Assessment of innate immunity by phagocytic activity in non-descript and Sahiwal cattle in Chhattisgarh
Aim: The objective of the research was to assess the innate immunity status of non-descript (ND) cattle in comparison to Sahiwal cattle in Chattisgarh by the determination of the phagocytosis activity (PA) and opsonocytophagic index (OI). Materials and Methods: In-vitro phagocytic activity of neutr...
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doaj-daca40bf96b54a1c92ccacd9317544b82021-08-02T12:54:01ZengVeterinary WorldVeterinary World0972-89882231-09162014-11-0171194394710.14202/vetworld.2014.943-947Assessment of innate immunity by phagocytic activity in non-descript and Sahiwal cattle in ChhattisgarhGovina Dewangan0S. D. Hirpurkar1Devesh Kumar Giri 2Deepak Kumar Kashyap3Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Durg, Chhattisgarh, India; govinadewangan@gmail.comDepartment of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Durg, Chhattisgarh, India; smpuhir@yahoo.comDepartment of Veterinary Pathology, Uttar Pradesh, Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evan Go Anusandhan Sansthan, Mathura, Uttar Pradesh, India; giri.devesh18@gmail.comDepartment of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Arawali Veterinary College, Sikar, Rajasthan, India; deepakkashyap31@gmail.comAim: The objective of the research was to assess the innate immunity status of non-descript (ND) cattle in comparison to Sahiwal cattle in Chattisgarh by the determination of the phagocytosis activity (PA) and opsonocytophagic index (OI). Materials and Methods: In-vitro phagocytic activity of neutrophil was studied in ND and Sahiwal (S) cattle after dividing them in groups viz. ND-I, ND-II, S-I and S-II. Neutrophils were separated from the blood and activated with Staphylococcus aureus. PA and OI were observed under high power of a microscope at ×1000 magnification. Results: PA (percent) in groups ND-I, ND-II, S-I and S-II was 73.00±1.03, 82.25±1.51, 80.75±1.36 and 82.37±1.27, respectively which vary significantly (p<0.01), with respect to age. The observations regarding the OI (bacteria per neutrophil) in groups ND-I, ND-II, S-I and S-II was 7.17±0.04, 7.76±0.09, 7.02±0.04 and 7.79±0.06, respectively. Age difference was evident, i.e., OI increases as age increases. Conclusion: Innate immunity plays a vital role in host defense against diseases. Neutrophil kills the invading pathogen by phagocytic action. PA and OI is an important tool for the assessing innate immune status. After coincubation of neutrophil with bacterial cells, PA was significantly lower (p<0.01) in ND-I, calves group then other groups. Further, the result of OI clearly indicates that OI has increased as age increases in both breeds. The study will, therefore, generate first hand information on ND cattle along with their relative statistical differences, if any, when compared with Sahiwal.http://www.veterinaryworld.org/Vol.7/November-2014/8.pdfinnate immunitynon-descript cattleopsonocytophagic indexphagocytosis activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Govina Dewangan S. D. Hirpurkar Devesh Kumar Giri Deepak Kumar Kashyap |
spellingShingle |
Govina Dewangan S. D. Hirpurkar Devesh Kumar Giri Deepak Kumar Kashyap Assessment of innate immunity by phagocytic activity in non-descript and Sahiwal cattle in Chhattisgarh Veterinary World innate immunity non-descript cattle opsonocytophagic index phagocytosis activity |
author_facet |
Govina Dewangan S. D. Hirpurkar Devesh Kumar Giri Deepak Kumar Kashyap |
author_sort |
Govina Dewangan |
title |
Assessment of innate immunity by phagocytic activity in non-descript and Sahiwal cattle in Chhattisgarh |
title_short |
Assessment of innate immunity by phagocytic activity in non-descript and Sahiwal cattle in Chhattisgarh |
title_full |
Assessment of innate immunity by phagocytic activity in non-descript and Sahiwal cattle in Chhattisgarh |
title_fullStr |
Assessment of innate immunity by phagocytic activity in non-descript and Sahiwal cattle in Chhattisgarh |
title_full_unstemmed |
Assessment of innate immunity by phagocytic activity in non-descript and Sahiwal cattle in Chhattisgarh |
title_sort |
assessment of innate immunity by phagocytic activity in non-descript and sahiwal cattle in chhattisgarh |
publisher |
Veterinary World |
series |
Veterinary World |
issn |
0972-8988 2231-0916 |
publishDate |
2014-11-01 |
description |
Aim: The objective of the research was to assess the innate immunity status of non-descript (ND) cattle in comparison to Sahiwal cattle in Chattisgarh by the determination of the phagocytosis activity (PA) and opsonocytophagic index (OI).
Materials and Methods: In-vitro phagocytic activity of neutrophil was studied in ND and Sahiwal (S) cattle after dividing them in groups viz. ND-I, ND-II, S-I and S-II. Neutrophils were separated from the blood and activated with Staphylococcus aureus. PA and OI were observed under high power of a microscope at ×1000 magnification.
Results: PA (percent) in groups ND-I, ND-II, S-I and S-II was 73.00±1.03, 82.25±1.51, 80.75±1.36 and 82.37±1.27, respectively which vary significantly (p<0.01), with respect to age. The observations regarding the OI (bacteria per neutrophil) in groups ND-I, ND-II, S-I and S-II was 7.17±0.04, 7.76±0.09, 7.02±0.04 and 7.79±0.06, respectively. Age difference was evident, i.e., OI increases as age increases.
Conclusion: Innate immunity plays a vital role in host defense against diseases. Neutrophil kills the invading pathogen by phagocytic action. PA and OI is an important tool for the assessing innate immune status. After coincubation of neutrophil with bacterial cells, PA was significantly lower (p<0.01) in ND-I, calves group then other groups. Further, the result of OI clearly indicates that OI has increased as age increases in both breeds. The study will, therefore, generate first hand information on ND cattle along with their relative statistical differences, if any, when compared with Sahiwal. |
topic |
innate immunity non-descript cattle opsonocytophagic index phagocytosis activity |
url |
http://www.veterinaryworld.org/Vol.7/November-2014/8.pdf |
work_keys_str_mv |
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