Protective effect of apigenin magnesium complex on H2O2-induced oxidative stress and inflammatory responses in rat hepatic stellate cells
Context Apigenin displays antioxidant and anti-inflammatory effects. However, effects of apigenin magnesium (AM) complex on these aspects remain unknown. Objective This study investigated the effects of AM complex on oxidative stress and inflammatory responses in hydrogen peroxide (H2O2)-induced rat...
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doaj-db684a3f946d4847b227c796a32bbc2e2021-05-06T15:44:47ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162020-01-0158155356010.1080/13880209.2020.17728401772840Protective effect of apigenin magnesium complex on H2O2-induced oxidative stress and inflammatory responses in rat hepatic stellate cellsXuwang Pan0Yidan Shao1Fugen Wang2Zhaobin Cai3Shourong Liu4Jianjun Xi5Ruoyu He6Yanmei Zhao7Rangxiao Zhuang8Department of Pharmaceutical Preparation, Hangzhou Xixi HospitalDepartment of Pharmaceutical Preparation, Hangzhou Xixi HospitalDepartment of Pharmaceutical Preparation, Hangzhou Xixi HospitalDepartment of Liver Disease, Hangzhou Xixi HospitalDepartment of Liver Disease, Hangzhou Xixi HospitalDepartment of Pharmaceutical Preparation, Hangzhou Xixi HospitalDepartment of Pharmaceutical Preparation, Hangzhou Xixi HospitalDepartment of Pharmaceutical Preparation, Hangzhou Xixi HospitalDepartment of Pharmaceutical Preparation, Hangzhou Xixi HospitalContext Apigenin displays antioxidant and anti-inflammatory effects. However, effects of apigenin magnesium (AM) complex on these aspects remain unknown. Objective This study investigated the effects of AM complex on oxidative stress and inflammatory responses in hydrogen peroxide (H2O2)-induced rat hepatic stellate cells (HSCs). Materials and methods The antioxidant and anti-inflammatory effects of AM complex at concentrations of 0.625, 1.25, and 2.5 mg/mL were evaluated, comparing to HSCs treated by H2O2 alone. Cell viability, reactive oxygen species (ROS), the activity of superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), nitric oxide (NO), interleukin 6 (IL-6), and nuclear factor-kappa B (NF-κB) levels were measured. Moreover, cell apoptosis, mRNA expression levels of transforming growth factor-β (TGF-β), NF-κB, and inducible nitric oxide synthase (iNOS) were assessed. Results AM complex significantly inhibited oxidative stress and inflammatory response at concentrations of 0.625, 1.25, and 2.5 mg/mL (IC50 = 1.679 mg/mL). AM complex elevated the survival rate of H2O2-treated HSCs and had no toxic effects on HSCs. AM complex also promoted SOD activity and GSH levels but suppressed ROS, MDA, and NO levels. Additionally, AM complex decreased IL-6 and NF-κB levels, gene expression of TGF-β, NF-κB, and iNOS, as well as induced apoptosis of HSCs. Discussion and conclusions Data indicated that AM complex mitigated oxidative stress and inflammatory responses on H2O2-treated HSCs, suggesting that AM complex is a possible candidate for anti-hepatic diseases. Additional efforts, both in vivo and in humans, are required to assess of AM complex as a potential therapeutic drug in liver diseases.http://dx.doi.org/10.1080/13880209.2020.1772840mttredox systemproinflammatory cytokinesreal-time pcr |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xuwang Pan Yidan Shao Fugen Wang Zhaobin Cai Shourong Liu Jianjun Xi Ruoyu He Yanmei Zhao Rangxiao Zhuang |
spellingShingle |
Xuwang Pan Yidan Shao Fugen Wang Zhaobin Cai Shourong Liu Jianjun Xi Ruoyu He Yanmei Zhao Rangxiao Zhuang Protective effect of apigenin magnesium complex on H2O2-induced oxidative stress and inflammatory responses in rat hepatic stellate cells Pharmaceutical Biology mtt redox system proinflammatory cytokines real-time pcr |
author_facet |
Xuwang Pan Yidan Shao Fugen Wang Zhaobin Cai Shourong Liu Jianjun Xi Ruoyu He Yanmei Zhao Rangxiao Zhuang |
author_sort |
Xuwang Pan |
title |
Protective effect of apigenin magnesium complex on H2O2-induced oxidative stress and inflammatory responses in rat hepatic stellate cells |
title_short |
Protective effect of apigenin magnesium complex on H2O2-induced oxidative stress and inflammatory responses in rat hepatic stellate cells |
title_full |
Protective effect of apigenin magnesium complex on H2O2-induced oxidative stress and inflammatory responses in rat hepatic stellate cells |
title_fullStr |
Protective effect of apigenin magnesium complex on H2O2-induced oxidative stress and inflammatory responses in rat hepatic stellate cells |
title_full_unstemmed |
Protective effect of apigenin magnesium complex on H2O2-induced oxidative stress and inflammatory responses in rat hepatic stellate cells |
title_sort |
protective effect of apigenin magnesium complex on h2o2-induced oxidative stress and inflammatory responses in rat hepatic stellate cells |
publisher |
Taylor & Francis Group |
series |
Pharmaceutical Biology |
issn |
1388-0209 1744-5116 |
publishDate |
2020-01-01 |
description |
Context Apigenin displays antioxidant and anti-inflammatory effects. However, effects of apigenin magnesium (AM) complex on these aspects remain unknown. Objective This study investigated the effects of AM complex on oxidative stress and inflammatory responses in hydrogen peroxide (H2O2)-induced rat hepatic stellate cells (HSCs). Materials and methods The antioxidant and anti-inflammatory effects of AM complex at concentrations of 0.625, 1.25, and 2.5 mg/mL were evaluated, comparing to HSCs treated by H2O2 alone. Cell viability, reactive oxygen species (ROS), the activity of superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), nitric oxide (NO), interleukin 6 (IL-6), and nuclear factor-kappa B (NF-κB) levels were measured. Moreover, cell apoptosis, mRNA expression levels of transforming growth factor-β (TGF-β), NF-κB, and inducible nitric oxide synthase (iNOS) were assessed. Results AM complex significantly inhibited oxidative stress and inflammatory response at concentrations of 0.625, 1.25, and 2.5 mg/mL (IC50 = 1.679 mg/mL). AM complex elevated the survival rate of H2O2-treated HSCs and had no toxic effects on HSCs. AM complex also promoted SOD activity and GSH levels but suppressed ROS, MDA, and NO levels. Additionally, AM complex decreased IL-6 and NF-κB levels, gene expression of TGF-β, NF-κB, and iNOS, as well as induced apoptosis of HSCs. Discussion and conclusions Data indicated that AM complex mitigated oxidative stress and inflammatory responses on H2O2-treated HSCs, suggesting that AM complex is a possible candidate for anti-hepatic diseases. Additional efforts, both in vivo and in humans, are required to assess of AM complex as a potential therapeutic drug in liver diseases. |
topic |
mtt redox system proinflammatory cytokines real-time pcr |
url |
http://dx.doi.org/10.1080/13880209.2020.1772840 |
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