Evaluation of metabolic changes in liver and serum of streptozotocin-induced diabetic rats after Mango diet supplementation

• Main Authors: Álvaro Fernández-Ochoa, Rosario Cázares-Camacho, Isabel Borrás-Linares, J. Abraham Domínguez-Avila, Antonio Segura-Carretero, Gustavo Adolfo González-Aguilar
• Format: Article
• Language: English
• Published: Elsevier 2020-01-01
• Series: Journal of Functional Foods
• Subjects: ‘Ataulfo’ mango; Mangifera indica L; Diabetes mellitus; Metabolomics; Mass spectrometry
• Online Access: http://www.sciencedirect.com/science/article/pii/S175646461930619X

Mango consumption has shown bioactive properties against several diseases like diabetes, therefore, we evaluated how a mango-supplemented diet affects metabolic pathways in diabetic rats. Serum and liver samples were collected from 26 rats divided into 3 groups (healthy, untreated diabetic and mango-treated diabetic) after dietary intervention and analysed using an LC-MS untargeted metabolomic strategy. Twenty-six and 29 metabolites in serum and liver were potentially annotated, showing significant differences among groups. Several affected pathways were due to the disease state [fatty acids (i.e. palmitoleic, linolenic, stearidonic, eicosapentaenoic, docosahexaenoic acids and others), bile acids (i.e. glycocholic acid) and amino acids (i.e. leucine, isoleucine and valine)], and others due to mango supplementation (increased hepatic bioaccumulation of euxanthone, a mangiferin metabolite, and increased glutathione concentration). These results suggest that a mango-supplemented diet exerted a significant antioxidant effect in the liver of diabetic rats, likely due to its phenolic compounds, like mangiferin and its metabolites.