The Antioxidant Capacity In Vitro and In Vivo of Polysaccharides from <i>Bergenia emeiensis</i>
Polysaccharides from <i>Bergenia emeiensis</i> (PBE) showed a robust antioxidant ability on scavenging free radicals in vitro. However, the further antioxidant potential in cell level and in vivo was still unknown. Therefore, in this present study, the protective effect of PBE on human c...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-10-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/20/7456 |
| Summary: | Polysaccharides from <i>Bergenia emeiensis</i> (PBE) showed a robust antioxidant ability on scavenging free radicals in vitro. However, the further antioxidant potential in cell level and in vivo was still unknown. Therefore, in this present study, the protective effect of PBE on human cervical carcinoma cell (Hela) cells and <i>Caenorhabditis elegans</i> against oxidative stress was evaluated. The results showed PBE could reduce the reactive oxygen species (ROS) level in Hela cells and promote the mitochondrial membrane potential. Then, the cell apoptosis was reduced. Moreover, PBE could enhance the survival of <i>C. elegans</i> under thermal stress to 13.44%, and significantly reduce the ROS level, which was connected with the overexpression of <i>sod-3</i> and the increased nuclear localization of <i>daf-16</i> transcription factor. Therefore, PBE exhibited a strong antioxidant capacity in the cellular level and for a whole organism. Thus, polysaccharides from <i>B. emeiensis</i> have natural potential to be a safe antioxidant. |
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| ISSN: | 1661-6596 1422-0067 |