Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes
A growing number of tet(X)-type tigecycline resistance determinants [tet(X1) to tet(X5)] constitutes an expanding family of tetracycline-inactivating enzymes, posing a potential risk to global public health. Here, we report the development of an efficient multiplex PCR method to detect the family of...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2020-01-01
|
Series: | Virulence |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/21505594.2019.1706913 |
id |
doaj-db9e4eb765a54fa6bb02b807dff7a747 |
---|---|
record_format |
Article |
spelling |
doaj-db9e4eb765a54fa6bb02b807dff7a7472021-01-15T13:32:57ZengTaylor & Francis GroupVirulence2150-55942150-56082020-01-01111495610.1080/21505594.2019.17069131706913Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genesKai Ji0Yongchang Xu1Jian Sun2Man Huang3Xu Jia4Chengjian Jiang5Youjun Feng6College of Life Science and Technology, Guangxi UniversityZhejiang University School of MedicineSouth China Agricultural UniversityZhejiang University School of MedicineNon-coding RNA and Drug Discovery Key Laboratory of Sichuan Province, Chengdu Medical CollegeCollege of Life Science and Technology, Guangxi UniversityCollege of Life Science and Technology, Guangxi UniversityA growing number of tet(X)-type tigecycline resistance determinants [tet(X1) to tet(X5)] constitutes an expanding family of tetracycline-inactivating enzymes, posing a potential risk to global public health. Here, we report the development of an efficient multiplex PCR method to detect the family of tet(X) variants. This method is successfully applied in the screen and validation of tet(X) genes in the field and clinic bacterial samples. In addition, we found that the formerly proposed tet(X1) is a premature truncated version by the inappropriate annotation, and fixed this error. Overall, it might be the first genetic tool for the detection of different Tet(X) members.http://dx.doi.org/10.1080/21505594.2019.1706913multiplex pcrantimicrobial resistancetigecycline resistancetet(x)tet(x1)tet(x2)tet(x3)tet(x4)tet(x5) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kai Ji Yongchang Xu Jian Sun Man Huang Xu Jia Chengjian Jiang Youjun Feng |
spellingShingle |
Kai Ji Yongchang Xu Jian Sun Man Huang Xu Jia Chengjian Jiang Youjun Feng Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes Virulence multiplex pcr antimicrobial resistance tigecycline resistance tet(x) tet(x1) tet(x2) tet(x3) tet(x4) tet(x5) |
author_facet |
Kai Ji Yongchang Xu Jian Sun Man Huang Xu Jia Chengjian Jiang Youjun Feng |
author_sort |
Kai Ji |
title |
Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes |
title_short |
Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes |
title_full |
Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes |
title_fullStr |
Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes |
title_full_unstemmed |
Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes |
title_sort |
harnessing efficient multiplex pcr methods to detect the expanding tet(x) family of tigecycline resistance genes |
publisher |
Taylor & Francis Group |
series |
Virulence |
issn |
2150-5594 2150-5608 |
publishDate |
2020-01-01 |
description |
A growing number of tet(X)-type tigecycline resistance determinants [tet(X1) to tet(X5)] constitutes an expanding family of tetracycline-inactivating enzymes, posing a potential risk to global public health. Here, we report the development of an efficient multiplex PCR method to detect the family of tet(X) variants. This method is successfully applied in the screen and validation of tet(X) genes in the field and clinic bacterial samples. In addition, we found that the formerly proposed tet(X1) is a premature truncated version by the inappropriate annotation, and fixed this error. Overall, it might be the first genetic tool for the detection of different Tet(X) members. |
topic |
multiplex pcr antimicrobial resistance tigecycline resistance tet(x) tet(x1) tet(x2) tet(x3) tet(x4) tet(x5) |
url |
http://dx.doi.org/10.1080/21505594.2019.1706913 |
work_keys_str_mv |
AT kaiji harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes AT yongchangxu harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes AT jiansun harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes AT manhuang harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes AT xujia harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes AT chengjianjiang harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes AT youjunfeng harnessingefficientmultiplexpcrmethodstodetecttheexpandingtetxfamilyoftigecyclineresistancegenes |
_version_ |
1714944425448177664 |