Biosynthesis, Characterization of Some Combined Nanoparticles, and Its Biocide Potency against a Broad Spectrum of Pathogens
The development of environmentally benign procedures for the synthesis of metallic nanoparticles (NPs) is a vital aspect in bionanotechnology applications for health care and the environment. This study describes the biosynthesis of Ag, Co, Ni, and Zn NPs by employing nanobiofactory Proteus mirabili...
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doaj-dad25412ea934452b93a33af2fec7c952020-11-24T22:30:24ZengHindawi LimitedJournal of Nanomaterials1687-41101687-41292018-01-01201810.1155/2018/52638145263814Biosynthesis, Characterization of Some Combined Nanoparticles, and Its Biocide Potency against a Broad Spectrum of PathogensMarwa Eltarahony0Sahar Zaki1Marwa ElKady2Desouky Abd-El-Haleem3Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Borg El Arab, Alexandria, EgyptEnvironmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Borg El Arab, Alexandria, EgyptChemical and Petrochemical Engineering Department, Egypt-Japan University for Science and Technology, New Borg El-Arab City, Alexandria, EgyptEnvironmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Borg El Arab, Alexandria, EgyptThe development of environmentally benign procedures for the synthesis of metallic nanoparticles (NPs) is a vital aspect in bionanotechnology applications for health care and the environment. This study describes the biosynthesis of Ag, Co, Ni, and Zn NPs by employing nanobiofactory Proteus mirabilis strain 10B. The physicochemical characterization UV-visible spectroscopy, scanning electron microscopy-energy-dispersive X-ray microanalysis (EDX), X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS) technique including ζ potential, and polydispersity index (PDI) confirmed the formation of pure, stable monodisperse quasi-spherical oxide NPs of corresponding metals. The antimicrobial activity of biofabricated NPs was assessed against Gram-negative and Gram-positive bacteria, biofilm, yeast, mold, and algae via a well diffusion method. The results displayed significant antagonistic activity in comparison to their bulk and commercial antibiotics. Interestingly, the combined NPs exhibited promising synergistic biocide efficiency against examined pathogens which encourages their applications in adjuvant therapy and water/wastewater purification for controlling multiple drug-resistant microorganisms. To the best of our knowledge, no previous study reported the synthesis of semiconductor NPs by Proteus mirabilis and the biocide potency of combined NPs against a broad spectrum of pathogens not reported previously.http://dx.doi.org/10.1155/2018/5263814 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marwa Eltarahony Sahar Zaki Marwa ElKady Desouky Abd-El-Haleem |
spellingShingle |
Marwa Eltarahony Sahar Zaki Marwa ElKady Desouky Abd-El-Haleem Biosynthesis, Characterization of Some Combined Nanoparticles, and Its Biocide Potency against a Broad Spectrum of Pathogens Journal of Nanomaterials |
author_facet |
Marwa Eltarahony Sahar Zaki Marwa ElKady Desouky Abd-El-Haleem |
author_sort |
Marwa Eltarahony |
title |
Biosynthesis, Characterization of Some Combined Nanoparticles, and Its Biocide Potency against a Broad Spectrum of Pathogens |
title_short |
Biosynthesis, Characterization of Some Combined Nanoparticles, and Its Biocide Potency against a Broad Spectrum of Pathogens |
title_full |
Biosynthesis, Characterization of Some Combined Nanoparticles, and Its Biocide Potency against a Broad Spectrum of Pathogens |
title_fullStr |
Biosynthesis, Characterization of Some Combined Nanoparticles, and Its Biocide Potency against a Broad Spectrum of Pathogens |
title_full_unstemmed |
Biosynthesis, Characterization of Some Combined Nanoparticles, and Its Biocide Potency against a Broad Spectrum of Pathogens |
title_sort |
biosynthesis, characterization of some combined nanoparticles, and its biocide potency against a broad spectrum of pathogens |
publisher |
Hindawi Limited |
series |
Journal of Nanomaterials |
issn |
1687-4110 1687-4129 |
publishDate |
2018-01-01 |
description |
The development of environmentally benign procedures for the synthesis of metallic nanoparticles (NPs) is a vital aspect in bionanotechnology applications for health care and the environment. This study describes the biosynthesis of Ag, Co, Ni, and Zn NPs by employing nanobiofactory Proteus mirabilis strain 10B. The physicochemical characterization UV-visible spectroscopy, scanning electron microscopy-energy-dispersive X-ray microanalysis (EDX), X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS) technique including ζ potential, and polydispersity index (PDI) confirmed the formation of pure, stable monodisperse quasi-spherical oxide NPs of corresponding metals. The antimicrobial activity of biofabricated NPs was assessed against Gram-negative and Gram-positive bacteria, biofilm, yeast, mold, and algae via a well diffusion method. The results displayed significant antagonistic activity in comparison to their bulk and commercial antibiotics. Interestingly, the combined NPs exhibited promising synergistic biocide efficiency against examined pathogens which encourages their applications in adjuvant therapy and water/wastewater purification for controlling multiple drug-resistant microorganisms. To the best of our knowledge, no previous study reported the synthesis of semiconductor NPs by Proteus mirabilis and the biocide potency of combined NPs against a broad spectrum of pathogens not reported previously. |
url |
http://dx.doi.org/10.1155/2018/5263814 |
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