Mexican Trypanosoma cruzi stocks: analysis of minicircle kDNA homologies by cross-hybridization

• Main Authors: Marie-France Bosseno, Bertha Espinoza, Beatriz Sánchez, Simone Frédérique Brenière
• Format: Article
• Language: English
• Published: Instituto Oswaldo Cruz, Ministério da Saúde 2000-08-01
• Series: Memórias do Instituto Oswaldo Cruz.
• Subjects: Trypanosoma cruzi; Mexico; kDNA minicircle
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000400005

Homologies of minicircle kDNA of 27 Mexican stocks were studied by cross-hybridization with four kDNA probes derived from three reference stocks belonging to groups Trypanosoma cruzi I (SO34 cl4 and Silvio) and T. cruzi II (MN) and one Mexican stock. High homologies were only observed with Silvio (six stocks) and Mexican probes (11 stocks). After 30 min exposure (low homology) additional stocks were recognized with SO34 cl4 (three stocks) and Silvio (six stocks) probes; with the Mexican probe only five stocks remained non-reactive. All the stocks were typed by isoenzyme (16 loci) and Mexican stocks belonged to T. cruzi I. Hybridization patterns were not strictly correlated with the observed clustering and cross-hybridization of kDNA minicircles is not available to distinct Mexican stocks.