Identification of RD5-Encoded Mycobacterium tuberculosis Proteins As B-Cell Antigens Used for Serodiagnosis of Tuberculosis
Comparative genomic studies have identified several Mycobacterium tuberculosis-specific genomic regions of difference (RDs) which are absent in the vaccine strains of Mycobacterium bovis BCG and which may be useful in the specific diagnosis of tuberculosis (TB). In this study, all encoded proteins f...
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Online Access: | http://dx.doi.org/10.1155/2012/738043 |
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doaj-da9d5cd328914b9db3d147a0928141f22020-11-24T22:48:17ZengHindawi LimitedClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/738043738043Identification of RD5-Encoded Mycobacterium tuberculosis Proteins As B-Cell Antigens Used for Serodiagnosis of TuberculosisMiao-Miao Zhang0Jun-Wei Zhao1Zhan-Qiang Sun2Jun Liu3Xiao-Kui Guo4Wen-Di Liu5Shu-Lin Zhang6Department of Microbiology and Parasitology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaDepartment of Microbiology and Parasitology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaShanghai Kaidi Biotech Co., Ltd., Shanghai 200433, ChinaWuxi No. 5 People’s Hospital, Wuxi 214005, ChinaDepartment of Microbiology and Parasitology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaDepartment of Immunology, Xinxiang Medical University, Xinxiang 453000, ChinaDepartment of Microbiology and Parasitology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaComparative genomic studies have identified several Mycobacterium tuberculosis-specific genomic regions of difference (RDs) which are absent in the vaccine strains of Mycobacterium bovis BCG and which may be useful in the specific diagnosis of tuberculosis (TB). In this study, all encoded proteins from DNA segment RD5 of Mycobacterium tuberculosis, that is, Rv3117–Rv3121, were recombined and evaluated by enzyme-linked immunosorbent assays for antibody reactivity with sera from HIV-negative pulmonary TB patients (n=60) and healthy controls (n=32). The results identified two immunodominant antigens, that is, Rv3117 and Rv3120, both of which revealed a statistically significant antigenic distinction between healthy controls and TB patients (P<0.05). In comparison with the well-known early-secreted antigen target 6 kDa (ESAT-6) (sensitivity 21.7%, specificity 90.6%), the higher detection sensitivity and higher specificity were achieved (Rv3117: sensitivity 25%, specificity 96.9%; Rv3120: sensitivity 31.7%, specificity 96.9%). Thus, the results highlight the immunosensitive and immunospecific nature of Rv3117 and Rv3120 and indicate promise for their use in the serodiagnosis of TB.http://dx.doi.org/10.1155/2012/738043 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Miao-Miao Zhang Jun-Wei Zhao Zhan-Qiang Sun Jun Liu Xiao-Kui Guo Wen-Di Liu Shu-Lin Zhang |
spellingShingle |
Miao-Miao Zhang Jun-Wei Zhao Zhan-Qiang Sun Jun Liu Xiao-Kui Guo Wen-Di Liu Shu-Lin Zhang Identification of RD5-Encoded Mycobacterium tuberculosis Proteins As B-Cell Antigens Used for Serodiagnosis of Tuberculosis Clinical and Developmental Immunology |
author_facet |
Miao-Miao Zhang Jun-Wei Zhao Zhan-Qiang Sun Jun Liu Xiao-Kui Guo Wen-Di Liu Shu-Lin Zhang |
author_sort |
Miao-Miao Zhang |
title |
Identification of RD5-Encoded Mycobacterium tuberculosis Proteins As B-Cell Antigens Used for Serodiagnosis of Tuberculosis |
title_short |
Identification of RD5-Encoded Mycobacterium tuberculosis Proteins As B-Cell Antigens Used for Serodiagnosis of Tuberculosis |
title_full |
Identification of RD5-Encoded Mycobacterium tuberculosis Proteins As B-Cell Antigens Used for Serodiagnosis of Tuberculosis |
title_fullStr |
Identification of RD5-Encoded Mycobacterium tuberculosis Proteins As B-Cell Antigens Used for Serodiagnosis of Tuberculosis |
title_full_unstemmed |
Identification of RD5-Encoded Mycobacterium tuberculosis Proteins As B-Cell Antigens Used for Serodiagnosis of Tuberculosis |
title_sort |
identification of rd5-encoded mycobacterium tuberculosis proteins as b-cell antigens used for serodiagnosis of tuberculosis |
publisher |
Hindawi Limited |
series |
Clinical and Developmental Immunology |
issn |
1740-2522 1740-2530 |
publishDate |
2012-01-01 |
description |
Comparative genomic studies have identified several Mycobacterium tuberculosis-specific genomic regions of difference (RDs) which are absent in the vaccine strains of Mycobacterium bovis BCG and which may be useful in the specific diagnosis of tuberculosis (TB). In this study, all encoded proteins from DNA segment RD5 of Mycobacterium tuberculosis, that is, Rv3117–Rv3121, were recombined and evaluated by enzyme-linked immunosorbent assays for antibody reactivity with sera from HIV-negative pulmonary TB patients (n=60) and healthy controls (n=32). The results identified two immunodominant antigens, that is, Rv3117 and Rv3120, both of which revealed a statistically significant antigenic distinction between healthy controls and TB patients (P<0.05). In comparison with the well-known early-secreted antigen target 6 kDa (ESAT-6) (sensitivity 21.7%, specificity 90.6%), the higher detection sensitivity and higher specificity were achieved (Rv3117: sensitivity 25%, specificity 96.9%; Rv3120: sensitivity 31.7%, specificity 96.9%). Thus, the results highlight the immunosensitive and immunospecific nature of Rv3117 and Rv3120 and indicate promise for their use in the serodiagnosis of TB. |
url |
http://dx.doi.org/10.1155/2012/738043 |
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