Physicochemical Properties of Bovine Serum Albumin-Glucose and Bovine Serum Albumin-Mannose Conjugates Prepared by Pulsed Electric Fields Treatment
The pulsed electric fields (PEF) treatment is a novel method for obtaining glycated proteins by way of a Maillard reaction between proteins and polysaccharides but its effect on the preparation of protein–monosaccharide conjugate has not been explored. This study aimed to prepare bovine serum albumi...
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doaj-db0e3cc2e2614f0f868dd335405699902020-11-24T23:02:33ZengMDPI AGMolecules1420-30492018-03-0123357010.3390/molecules23030570molecules23030570Physicochemical Properties of Bovine Serum Albumin-Glucose and Bovine Serum Albumin-Mannose Conjugates Prepared by Pulsed Electric Fields TreatmentWenjie Jian0Liangyu Wang1Lanlan Wu2Yuan-ming Sun3Institute of Nutrition and food Safety, Xiamen Medical College, Xiamen 361023, ChinaDepartment of Biology and Chemistry Engineering, Fuqing Branch of Fujian Normal University, Fuzhou 50300, ChinaInstitute of Nutrition and food Safety, Xiamen Medical College, Xiamen 361023, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaThe pulsed electric fields (PEF) treatment is a novel method for obtaining glycated proteins by way of a Maillard reaction between proteins and polysaccharides but its effect on the preparation of protein–monosaccharide conjugate has not been explored. This study aimed to prepare bovine serum albumin (BSA)–glucose and BSA–mannose conjugates using PEF in pH 10.0 at an intensity of 10 or 20 kV/cm, frequency of 1 kHz, pulse width of 20 μs and 73.5 pulses. The conjugates were evaluated for physicochemical properties. The results indicated that PEF not only promoted Maillard reaction between BSA and glucose or mannose but also alleviated the undesirable browning. PEF treatment favored the increased surface hydrophobicity and emulsifying activity in BSA but reduced surface hydrophobicity and foaming stability and improved foaming capacity in BSA–glucose and BSA–mannose conjugates. These findings provided useful considerations in the application of PEF treatment as a potential method to prepare BSA–monosaccharide conjugates by Maillard reaction.http://www.mdpi.com/1420-3049/23/3/570bovine serum albuminglucosemannosephysicochemical propertiespulsed electric fields treatment |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wenjie Jian Liangyu Wang Lanlan Wu Yuan-ming Sun |
spellingShingle |
Wenjie Jian Liangyu Wang Lanlan Wu Yuan-ming Sun Physicochemical Properties of Bovine Serum Albumin-Glucose and Bovine Serum Albumin-Mannose Conjugates Prepared by Pulsed Electric Fields Treatment Molecules bovine serum albumin glucose mannose physicochemical properties pulsed electric fields treatment |
author_facet |
Wenjie Jian Liangyu Wang Lanlan Wu Yuan-ming Sun |
author_sort |
Wenjie Jian |
title |
Physicochemical Properties of Bovine Serum Albumin-Glucose and Bovine Serum Albumin-Mannose Conjugates Prepared by Pulsed Electric Fields Treatment |
title_short |
Physicochemical Properties of Bovine Serum Albumin-Glucose and Bovine Serum Albumin-Mannose Conjugates Prepared by Pulsed Electric Fields Treatment |
title_full |
Physicochemical Properties of Bovine Serum Albumin-Glucose and Bovine Serum Albumin-Mannose Conjugates Prepared by Pulsed Electric Fields Treatment |
title_fullStr |
Physicochemical Properties of Bovine Serum Albumin-Glucose and Bovine Serum Albumin-Mannose Conjugates Prepared by Pulsed Electric Fields Treatment |
title_full_unstemmed |
Physicochemical Properties of Bovine Serum Albumin-Glucose and Bovine Serum Albumin-Mannose Conjugates Prepared by Pulsed Electric Fields Treatment |
title_sort |
physicochemical properties of bovine serum albumin-glucose and bovine serum albumin-mannose conjugates prepared by pulsed electric fields treatment |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-03-01 |
description |
The pulsed electric fields (PEF) treatment is a novel method for obtaining glycated proteins by way of a Maillard reaction between proteins and polysaccharides but its effect on the preparation of protein–monosaccharide conjugate has not been explored. This study aimed to prepare bovine serum albumin (BSA)–glucose and BSA–mannose conjugates using PEF in pH 10.0 at an intensity of 10 or 20 kV/cm, frequency of 1 kHz, pulse width of 20 μs and 73.5 pulses. The conjugates were evaluated for physicochemical properties. The results indicated that PEF not only promoted Maillard reaction between BSA and glucose or mannose but also alleviated the undesirable browning. PEF treatment favored the increased surface hydrophobicity and emulsifying activity in BSA but reduced surface hydrophobicity and foaming stability and improved foaming capacity in BSA–glucose and BSA–mannose conjugates. These findings provided useful considerations in the application of PEF treatment as a potential method to prepare BSA–monosaccharide conjugates by Maillard reaction. |
topic |
bovine serum albumin glucose mannose physicochemical properties pulsed electric fields treatment |
url |
http://www.mdpi.com/1420-3049/23/3/570 |
work_keys_str_mv |
AT wenjiejian physicochemicalpropertiesofbovineserumalbuminglucoseandbovineserumalbuminmannoseconjugatespreparedbypulsedelectricfieldstreatment AT liangyuwang physicochemicalpropertiesofbovineserumalbuminglucoseandbovineserumalbuminmannoseconjugatespreparedbypulsedelectricfieldstreatment AT lanlanwu physicochemicalpropertiesofbovineserumalbuminglucoseandbovineserumalbuminmannoseconjugatespreparedbypulsedelectricfieldstreatment AT yuanmingsun physicochemicalpropertiesofbovineserumalbuminglucoseandbovineserumalbuminmannoseconjugatespreparedbypulsedelectricfieldstreatment |
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1725636304349167616 |