DJ-1 Inhibits α-Synuclein Aggregation by Regulating Chaperone-Mediated Autophagy
α-Synuclein misfolding and aggregation play an important role in the pathogenesis of Parkinson’s disease (PD). Loss of function and mutation of the PARK7/DJ-1 gene cause early-onset familial PD. DJ-1 can inhibit α-synuclein aggregation, and may function at an early step in the aggregation process. S...
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doaj-db2f3e9fccec49a886b20403b9ef7acd2020-11-24T22:39:33ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652017-09-01910.3389/fnagi.2017.00308282462DJ-1 Inhibits α-Synuclein Aggregation by Regulating Chaperone-Mediated AutophagyChuan-Ying Xu0Wen-Yan Kang1Yi-Meng Chen2Tian-Fang Jiang3Jia Zhang4Li-Na Zhang5Jian-Qing Ding6Jun Liu7Sheng-Di Chen8Sheng-Di Chen9Department of Neurology and Collaborative Innovation Center for Brain Science, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Neurology and Collaborative Innovation Center for Brain Science, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaLaboratory of Neurodegenerative Diseases, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, ChinaDepartment of Neurology and Collaborative Innovation Center for Brain Science, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Neurology and Collaborative Innovation Center for Brain Science, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Biostatistics, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Neurology and Collaborative Innovation Center for Brain Science, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Neurology and Collaborative Innovation Center for Brain Science, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Neurology and Collaborative Innovation Center for Brain Science, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaLaboratory of Neurodegenerative Diseases, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, Chinaα-Synuclein misfolding and aggregation play an important role in the pathogenesis of Parkinson’s disease (PD). Loss of function and mutation of the PARK7/DJ-1 gene cause early-onset familial PD. DJ-1 can inhibit α-synuclein aggregation, and may function at an early step in the aggregation process. Soluble wild-type (WT) α-synuclein is mainly degraded by chaperone-mediated autophagy (CMA), and impairment of CMA is closely related to the pathogenesis of PD. Here, we investigated whether DJ-1 could reduce α-synuclein accumulation and aggregation by CMA. DJ-1 knockout mice and DJ-1 siRNA knockdown SH-SY5Y cells were used to investigate the potential mechanisms underlying the relationship between DJ-1 deficiency and α-synuclein aggregation. First, we confirmed that DJ-1 deficiency increased the accumulation and aggregation of α-synuclein in both SH-SY5Y cells and PD animal models, and overexpression of DJ-1 in vitro effectively decreased α-synuclein levels. α-Synuclein overexpression activated CMA by elevating the levels of lysosome-associated membrane protein type-2A (LAMP2A), but DJ-1 deficiency suppressed upregulation of LAMP2A. DJ-1 deficiency downregulated the level of lysosomal 70 kDa heat-shock cognate protein (HSC70) but not the levels of that in homogenates. Further studies showed that DJ-1 deficiency accelerated the degradation of LAMP2A in lysosomes, leading to the aggregation of α-synuclein. Our study suggests that DJ-1 deficiency aggravates α-synuclein aggregation by inhibiting the activation of CMA and provides further evidence of the molecular interaction between PD-related proteins via the CMA pathway.http://journal.frontiersin.org/article/10.3389/fnagi.2017.00308/fullParkinson’s diseaseα-synucleinDJ-1chaperone-mediated autophagyLAMP2A |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chuan-Ying Xu Wen-Yan Kang Yi-Meng Chen Tian-Fang Jiang Jia Zhang Li-Na Zhang Jian-Qing Ding Jun Liu Sheng-Di Chen Sheng-Di Chen |
spellingShingle |
Chuan-Ying Xu Wen-Yan Kang Yi-Meng Chen Tian-Fang Jiang Jia Zhang Li-Na Zhang Jian-Qing Ding Jun Liu Sheng-Di Chen Sheng-Di Chen DJ-1 Inhibits α-Synuclein Aggregation by Regulating Chaperone-Mediated Autophagy Frontiers in Aging Neuroscience Parkinson’s disease α-synuclein DJ-1 chaperone-mediated autophagy LAMP2A |
author_facet |
Chuan-Ying Xu Wen-Yan Kang Yi-Meng Chen Tian-Fang Jiang Jia Zhang Li-Na Zhang Jian-Qing Ding Jun Liu Sheng-Di Chen Sheng-Di Chen |
author_sort |
Chuan-Ying Xu |
title |
DJ-1 Inhibits α-Synuclein Aggregation by Regulating Chaperone-Mediated Autophagy |
title_short |
DJ-1 Inhibits α-Synuclein Aggregation by Regulating Chaperone-Mediated Autophagy |
title_full |
DJ-1 Inhibits α-Synuclein Aggregation by Regulating Chaperone-Mediated Autophagy |
title_fullStr |
DJ-1 Inhibits α-Synuclein Aggregation by Regulating Chaperone-Mediated Autophagy |
title_full_unstemmed |
DJ-1 Inhibits α-Synuclein Aggregation by Regulating Chaperone-Mediated Autophagy |
title_sort |
dj-1 inhibits α-synuclein aggregation by regulating chaperone-mediated autophagy |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Aging Neuroscience |
issn |
1663-4365 |
publishDate |
2017-09-01 |
description |
α-Synuclein misfolding and aggregation play an important role in the pathogenesis of Parkinson’s disease (PD). Loss of function and mutation of the PARK7/DJ-1 gene cause early-onset familial PD. DJ-1 can inhibit α-synuclein aggregation, and may function at an early step in the aggregation process. Soluble wild-type (WT) α-synuclein is mainly degraded by chaperone-mediated autophagy (CMA), and impairment of CMA is closely related to the pathogenesis of PD. Here, we investigated whether DJ-1 could reduce α-synuclein accumulation and aggregation by CMA. DJ-1 knockout mice and DJ-1 siRNA knockdown SH-SY5Y cells were used to investigate the potential mechanisms underlying the relationship between DJ-1 deficiency and α-synuclein aggregation. First, we confirmed that DJ-1 deficiency increased the accumulation and aggregation of α-synuclein in both SH-SY5Y cells and PD animal models, and overexpression of DJ-1 in vitro effectively decreased α-synuclein levels. α-Synuclein overexpression activated CMA by elevating the levels of lysosome-associated membrane protein type-2A (LAMP2A), but DJ-1 deficiency suppressed upregulation of LAMP2A. DJ-1 deficiency downregulated the level of lysosomal 70 kDa heat-shock cognate protein (HSC70) but not the levels of that in homogenates. Further studies showed that DJ-1 deficiency accelerated the degradation of LAMP2A in lysosomes, leading to the aggregation of α-synuclein. Our study suggests that DJ-1 deficiency aggravates α-synuclein aggregation by inhibiting the activation of CMA and provides further evidence of the molecular interaction between PD-related proteins via the CMA pathway. |
topic |
Parkinson’s disease α-synuclein DJ-1 chaperone-mediated autophagy LAMP2A |
url |
http://journal.frontiersin.org/article/10.3389/fnagi.2017.00308/full |
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