Insights into the Bioprospecting of the Endophytic Fungi of the Medicinal Plant <i>Palicourea rigida</i> Kunth (Rubiaceae): Detailed Biological Activities
A multitude of plants from the Brazilian savanna are known for their medicinal properties. Many plants contain endophytic fungi, which lead to the production of bioactive compounds by both the fungi and their hosts. This study investigated the bioprospecting of endophytic fungi recovered from the le...
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Format: | Article |
Language: | English |
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MDPI AG
2021-08-01
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Series: | Journal of Fungi |
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Online Access: | https://www.mdpi.com/2309-608X/7/9/689 |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Igor Romeiro dos Santos Ahmed M. Abdel-Azeem Marwa T. Mohesien Magdalena Piekutowska Donia H. Sheir Lucas Leonardo da Silva Camila da Silva Castro Daniel Diego Costa Carvalho Jadson Diogo Pereira Bezerra Hosam A. Saad Leonardo Luiz Borges Solange Xavier-Santos |
spellingShingle |
Igor Romeiro dos Santos Ahmed M. Abdel-Azeem Marwa T. Mohesien Magdalena Piekutowska Donia H. Sheir Lucas Leonardo da Silva Camila da Silva Castro Daniel Diego Costa Carvalho Jadson Diogo Pereira Bezerra Hosam A. Saad Leonardo Luiz Borges Solange Xavier-Santos Insights into the Bioprospecting of the Endophytic Fungi of the Medicinal Plant <i>Palicourea rigida</i> Kunth (Rubiaceae): Detailed Biological Activities Journal of Fungi endophytic fungi medicinal plants bioprospecting secondary metabolites biotechnological potential natural products |
author_facet |
Igor Romeiro dos Santos Ahmed M. Abdel-Azeem Marwa T. Mohesien Magdalena Piekutowska Donia H. Sheir Lucas Leonardo da Silva Camila da Silva Castro Daniel Diego Costa Carvalho Jadson Diogo Pereira Bezerra Hosam A. Saad Leonardo Luiz Borges Solange Xavier-Santos |
author_sort |
Igor Romeiro dos Santos |
title |
Insights into the Bioprospecting of the Endophytic Fungi of the Medicinal Plant <i>Palicourea rigida</i> Kunth (Rubiaceae): Detailed Biological Activities |
title_short |
Insights into the Bioprospecting of the Endophytic Fungi of the Medicinal Plant <i>Palicourea rigida</i> Kunth (Rubiaceae): Detailed Biological Activities |
title_full |
Insights into the Bioprospecting of the Endophytic Fungi of the Medicinal Plant <i>Palicourea rigida</i> Kunth (Rubiaceae): Detailed Biological Activities |
title_fullStr |
Insights into the Bioprospecting of the Endophytic Fungi of the Medicinal Plant <i>Palicourea rigida</i> Kunth (Rubiaceae): Detailed Biological Activities |
title_full_unstemmed |
Insights into the Bioprospecting of the Endophytic Fungi of the Medicinal Plant <i>Palicourea rigida</i> Kunth (Rubiaceae): Detailed Biological Activities |
title_sort |
insights into the bioprospecting of the endophytic fungi of the medicinal plant <i>palicourea rigida</i> kunth (rubiaceae): detailed biological activities |
publisher |
MDPI AG |
series |
Journal of Fungi |
issn |
2309-608X |
publishDate |
2021-08-01 |
description |
A multitude of plants from the Brazilian savanna are known for their medicinal properties. Many plants contain endophytic fungi, which lead to the production of bioactive compounds by both the fungi and their hosts. This study investigated the bioprospecting of endophytic fungi recovered from the leaves of <i>Palicourea rigida</i>, a native medicinal plant of the Brazilian savanna. Four fungal taxa (<i>Colletotrichum</i> sp. SXS649, <i>Pestalotiopsis</i> sp. SXS650, the order Botryosphaeriales SXS651, and <i>Diaporthe</i> sp. SXS652) were recovered. The phenolic, flavonoid, extracellular degrading enzymes (amylase, cellulase, protease, and tannase) and antioxidant activity of these taxa were determined. Evaluation of the antimicrobial activity showed that the Botryosphaeriales SXS651 extract displays a minimum inhibitory concentration (MIC) of 23.20 mg mL<sup>−1</sup> against <i>Staphylococcus epidermidis</i> and <i>Pseudomonas aeruginosa</i>, and the <i>Diaporthe</i> sp. SXS652 extract exhibited an MIC of 27.00 mg mL<sup>−1</sup> against <i>Escherichia coli</i>. The <i>Colletotrichum</i> sp. SXS649 isolate inhibited tumors in potato discs by 69% at a concentration of 9.70 mg mL<sup>−1</sup>. All isolates had potential bioremediation criteria against soil contaminated with soybean oil, as proved by a high percentage of germination of <i>Lactuca sativa</i> and a reduction in phytotoxicity. Furthermore, the taxa under investigation demonstrated antagonistic action to phytopathogenic fungi, namely, <i>Aspergillus niger</i>, <i>Inonotus rickii</i>, <i>Pestalotiopsis mangiferae</i>, and <i>Coniophora puteana</i>, with an inhibition range between 34.2% and 76.9%. The preliminary toxicity assessment showed that all isolates possessed an LC50 of less than 100 mg mL<sup>−1</sup> to the microcrustacean <i>Artemia salina</i>. These results indicate that the endophytic fungi of the Brazilian savanna are promising candidates for biotechnological and industrial applications and, in agricultural applications, for the biological control of phytopathogenic fungi. |
