Effects of Carboxylated Multiwalled Carbon Nanotubes on the Function of Macrophages
Multiwalled carbon nanotubes (MWCNTs) have tremendous potential in many areas of research and applications. Modification of MWCNTs with carboxyl group is one of the widely used strategies to increase their water dispersibility. However, the effect of carboxylation of MWCNTs on their interaction with...
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Series: | Journal of Nanomaterials |
Online Access: | http://dx.doi.org/10.1155/2015/638760 |
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doaj-db847cd008c1463baac358a6421a2b022020-11-24T22:23:38ZengHindawi LimitedJournal of Nanomaterials1687-41101687-41292015-01-01201510.1155/2015/638760638760Effects of Carboxylated Multiwalled Carbon Nanotubes on the Function of MacrophagesXia Dong0Lanxia Liu1Dunwan Zhu2Hailing Zhang3Yingxin Li4Xigang Leng5Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, ChinaTianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, ChinaTianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, ChinaTianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, ChinaTianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, ChinaTianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, ChinaMultiwalled carbon nanotubes (MWCNTs) have tremendous potential in many areas of research and applications. Modification of MWCNTs with carboxyl group is one of the widely used strategies to increase their water dispersibility. However, the effect of carboxylation of MWCNTs on their interaction with macrophages remains unclear. The current study compared the impact of pristine MWCNTs (p-MWCNTs) and carboxylic acid functionalized MWCNTs (MWCNTs-COOH) on RAW264.7 cells by looking at the cell viability, phagocytic activity, production of cytokines (IL-1β, IL-10, IL-12, and TNF-α), and intracellular reactive oxygen species (ROS). It was revealed that exposure to either p-MWCNTs or MWCNTs-COOH induced decreased viability of murine macrophage RAW 264.7 cells and moderately elevated phagocytic activity of murine peritoneal macrophages, but no statistical significance was found between the two groups. Increased production of ROS in macrophages was induced after exposure to either p-MWCNTs or MWCNTs-COOH. However, no significantly elevated production of cytokines (IL-1β, IL-10, IL-12, and TNF-α) was observed from RAW 264.7 cells after exposure to the CNTs. Those data suggested that modification with carboxyl group did not exert obvious impact on the interaction of MWCNTs with macrophages.http://dx.doi.org/10.1155/2015/638760 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xia Dong Lanxia Liu Dunwan Zhu Hailing Zhang Yingxin Li Xigang Leng |
spellingShingle |
Xia Dong Lanxia Liu Dunwan Zhu Hailing Zhang Yingxin Li Xigang Leng Effects of Carboxylated Multiwalled Carbon Nanotubes on the Function of Macrophages Journal of Nanomaterials |
author_facet |
Xia Dong Lanxia Liu Dunwan Zhu Hailing Zhang Yingxin Li Xigang Leng |
author_sort |
Xia Dong |
title |
Effects of Carboxylated Multiwalled Carbon Nanotubes on the Function of Macrophages |
title_short |
Effects of Carboxylated Multiwalled Carbon Nanotubes on the Function of Macrophages |
title_full |
Effects of Carboxylated Multiwalled Carbon Nanotubes on the Function of Macrophages |
title_fullStr |
Effects of Carboxylated Multiwalled Carbon Nanotubes on the Function of Macrophages |
title_full_unstemmed |
Effects of Carboxylated Multiwalled Carbon Nanotubes on the Function of Macrophages |
title_sort |
effects of carboxylated multiwalled carbon nanotubes on the function of macrophages |
publisher |
Hindawi Limited |
series |
Journal of Nanomaterials |
issn |
1687-4110 1687-4129 |
publishDate |
2015-01-01 |
description |
Multiwalled carbon nanotubes (MWCNTs) have tremendous potential in many areas of research and applications. Modification of MWCNTs with carboxyl group is one of the widely used strategies to increase their water dispersibility. However, the effect of carboxylation of MWCNTs on their interaction with macrophages remains unclear. The current study compared the impact of pristine MWCNTs (p-MWCNTs) and carboxylic acid functionalized MWCNTs (MWCNTs-COOH) on RAW264.7 cells by looking at the cell viability, phagocytic activity, production of cytokines (IL-1β, IL-10, IL-12, and TNF-α), and intracellular reactive oxygen species (ROS). It was revealed that exposure to either p-MWCNTs or MWCNTs-COOH induced decreased viability of murine macrophage RAW 264.7 cells and moderately elevated phagocytic activity of murine peritoneal macrophages, but no statistical significance was found between the two groups. Increased production of ROS in macrophages was induced after exposure to either p-MWCNTs or MWCNTs-COOH. However, no significantly elevated production of cytokines (IL-1β, IL-10, IL-12, and TNF-α) was observed from RAW 264.7 cells after exposure to the CNTs. Those data suggested that modification with carboxyl group did not exert obvious impact on the interaction of MWCNTs with macrophages. |
url |
http://dx.doi.org/10.1155/2015/638760 |
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