Evaluating the Biofilm Ability by Microtiter Plate Method in Uropathogenic Escherichia Coli Isolated from Patients with Urinary Tract Infection with Urinary Stones

• Main Authors: M Bakhshi, H Jamali
• Format: Article
• Language: fas
• Published: Yasuj University Of Medical Sciences 2020-08-01
• Series: Armaghane Danesh Bimonthly Journal
• Subjects: urinary tract infections; uropathogenic e. coli; biofilm
• Online Access: http://armaghanj.yums.ac.ir/article-1-2493-en.html

Background and aim: Urinary tract infections are one of the most commonly reported nosocomial infections caused by colonization of E. coli in the mucosal epithelium and in the formation of microbial biofilms, which damage the host tissue. The aim of this study was to determine the amount of biofilm formation of uropathogenic E. coli based on urinary tract stones of patients with urinary tract infection.   Methods: This research is a quasi-experimental study using pre-test-post-test design which was conducted in 2015, 380 patients with positive urine culture with Escherichia coli bacteria were studied. Among the total population, 34 patients had urinary stone and urinary tract infection with E. coli simultaneously. These rocks were sent to the laboratory in accordance with sterile conditions. For long-term storage of bacteria for collection and other tests so that the bacteria themselves are not damaged, they were kept in glycerol stock. To determine the biofilm formation capacity, this bacterium was investigated by Microtiter Plate method.   Results:The results indicated that a total of 34 stone samples were examined using conventional methods containing uropathogenic E. coli. These microorganisms showed a low biofilm strength of 18%, very low power, 20% moderate power and 59% high power.   Conclusion: In present study, it was revealed that the concomitant presence of infection with urinary stones as a suitable surface and substrate for Escherichia coli, and as a result of the formation of biofilm of this bacterium on the surface of the stone, triggered further urinary tract infection and antibiotic resistance in some strains initiating urinary tract infections.