topic |
endophytic fungi medicinal plants bioprospecting secondary metabolites biotechnological potential natural products |
url |
https://www.mdpi.com/2309-608X/7/9/689 |
work_keys_str_mv |
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doaj-dae333c7a77946c2b6c556d77270730b2021-09-26T00:30:59ZengMDPI AGJournal of Fungi2309-608X2021-08-01768968910.3390/jof7090689Insights into the Bioprospecting of the Endophytic Fungi of the Medicinal Plant <i>Palicourea rigida</i> Kunth (Rubiaceae): Detailed Biological ActivitiesIgor Romeiro dos Santos0Ahmed M. Abdel-Azeem1Marwa T. Mohesien2Magdalena Piekutowska3Donia H. Sheir4Lucas Leonardo da Silva5Camila da Silva Castro6Daniel Diego Costa Carvalho7Jadson Diogo Pereira Bezerra8Hosam A. Saad9Leonardo Luiz Borges10Solange Xavier-Santos11Basic, Applied and Scientific Divulgation Mycolgy Laboratory (FungiLab), Central Campus, State University of Goiás, Anápolis 75132-903, GO, BrazilBotany and Microbiology Department, Faculty of Science, Suez Canal University, Ismailia 41522, EgyptBotany and Microbiology Department, Faculty of Science, Damietta University, New Damietta 34511, EgyptDepartment of Geoecology and Geoinformation, Institute of Biology and Earth Sciences, Pomeranian University in Słupsk, Partyzantów 27, 76-200 Słupsk, PolandNational Research Centre, Chemistry of Natural and Microbial Products Department, Pharmaceutical Industries Division, Giza 12622, EgyptBasic, Applied and Scientific Divulgation Mycolgy Laboratory (FungiLab), Central Campus, State University of Goiás, Anápolis 75132-903, GO, BrazilBasic, Applied and Scientific Divulgation Mycolgy Laboratory (FungiLab), Central Campus, State University of Goiás, Anápolis 75132-903, GO, BrazilPhytopathology Laboratory, Southeast Campus, State University of Goiás, Ipameri 75780-000, GO, BrazilMycology Sector, Department of Biosciences and Technology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia 74690-900, GO, BrazilDepartment of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaBasic, Applied and Scientific Divulgation Mycolgy Laboratory (FungiLab), Central Campus, State University of Goiás, Anápolis 75132-903, GO, BrazilBotany and Microbiology Department, Faculty of Science, Suez Canal University, Ismailia 41522, EgyptA multitude of plants from the Brazilian savanna are known for their medicinal properties. Many plants contain endophytic fungi, which lead to the production of bioactive compounds by both the fungi and their hosts. This study investigated the bioprospecting of endophytic fungi recovered from the leaves of <i>Palicourea rigida</i>, a native medicinal plant of the Brazilian savanna. Four fungal taxa (<i>Colletotrichum</i> sp. SXS649, <i>Pestalotiopsis</i> sp. SXS650, the order Botryosphaeriales SXS651, and <i>Diaporthe</i> sp. SXS652) were recovered. The phenolic, flavonoid, extracellular degrading enzymes (amylase, cellulase, protease, and tannase) and antioxidant activity of these taxa were determined. Evaluation of the antimicrobial activity showed that the Botryosphaeriales SXS651 extract displays a minimum inhibitory concentration (MIC) of 23.20 mg mL<sup>−1</sup> against <i>Staphylococcus epidermidis</i> and <i>Pseudomonas aeruginosa</i>, and the <i>Diaporthe</i> sp. SXS652 extract exhibited an MIC of 27.00 mg mL<sup>−1</sup> against <i>Escherichia coli</i>. The <i>Colletotrichum</i> sp. SXS649 isolate inhibited tumors in potato discs by 69% at a concentration of 9.70 mg mL<sup>−1</sup>. All isolates had potential bioremediation criteria against soil contaminated with soybean oil, as proved by a high percentage of germination of <i>Lactuca sativa</i> and a reduction in phytotoxicity. Furthermore, the taxa under investigation demonstrated antagonistic action to phytopathogenic fungi, namely, <i>Aspergillus niger</i>, <i>Inonotus rickii</i>, <i>Pestalotiopsis mangiferae</i>, and <i>Coniophora puteana</i>, with an inhibition range between 34.2% and 76.9%. The preliminary toxicity assessment showed that all isolates possessed an LC50 of less than 100 mg mL<sup>−1</sup> to the microcrustacean <i>Artemia salina</i>. These results indicate that the endophytic fungi of the Brazilian savanna are promising candidates for biotechnological and industrial applications and, in agricultural applications, for the biological control of phytopathogenic fungi.https://www.mdpi.com/2309-608X/7/9/689endophytic fungimedicinal plantsbioprospectingsecondary metabolitesbiotechnological potentialnatural products |