miR-375 Inhibits Proliferation of Mouse Pancreatic Progenitor Cells by Targeting YAP1
Background/Aims: The Hippo signaling pathway regulates expansion and differentiation of stem cells and tissue progenitor cells during organ development and tissue regeneration. Previous studies have shown that YAP1, a potent effector of the Hippo signaling pathway, plays a crucial role in pancreas d...
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Cell Physiol Biochem Press GmbH & Co KG
2013-12-01
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doaj-db7dcd73f89f413bbf2ce51eb04e90602020-11-25T02:46:35ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782013-12-013261808181710.1159/000356614356614miR-375 Inhibits Proliferation of Mouse Pancreatic Progenitor Cells by Targeting YAP1Zhen-Wu ZhangTong MenRui-Cheng FengYun-Chao LiDi ZhouChun-Bo TengBackground/Aims: The Hippo signaling pathway regulates expansion and differentiation of stem cells and tissue progenitor cells during organ development and tissue regeneration. Previous studies have shown that YAP1, a potent effector of the Hippo signaling pathway, plays a crucial role in pancreas development, but the function of YAP1 in pancreatic progenitor cells is less known. Methods: The spatio-temporal expression pattern of YAP1 in mouse developing pancreata was detected by in situ hybridization. The effect of silencing YAP1 on the proliferation of pancreatic progenitor cells was analyzed by CCK-8 assay and Ki67 immunostaining. The regulation of miR-375 on YAP1 expression was determined by dual luciferase reporter assay, QRT-PCR and western blot. Finally, the influence of miR-375 on proliferation of pancreatic progenitor cells was analyzed by CCK-8 assay and Ki67 immunostaining. Results: We found that YAP1 was highly expressed in embryonic and adult pancreatic progenitor cells. Knocking down YAP1 by siRNA inhibited the proliferation of pancreatic progenitor cells. The mouse YAP1 was a target gene of miR-375, and miR-375 could target the 3' UTR of YAP1 mRNA to decrease its protein and mRNA levels. Similar to silencing YAP1 by siRNA, the proliferation of pancreatic progenitor cells was inhibited significantly by miR-375. Conclusion: Our results indicate that YAP1 is necessary for the proliferation of pancreatic progenitor cells and miR-375 participates in regulating YAP1 expression during pancreatic progenitor cells differentiation.http://www.karger.com/Article/FullText/356614PancreasProliferationProgenitor cellsmiR-375YAP1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhen-Wu Zhang Tong Men Rui-Cheng Feng Yun-Chao Li Di Zhou Chun-Bo Teng |
spellingShingle |
Zhen-Wu Zhang Tong Men Rui-Cheng Feng Yun-Chao Li Di Zhou Chun-Bo Teng miR-375 Inhibits Proliferation of Mouse Pancreatic Progenitor Cells by Targeting YAP1 Cellular Physiology and Biochemistry Pancreas Proliferation Progenitor cells miR-375 YAP1 |
author_facet |
Zhen-Wu Zhang Tong Men Rui-Cheng Feng Yun-Chao Li Di Zhou Chun-Bo Teng |
author_sort |
Zhen-Wu Zhang |
title |
miR-375 Inhibits Proliferation of Mouse Pancreatic Progenitor Cells by Targeting YAP1 |
title_short |
miR-375 Inhibits Proliferation of Mouse Pancreatic Progenitor Cells by Targeting YAP1 |
title_full |
miR-375 Inhibits Proliferation of Mouse Pancreatic Progenitor Cells by Targeting YAP1 |
title_fullStr |
miR-375 Inhibits Proliferation of Mouse Pancreatic Progenitor Cells by Targeting YAP1 |
title_full_unstemmed |
miR-375 Inhibits Proliferation of Mouse Pancreatic Progenitor Cells by Targeting YAP1 |
title_sort |
mir-375 inhibits proliferation of mouse pancreatic progenitor cells by targeting yap1 |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2013-12-01 |
description |
Background/Aims: The Hippo signaling pathway regulates expansion and differentiation of stem cells and tissue progenitor cells during organ development and tissue regeneration. Previous studies have shown that YAP1, a potent effector of the Hippo signaling pathway, plays a crucial role in pancreas development, but the function of YAP1 in pancreatic progenitor cells is less known. Methods: The spatio-temporal expression pattern of YAP1 in mouse developing pancreata was detected by in situ hybridization. The effect of silencing YAP1 on the proliferation of pancreatic progenitor cells was analyzed by CCK-8 assay and Ki67 immunostaining. The regulation of miR-375 on YAP1 expression was determined by dual luciferase reporter assay, QRT-PCR and western blot. Finally, the influence of miR-375 on proliferation of pancreatic progenitor cells was analyzed by CCK-8 assay and Ki67 immunostaining. Results: We found that YAP1 was highly expressed in embryonic and adult pancreatic progenitor cells. Knocking down YAP1 by siRNA inhibited the proliferation of pancreatic progenitor cells. The mouse YAP1 was a target gene of miR-375, and miR-375 could target the 3' UTR of YAP1 mRNA to decrease its protein and mRNA levels. Similar to silencing YAP1 by siRNA, the proliferation of pancreatic progenitor cells was inhibited significantly by miR-375. Conclusion: Our results indicate that YAP1 is necessary for the proliferation of pancreatic progenitor cells and miR-375 participates in regulating YAP1 expression during pancreatic progenitor cells differentiation. |
topic |
Pancreas Proliferation Progenitor cells miR-375 YAP1 |
url |
http://www.karger.com/Article/FullText/356614 |
work_keys_str_mv |
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