High expression of anti-apoptotic genes in grade I and II meningiomas

• Main Authors: Daniela Pretti da Cunha Tirapelli, Sarah Bomfim Menezes, Indira Maynart Franco, Isis Lacrose Lustosa, Andressa Romualdo Rodrigues, Paulo Cézar Novais, Antônio César Mendes Santiago, Fernanda Maris Peria, Luciano Neder Serafini, Alexandre Magno da Nóbrega Marinho, Carlos Gilberto Carlotti Jr, Benedicto Oscar Colli, Luís Fernando Tirapelli
• Format: Article
• Language: English
• Published: Academia Brasileira de Neurologia (ABNEURO)
• Series: Arquivos de Neuro-Psiquiatria
• Subjects: meningioma; carcinogênese; apoptose; expressão gênica
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2017000400209&lng=en&tlng=en

ABSTRACT One of the different genetic mechanisms involved in the carcinogenesis of meningiomas is influenced by interactions between proteins that induce and inhibit apoptosis. Objective To evaluate the expression of c-FLIP, XIAP, Bcl-2, caspase 3, 8 and 9, cytochrome c, APAF 1 and Smac/DIABLO genes related to apoptosis pathways. Methods The gene expression was evaluated in 30 meningiomas (WHO grades I and II) and in 10 normal samples (from arachnoid tissue) through PCR-RT. Results The results showed higher expression of anti-apoptotic genes in meningiomas when compared to the control group, which had a low expression of pro-apoptotic genes. Conclusion There is a possible block in the activation of caspases through the intrinsic apoptosis pathway in meningiomas. c-FLIP modulates caspase 8 and, by inhibiting its activation due to the lack of connection with the receiver, there is a block to the FAS activation of apoptosis by its extrinsic pathway.