In vitro biomechanical properties, fluorescence imaging, surface-enhanced Raman spectroscopy, and photothermal therapy evaluation of luminescent functionalized CaMoO4:Eu@Au hybrid nanorods on human lung adenocarcinoma epithelial cells
Highly dispersible Eu3+-doped CaMoO4@Au-nanorod hybrid nanoparticles (HNPs) exhibit optical properties, such as plasmon resonances in the near-infrared region at 790 nm and luminescence at 615 nm, offering multimodal capabilities: fluorescence imaging, surface-enhanced Raman spectroscopy (SERS) dete...
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doaj-db5507f1f75f4201aea77a5d61ecab5e2021-07-06T11:30:13ZengTaylor & Francis GroupScience and Technology of Advanced Materials1468-69961878-55142016-01-0117134636010.1080/14686996.2016.11897971189797In vitro biomechanical properties, fluorescence imaging, surface-enhanced Raman spectroscopy, and photothermal therapy evaluation of luminescent functionalized CaMoO4:Eu@Au hybrid nanorods on human lung adenocarcinoma epithelial cellsQifei Li0Abdul K. Parchur1Anhong Zhou2Department of Biological Engineering, Utah State UniversityDepartment of Biological Engineering, Utah State UniversityDepartment of Biological Engineering, Utah State UniversityHighly dispersible Eu3+-doped CaMoO4@Au-nanorod hybrid nanoparticles (HNPs) exhibit optical properties, such as plasmon resonances in the near-infrared region at 790 nm and luminescence at 615 nm, offering multimodal capabilities: fluorescence imaging, surface-enhanced Raman spectroscopy (SERS) detection and photothermal therapy (PTT). HNPs were conjugated with a Raman reporter (4-mercaptobenzoic acid), showing a desired SERS signal (enhancement factor 5.0 × 105). The HNPs have a heat conversion efficiency of 25.6%, and a hyperthermia temperature of 42°C could be achieved by adjusting either concentration of HNPs, or laser power, or irradiation time. HNPs were modified with antibody specific to cancer biomarker epidermal growth factor receptor, then applied to human lung cancer (A549) and mouse hepatocyte cells (AML12), and in vitro PTT effect was studied. In addition, the biomechanical properties of A549 cells were quantified using atomic force microscopy. This study shows the potential applications of these HNPs in fluorescence imaging, SERS detection, and PTT with good photostability and biocompatibility.http://dx.doi.org/10.1080/14686996.2016.1189797luminescent functionalized hybrid nanoparticlesbiomechanical propertiessurface-enhanced raman spectroscopy (sers)photothermal therapy (ptt)multi-modal imaging probe |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qifei Li Abdul K. Parchur Anhong Zhou |
spellingShingle |
Qifei Li Abdul K. Parchur Anhong Zhou In vitro biomechanical properties, fluorescence imaging, surface-enhanced Raman spectroscopy, and photothermal therapy evaluation of luminescent functionalized CaMoO4:Eu@Au hybrid nanorods on human lung adenocarcinoma epithelial cells Science and Technology of Advanced Materials luminescent functionalized hybrid nanoparticles biomechanical properties surface-enhanced raman spectroscopy (sers) photothermal therapy (ptt) multi-modal imaging probe |
author_facet |
Qifei Li Abdul K. Parchur Anhong Zhou |
author_sort |
Qifei Li |
title |
In vitro biomechanical properties, fluorescence imaging, surface-enhanced Raman spectroscopy, and photothermal therapy evaluation of luminescent functionalized CaMoO4:Eu@Au hybrid nanorods on human lung adenocarcinoma epithelial cells |
title_short |
In vitro biomechanical properties, fluorescence imaging, surface-enhanced Raman spectroscopy, and photothermal therapy evaluation of luminescent functionalized CaMoO4:Eu@Au hybrid nanorods on human lung adenocarcinoma epithelial cells |
title_full |
In vitro biomechanical properties, fluorescence imaging, surface-enhanced Raman spectroscopy, and photothermal therapy evaluation of luminescent functionalized CaMoO4:Eu@Au hybrid nanorods on human lung adenocarcinoma epithelial cells |
title_fullStr |
In vitro biomechanical properties, fluorescence imaging, surface-enhanced Raman spectroscopy, and photothermal therapy evaluation of luminescent functionalized CaMoO4:Eu@Au hybrid nanorods on human lung adenocarcinoma epithelial cells |
title_full_unstemmed |
In vitro biomechanical properties, fluorescence imaging, surface-enhanced Raman spectroscopy, and photothermal therapy evaluation of luminescent functionalized CaMoO4:Eu@Au hybrid nanorods on human lung adenocarcinoma epithelial cells |
title_sort |
in vitro biomechanical properties, fluorescence imaging, surface-enhanced raman spectroscopy, and photothermal therapy evaluation of luminescent functionalized camoo4:eu@au hybrid nanorods on human lung adenocarcinoma epithelial cells |
publisher |
Taylor & Francis Group |
series |
Science and Technology of Advanced Materials |
issn |
1468-6996 1878-5514 |
publishDate |
2016-01-01 |
description |
Highly dispersible Eu3+-doped CaMoO4@Au-nanorod hybrid nanoparticles (HNPs) exhibit optical properties, such as plasmon resonances in the near-infrared region at 790 nm and luminescence at 615 nm, offering multimodal capabilities: fluorescence imaging, surface-enhanced Raman spectroscopy (SERS) detection and photothermal therapy (PTT). HNPs were conjugated with a Raman reporter (4-mercaptobenzoic acid), showing a desired SERS signal (enhancement factor 5.0 × 105). The HNPs have a heat conversion efficiency of 25.6%, and a hyperthermia temperature of 42°C could be achieved by adjusting either concentration of HNPs, or laser power, or irradiation time. HNPs were modified with antibody specific to cancer biomarker epidermal growth factor receptor, then applied to human lung cancer (A549) and mouse hepatocyte cells (AML12), and in vitro PTT effect was studied. In addition, the biomechanical properties of A549 cells were quantified using atomic force microscopy. This study shows the potential applications of these HNPs in fluorescence imaging, SERS detection, and PTT with good photostability and biocompatibility. |
topic |
luminescent functionalized hybrid nanoparticles biomechanical properties surface-enhanced raman spectroscopy (sers) photothermal therapy (ptt) multi-modal imaging probe |
url |
http://dx.doi.org/10.1080/14686996.2016.1189797 |
work_keys_str_mv |
AT qifeili invitrobiomechanicalpropertiesfluorescenceimagingsurfaceenhancedramanspectroscopyandphotothermaltherapyevaluationofluminescentfunctionalizedcamoo4euauhybridnanorodsonhumanlungadenocarcinomaepithelialcells AT abdulkparchur invitrobiomechanicalpropertiesfluorescenceimagingsurfaceenhancedramanspectroscopyandphotothermaltherapyevaluationofluminescentfunctionalizedcamoo4euauhybridnanorodsonhumanlungadenocarcinomaepithelialcells AT anhongzhou invitrobiomechanicalpropertiesfluorescenceimagingsurfaceenhancedramanspectroscopyandphotothermaltherapyevaluationofluminescentfunctionalizedcamoo4euauhybridnanorodsonhumanlungadenocarcinomaepithelialcells |
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1721317584177987584 |