Proinflammatory and prothrombotic effects on human vascular endothelial cells of Immune-cell-derived LIGHT
<p>Abstract</p> <p>Objective</p> <p>LIGHT (TNFSF 14) belongs to the tumor necrosis factor superfamily and is expressed by activated T cells as well as various types of antigen presenting cells. LIGHT binds to its cellular receptors TR2 and LTßR and has a co-stimulatory...
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doaj-dad36c275c254d36b7df283be73a9eba2020-11-25T00:24:58ZengBMCEuropean Journal of Medical Research2047-783X2009-04-0114414710.1186/2047-783X-14-4-147Proinflammatory and prothrombotic effects on human vascular endothelial cells of Immune-cell-derived LIGHTCelik SShankar VRichter AHippe H-JAkhavanpoor MBea FErbel CUrban SBlank NWambsganss NKatus HADengler TJ<p>Abstract</p> <p>Objective</p> <p>LIGHT (TNFSF 14) belongs to the tumor necrosis factor superfamily and is expressed by activated T cells as well as various types of antigen presenting cells. LIGHT binds to its cellular receptors TR2 and LTßR and has a co-stimulatory role in T cell activation. Here, we compared the relative expression of LIGHT in different immune cells and the biological activity of immune cell-derived LIGHT on endothelial cells.</p> <p>Methods and Results</p> <p>Surface expression of LIGHT and mRNA production by PBMC and isolated T cells (CD4<sup>+ </sup>or CD8<sup>+</sup>) significantly increased after stimulation with PMA (Phorbolester-12-Myristat-13-Acetat) + ionomycin. No LIGHT expression on PMA stimulated monocytes or monocytic-like THP-1 cells could be detected; differentiation of monocytes and THP-1 cells into macrophages, however, resulted in up-regulation of LIGHT. Supernatants of stimulated T cells contained higher concentrations of soluble LIGHT than macrophage supernatants normalized to cell numbers; release of soluble LIGHT was found to be dependent on metalloproteinase activity. Size determination of released soluble LIGHT by size exclusion chromatography revealed a molecular mass of ~60 kDa, suggesting a trimeric form. Released soluble LIGHT induced expression of proinflammatory antigens ICAM-1, tissue factor and IL-8 in human endothelial cells and caused apoptosis of IFN-γ pretreated endothelial cells. Soluble LIGHT was detected at low levels in sera of healthy controls and was significantly enhanced in sera of patients with chronic hepatitis C and rheumatoid arthritis (24.93 ± 9.41 vs.129.53 ± 49.14 and 172.13 ± 77.64; p < 0.0005).</p> <p>Conclusion</p> <p>These findings suggest that among immune cells activated T lymphocytes are the main source of soluble LIGHT with released amounts of soluble LIGHT markedly higher compared to platelets. Immune cell-derived membrane-bound and soluble trimeric LIGHT is biologically active, inducing proinflammatory changes in endothelial cells. Enhanced plasma levels of soluble LIGHT in patients with chronic infections suggest a role of LIGHT in systemic inflammatory activation.</p> http://www.eurjmedres.com/content/14/4/147LIGHTendothelial cellsinflammation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Celik S Shankar V Richter A Hippe H-J Akhavanpoor M Bea F Erbel C Urban S Blank N Wambsganss N Katus HA Dengler TJ |
spellingShingle |
Celik S Shankar V Richter A Hippe H-J Akhavanpoor M Bea F Erbel C Urban S Blank N Wambsganss N Katus HA Dengler TJ Proinflammatory and prothrombotic effects on human vascular endothelial cells of Immune-cell-derived LIGHT European Journal of Medical Research LIGHT endothelial cells inflammation |
author_facet |
Celik S Shankar V Richter A Hippe H-J Akhavanpoor M Bea F Erbel C Urban S Blank N Wambsganss N Katus HA Dengler TJ |
author_sort |
Celik S |
title |
Proinflammatory and prothrombotic effects on human vascular endothelial cells of Immune-cell-derived LIGHT |
title_short |
Proinflammatory and prothrombotic effects on human vascular endothelial cells of Immune-cell-derived LIGHT |
title_full |
Proinflammatory and prothrombotic effects on human vascular endothelial cells of Immune-cell-derived LIGHT |
title_fullStr |
Proinflammatory and prothrombotic effects on human vascular endothelial cells of Immune-cell-derived LIGHT |
title_full_unstemmed |
Proinflammatory and prothrombotic effects on human vascular endothelial cells of Immune-cell-derived LIGHT |
title_sort |
proinflammatory and prothrombotic effects on human vascular endothelial cells of immune-cell-derived light |
publisher |
BMC |
series |
European Journal of Medical Research |
issn |
2047-783X |
publishDate |
2009-04-01 |
description |
<p>Abstract</p> <p>Objective</p> <p>LIGHT (TNFSF 14) belongs to the tumor necrosis factor superfamily and is expressed by activated T cells as well as various types of antigen presenting cells. LIGHT binds to its cellular receptors TR2 and LTßR and has a co-stimulatory role in T cell activation. Here, we compared the relative expression of LIGHT in different immune cells and the biological activity of immune cell-derived LIGHT on endothelial cells.</p> <p>Methods and Results</p> <p>Surface expression of LIGHT and mRNA production by PBMC and isolated T cells (CD4<sup>+ </sup>or CD8<sup>+</sup>) significantly increased after stimulation with PMA (Phorbolester-12-Myristat-13-Acetat) + ionomycin. No LIGHT expression on PMA stimulated monocytes or monocytic-like THP-1 cells could be detected; differentiation of monocytes and THP-1 cells into macrophages, however, resulted in up-regulation of LIGHT. Supernatants of stimulated T cells contained higher concentrations of soluble LIGHT than macrophage supernatants normalized to cell numbers; release of soluble LIGHT was found to be dependent on metalloproteinase activity. Size determination of released soluble LIGHT by size exclusion chromatography revealed a molecular mass of ~60 kDa, suggesting a trimeric form. Released soluble LIGHT induced expression of proinflammatory antigens ICAM-1, tissue factor and IL-8 in human endothelial cells and caused apoptosis of IFN-γ pretreated endothelial cells. Soluble LIGHT was detected at low levels in sera of healthy controls and was significantly enhanced in sera of patients with chronic hepatitis C and rheumatoid arthritis (24.93 ± 9.41 vs.129.53 ± 49.14 and 172.13 ± 77.64; p < 0.0005).</p> <p>Conclusion</p> <p>These findings suggest that among immune cells activated T lymphocytes are the main source of soluble LIGHT with released amounts of soluble LIGHT markedly higher compared to platelets. Immune cell-derived membrane-bound and soluble trimeric LIGHT is biologically active, inducing proinflammatory changes in endothelial cells. Enhanced plasma levels of soluble LIGHT in patients with chronic infections suggest a role of LIGHT in systemic inflammatory activation.</p> |
topic |
LIGHT endothelial cells inflammation |
url |
http://www.eurjmedres.com/content/14/4/147 |
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