Simultaneous quantification of stevioside and rebaudioside A in different stevia samples collected from the Indian subcontinent
Background: A high performance thin layer chromatographic (HPTLC) method was developed for simultaneous estimation of stevioside and rebaudioside A in Stevia rebaudiana samples collected from different regions of Indian subcontinent. Materials and Methods: The separation was achieved by using aceton...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2012-01-01
|
Series: | Journal of Pharmacy and Bioallied Sciences |
Subjects: | |
Online Access: | http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2012;volume=4;issue=4;spage=276;epage=281;aulast=Chester |
id |
doaj-dab563b8d61842a8899f371674afb3da |
---|---|
record_format |
Article |
spelling |
doaj-dab563b8d61842a8899f371674afb3da2020-11-24T21:30:40ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0975-74060976-48792012-01-014427628110.4103/0975-7406.103236Simultaneous quantification of stevioside and rebaudioside A in different stevia samples collected from the Indian subcontinentKarishma ChesterEnnus T TamboliMhaveer SinghSayeed AhmadBackground: A high performance thin layer chromatographic (HPTLC) method was developed for simultaneous estimation of stevioside and rebaudioside A in Stevia rebaudiana samples collected from different regions of Indian subcontinent. Materials and Methods: The separation was achieved by using acetone: ethyl acetate: water (5:4:1, v/v/v) as the solvent system on precoated silica gel 60 F 254 TLC plates. The densitometric quantification of stevia glycosides was carried out at wavelength 360 nm in absorption mode after spraying with anisaldehyde sulphuric acid as detecting reagent. Results: The well resolved peaks for stevioside and rebaudioside A were observed at R f values 0.31± 0.02 and 0.21± 0.02 respectively. The calibration curves were found linear with a wide range of concentration 100 - 2000 ng spot -1 with good correlation coefficient 0.996 and 0.991 for stevioside and rebaudioside A, respectively. Conclusions: The proposed method was validated as per the ICH (International Conferences on Harmonization) guidelines and found simple, sensitive, economic, reproducible, robust and accurate for quantitative analysis of stevia glycosides, which can be applied for quality control of stevia as well as to check.http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2012;volume=4;issue=4;spage=276;epage=281;aulast=ChesterDensitometryHPTLCrebaudioside AStevia rebaudianastevioside |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Karishma Chester Ennus T Tamboli Mhaveer Singh Sayeed Ahmad |
spellingShingle |
Karishma Chester Ennus T Tamboli Mhaveer Singh Sayeed Ahmad Simultaneous quantification of stevioside and rebaudioside A in different stevia samples collected from the Indian subcontinent Journal of Pharmacy and Bioallied Sciences Densitometry HPTLC rebaudioside A Stevia rebaudiana stevioside |
author_facet |
Karishma Chester Ennus T Tamboli Mhaveer Singh Sayeed Ahmad |
author_sort |
Karishma Chester |
title |
Simultaneous quantification of stevioside and rebaudioside A in different stevia samples collected from the Indian subcontinent |
title_short |
Simultaneous quantification of stevioside and rebaudioside A in different stevia samples collected from the Indian subcontinent |
title_full |
Simultaneous quantification of stevioside and rebaudioside A in different stevia samples collected from the Indian subcontinent |
title_fullStr |
Simultaneous quantification of stevioside and rebaudioside A in different stevia samples collected from the Indian subcontinent |
title_full_unstemmed |
Simultaneous quantification of stevioside and rebaudioside A in different stevia samples collected from the Indian subcontinent |
title_sort |
simultaneous quantification of stevioside and rebaudioside a in different stevia samples collected from the indian subcontinent |
publisher |
Wolters Kluwer Medknow Publications |
series |
Journal of Pharmacy and Bioallied Sciences |
issn |
0975-7406 0976-4879 |
publishDate |
2012-01-01 |
description |
Background: A high performance thin layer chromatographic (HPTLC) method was developed for simultaneous estimation of stevioside and rebaudioside A in Stevia rebaudiana samples collected from different regions of Indian subcontinent. Materials and Methods: The separation was achieved by using acetone: ethyl acetate: water (5:4:1, v/v/v) as the solvent system on precoated silica gel 60 F 254 TLC plates. The densitometric quantification of stevia glycosides was carried out at wavelength 360 nm in absorption mode after spraying with anisaldehyde sulphuric acid as detecting reagent. Results: The well resolved peaks for stevioside and rebaudioside A were observed at R f values 0.31± 0.02 and 0.21± 0.02 respectively. The calibration curves were found linear with a wide range of concentration 100 - 2000 ng spot -1 with good correlation coefficient 0.996 and 0.991 for stevioside and rebaudioside A, respectively. Conclusions: The proposed method was validated as per the ICH (International Conferences on Harmonization) guidelines and found simple, sensitive, economic, reproducible, robust and accurate for quantitative analysis of stevia glycosides, which can be applied for quality control of stevia as well as to check. |
topic |
Densitometry HPTLC rebaudioside A Stevia rebaudiana stevioside |
url |
http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2012;volume=4;issue=4;spage=276;epage=281;aulast=Chester |
work_keys_str_mv |
AT karishmachester simultaneousquantificationofsteviosideandrebaudiosideaindifferentsteviasamplescollectedfromtheindiansubcontinent AT ennusttamboli simultaneousquantificationofsteviosideandrebaudiosideaindifferentsteviasamplescollectedfromtheindiansubcontinent AT mhaveersingh simultaneousquantificationofsteviosideandrebaudiosideaindifferentsteviasamplescollectedfromtheindiansubcontinent AT sayeedahmad simultaneousquantificationofsteviosideandrebaudiosideaindifferentsteviasamplescollectedfromtheindiansubcontinent |
_version_ |
1725962293092548608 |