrating a gas generation biosensor with Au@Pt Core/Shell nanoparticle (Au@PtNPs)-based ICSs (G-ICSs). The resulting G-ICSs enabled the convenient and quantitative assessment of a target protein using the naked eye, without the need for auxiliary equipment or complicated computation. To assess this platform, C-reactive protein (CRP), a biomarker commonly used for the diagnosis of acute, infectious diseases was chosen as a proof-of-concept test. Results The linear detection range (LDR) of the G-ICSs for CRP was 0.05–6.25 μg/L with a limit of detection (LOD) of 0.041 μg/L. The G-ICSs had higher sensitivity and wider LDR when compared with commonly used AuNPs and fluorescent-based ICSs. When compared with results from a chemiluminescent immunoassay, G-ICS concordance rates for CRP detection in serum samples ranged from 93.72 to 110.99%. Conclusions These results demonstrated that G-ICSs have wide applicability in family diagnosis and community medical institutions, especially in areas with poor medical resources. |
| topic |
Immunochromatographic strips Gas generation-based biosensors C-reactive protein Au@Pt core/shell nanoparticles |
| url |
http://link.springer.com/article/10.1186/s12951-019-0493-z |
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doaj-da8784a45cff43008f4ea32a6dbbaef42020-11-25T03:23:49ZengBMCJournal of Nanobiotechnology1477-31552019-05-0117111010.1186/s12951-019-0493-zQuantitative assessment of disease markers using the naked eye: point-of-care testing with gas generation-based biosensor immunochromatographic stripsQiangqiang Fu0Ze Wu1Jingxia Li2Zengfeng Wu3Hui Zhong4Quanli Yang5Qihui Liu6Zonghua Liu7Lianghe Sheng8Meng Xu9Tingting Li10Zhinan Yin11Yangzhe Wu12The First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy and, and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan UniversityDepartment of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical UniversityThe First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy and, and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan UniversityThe First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy and, and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan UniversityThe First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy and, and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan UniversityThe First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy and, and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan UniversityThe First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy and, and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan UniversityThe First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy and, and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan UniversityThe First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy and, and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan UniversityThe First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy and, and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan UniversityDepartment of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical UniversityThe First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy and, and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan UniversityThe First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy and, and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan UniversityAbstract Background Immunochromatographic strips (ICSs) are a practical tool commonly used in point-of-care testing (POCT) applications. However, ICSs that are currently available have low sensitivity and require expensive equipment for quantitative analysis. These limitations prohibit their extensive use in areas where medical resources are scarce. Methods We developed a novel POCT platform by integrating a gas generation biosensor with Au@Pt Core/Shell nanoparticle (Au@PtNPs)-based ICSs (G-ICSs). The resulting G-ICSs enabled the convenient and quantitative assessment of a target protein using the naked eye, without the need for auxiliary equipment or complicated computation. To assess this platform, C-reactive protein (CRP), a biomarker commonly used for the diagnosis of acute, infectious diseases was chosen as a proof-of-concept test. Results The linear detection range (LDR) of the G-ICSs for CRP was 0.05–6.25 μg/L with a limit of detection (LOD) of 0.041 μg/L. The G-ICSs had higher sensitivity and wider LDR when compared with commonly used AuNPs and fluorescent-based ICSs. When compared with results from a chemiluminescent immunoassay, G-ICS concordance rates for CRP detection in serum samples ranged from 93.72 to 110.99%. Conclusions These results demonstrated that G-ICSs have wide applicability in family diagnosis and community medical institutions, especially in areas with poor medical resources.http://link.springer.com/article/10.1186/s12951-019-0493-zImmunochromatographic stripsGas generation-based biosensorsC-reactive proteinAu@Pt core/shell nanoparticles |