Tissue factor-dependent coagulation activation by heme: A thromboelastometry study.
Heme has been characterized as potent trigger of inflammation. In hemostasis, although heme has been shown to both induce and inhibit different compartments of hemostasis, its net effect on the hemostatic balance, and the biological relevance of these effects remain to be determined. Herein we evalu...
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doaj-da969806bb234cc88236e0e3cbba4afd2020-11-25T01:31:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01124e017650510.1371/journal.pone.0176505Tissue factor-dependent coagulation activation by heme: A thromboelastometry study.Gleice Regina de SouzaBidossessi Wilfried HounkpeMaiara Marx Luz FiusaMarina Pereira ColellaJoyce M Annichino-BizzacchiFabiola TrainaFernando Ferreira CostaErich Vinicius De PaulaHeme has been characterized as potent trigger of inflammation. In hemostasis, although heme has been shown to both induce and inhibit different compartments of hemostasis, its net effect on the hemostatic balance, and the biological relevance of these effects remain to be determined. Herein we evaluated the effect of heme on hemostasis using a global assay able to generate clinically relevant data in several other complex hemostatic diseases. Citrated whole blood samples from healthy participants were stimulated by heme or vehicle and incubated for 4h at 37°C. Rotational thromboelastometry was immediately performed. The participation of tissue factor in coagulation activation was evaluated using inhibitory antibody. Heme was able of inducing ex vivo coagulation activation in whole blood, affecting predominantly parameters associated with the initial phases of clot formation. This activation effect was at least partially dependent on hematopoietic tissue factor, since the effects of heme were partially abrogated by the inhibition of human tissue factor. In conclusion, using a global hemostasis assay, our study confirmed that heme is able to activate coagulation in whole blood, in a tissue factor-dependent way. These findings could explain the disturbance in hemostatic balance observed in conditions associated with the release of heme such as sickle cell disease.http://europepmc.org/articles/PMC5402930?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gleice Regina de Souza Bidossessi Wilfried Hounkpe Maiara Marx Luz Fiusa Marina Pereira Colella Joyce M Annichino-Bizzacchi Fabiola Traina Fernando Ferreira Costa Erich Vinicius De Paula |
spellingShingle |
Gleice Regina de Souza Bidossessi Wilfried Hounkpe Maiara Marx Luz Fiusa Marina Pereira Colella Joyce M Annichino-Bizzacchi Fabiola Traina Fernando Ferreira Costa Erich Vinicius De Paula Tissue factor-dependent coagulation activation by heme: A thromboelastometry study. PLoS ONE |
author_facet |
Gleice Regina de Souza Bidossessi Wilfried Hounkpe Maiara Marx Luz Fiusa Marina Pereira Colella Joyce M Annichino-Bizzacchi Fabiola Traina Fernando Ferreira Costa Erich Vinicius De Paula |
author_sort |
Gleice Regina de Souza |
title |
Tissue factor-dependent coagulation activation by heme: A thromboelastometry study. |
title_short |
Tissue factor-dependent coagulation activation by heme: A thromboelastometry study. |
title_full |
Tissue factor-dependent coagulation activation by heme: A thromboelastometry study. |
title_fullStr |
Tissue factor-dependent coagulation activation by heme: A thromboelastometry study. |
title_full_unstemmed |
Tissue factor-dependent coagulation activation by heme: A thromboelastometry study. |
title_sort |
tissue factor-dependent coagulation activation by heme: a thromboelastometry study. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
Heme has been characterized as potent trigger of inflammation. In hemostasis, although heme has been shown to both induce and inhibit different compartments of hemostasis, its net effect on the hemostatic balance, and the biological relevance of these effects remain to be determined. Herein we evaluated the effect of heme on hemostasis using a global assay able to generate clinically relevant data in several other complex hemostatic diseases. Citrated whole blood samples from healthy participants were stimulated by heme or vehicle and incubated for 4h at 37°C. Rotational thromboelastometry was immediately performed. The participation of tissue factor in coagulation activation was evaluated using inhibitory antibody. Heme was able of inducing ex vivo coagulation activation in whole blood, affecting predominantly parameters associated with the initial phases of clot formation. This activation effect was at least partially dependent on hematopoietic tissue factor, since the effects of heme were partially abrogated by the inhibition of human tissue factor. In conclusion, using a global hemostasis assay, our study confirmed that heme is able to activate coagulation in whole blood, in a tissue factor-dependent way. These findings could explain the disturbance in hemostatic balance observed in conditions associated with the release of heme such as sickle cell disease. |
url |
http://europepmc.org/articles/PMC5402930?pdf=render |
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