Chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity of Convolvulus pluricaulis
Herbal medicines are known to mitigate radical induced cell damage. Hence identification and scientific validation of herbal medicines contribute to better use in Ayurvedic/Unani research. In the present study, we investigated antioxidant and anti-apoptotic properties of Convolvulus pluricaulis (C. ...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2018-10-01
|
Series: | Journal of Traditional and Complementary Medicine |
Online Access: | http://www.sciencedirect.com/science/article/pii/S222541101730130X |
id |
doaj-daedb7d528e24aacb4114b365dd350ee |
---|---|
record_format |
Article |
spelling |
doaj-daedb7d528e24aacb4114b365dd350ee2020-11-24T22:11:52ZengElsevierJournal of Traditional and Complementary Medicine2225-41102018-10-0184483496Chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity of Convolvulus pluricaulisP. Rachitha0K. Krupashree1G.V. Jayashree2Hemanth Kumar Kandikattu3Narayanappa Amruta4Natarajan Gopalan5M.K. Rao6Farhath Khanum7Biochemistry and Nanosciences Discipline, Defence Food Research Laboratory, Mysuru, IndiaBiochemistry and Nanosciences Discipline, Defence Food Research Laboratory, Mysuru, IndiaBiochemistry and Nanosciences Discipline, Defence Food Research Laboratory, Mysuru, IndiaBiochemistry and Nanosciences Discipline, Defence Food Research Laboratory, Mysuru, IndiaDepartment of Seed Science and Technology, UAS, Bengaluru, IndiaFood Biotechnology, Defence Food Research Laboratory, Mysuru, IndiaDepartment of Toxicology, Defence Research and Development Establishment, Gwalior, IndiaBiochemistry and Nanosciences Discipline, Defence Food Research Laboratory, Mysuru, India; Corresponding author. Biochemistry and Nanosciences Discipline, Defence Food Research Laboratory, Mysuru, India.Herbal medicines are known to mitigate radical induced cell damage. Hence identification and scientific validation of herbal medicines contribute to better use in Ayurvedic/Unani research. In the present study, we investigated antioxidant and anti-apoptotic properties of Convolvulus pluricaulis (C. pluricaulis). C. pluricaulis exhibited antioxidant potential evident by free radical scavenging activities. C. pluricaulis pretreatment inhibited H2O2 induced macromolecule damage such as plasmid DNA damage and AAPH induced oxidation of bovine serum albumin and lipid peroxidation of rat hepatic tissues. Further to identify the neuroprotective properties of C. pluricaulis, SHSY5Y cells were treated with H2O2 with or without pretreatment of C. pluricaulis. The C. pluricaulis pretreatment at 50 μg/ml dose exhibited 50% cell survival against 100 μM H2O2 challenge for 24 h and it also decreased the lactate dehydrogenase leakage. Further C. pluricaulis pretreatment restored and regulated the antioxidant and apoptosis markers such as SOD, CAT, p53, and caspase-3 and inhibited, reactive oxygen species generation and depolarization of the mitochondrial membrane. C. pluricaulis possess a high content of flavonoids and polyphenols and GC-MS and FTIR analysis showed a wide variety of compounds which may contribute to the observed effects. Keywords: Convolvulus pluricaulis, GC-MS, FTIR, Antioxidant, SH-SY5Y, Neuroprotectionhttp://www.sciencedirect.com/science/article/pii/S222541101730130X |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
P. Rachitha K. Krupashree G.V. Jayashree Hemanth Kumar Kandikattu Narayanappa Amruta Natarajan Gopalan M.K. Rao Farhath Khanum |
spellingShingle |
P. Rachitha K. Krupashree G.V. Jayashree Hemanth Kumar Kandikattu Narayanappa Amruta Natarajan Gopalan M.K. Rao Farhath Khanum Chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity of Convolvulus pluricaulis Journal of Traditional and Complementary Medicine |
author_facet |
P. Rachitha K. Krupashree G.V. Jayashree Hemanth Kumar Kandikattu Narayanappa Amruta Natarajan Gopalan M.K. Rao Farhath Khanum |
author_sort |
P. Rachitha |
title |
Chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity of Convolvulus pluricaulis |
title_short |
Chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity of Convolvulus pluricaulis |
title_full |
Chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity of Convolvulus pluricaulis |
title_fullStr |
Chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity of Convolvulus pluricaulis |
title_full_unstemmed |
Chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity of Convolvulus pluricaulis |
title_sort |
chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity of convolvulus pluricaulis |
publisher |
Elsevier |
series |
Journal of Traditional and Complementary Medicine |
issn |
2225-4110 |
publishDate |
2018-10-01 |
description |
Herbal medicines are known to mitigate radical induced cell damage. Hence identification and scientific validation of herbal medicines contribute to better use in Ayurvedic/Unani research. In the present study, we investigated antioxidant and anti-apoptotic properties of Convolvulus pluricaulis (C. pluricaulis). C. pluricaulis exhibited antioxidant potential evident by free radical scavenging activities. C. pluricaulis pretreatment inhibited H2O2 induced macromolecule damage such as plasmid DNA damage and AAPH induced oxidation of bovine serum albumin and lipid peroxidation of rat hepatic tissues. Further to identify the neuroprotective properties of C. pluricaulis, SHSY5Y cells were treated with H2O2 with or without pretreatment of C. pluricaulis. The C. pluricaulis pretreatment at 50 μg/ml dose exhibited 50% cell survival against 100 μM H2O2 challenge for 24 h and it also decreased the lactate dehydrogenase leakage. Further C. pluricaulis pretreatment restored and regulated the antioxidant and apoptosis markers such as SOD, CAT, p53, and caspase-3 and inhibited, reactive oxygen species generation and depolarization of the mitochondrial membrane. C. pluricaulis possess a high content of flavonoids and polyphenols and GC-MS and FTIR analysis showed a wide variety of compounds which may contribute to the observed effects. Keywords: Convolvulus pluricaulis, GC-MS, FTIR, Antioxidant, SH-SY5Y, Neuroprotection |
url |
http://www.sciencedirect.com/science/article/pii/S222541101730130X |
work_keys_str_mv |
AT prachitha chemicalcompositionantioxidantpotentialmacromoleculedamageandneuroprotectiveactivityofconvolvuluspluricaulis AT kkrupashree chemicalcompositionantioxidantpotentialmacromoleculedamageandneuroprotectiveactivityofconvolvuluspluricaulis AT gvjayashree chemicalcompositionantioxidantpotentialmacromoleculedamageandneuroprotectiveactivityofconvolvuluspluricaulis AT hemanthkumarkandikattu chemicalcompositionantioxidantpotentialmacromoleculedamageandneuroprotectiveactivityofconvolvuluspluricaulis AT narayanappaamruta chemicalcompositionantioxidantpotentialmacromoleculedamageandneuroprotectiveactivityofconvolvuluspluricaulis AT natarajangopalan chemicalcompositionantioxidantpotentialmacromoleculedamageandneuroprotectiveactivityofconvolvuluspluricaulis AT mkrao chemicalcompositionantioxidantpotentialmacromoleculedamageandneuroprotectiveactivityofconvolvuluspluricaulis AT farhathkhanum chemicalcompositionantioxidantpotentialmacromoleculedamageandneuroprotectiveactivityofconvolvuluspluricaulis |
_version_ |
1725803866048430080 |