A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamiana
Abstract Background Nicotiana benthamiana is an important model organism of the Solanaceae (Nightshade) family. Several draft assemblies of the N. benthamiana genome have been generated, but many of the gene-models in these draft assemblies appear incorrect. Results Here we present an improved prote...
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doaj-dba28177f35541938d9f7b80d2e42e532020-11-25T03:23:50ZengBMCBMC Genomics1471-21642019-10-0120111510.1186/s12864-019-6058-6A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamianaJiorgos Kourelis0Farnusch Kaschani1Friederike M. Grosse-Holz2Felix Homma3Markus Kaiser4Renier A. L. van der Hoorn5Plant Chemetics Laboratory, Department of Plant Sciences, University of OxfordChemische Biologie, Zentrum fur Medizinische Biotechnologie, Fakultät für Biologie, Universität Duisburg-EssenPlant Chemetics Laboratory, Department of Plant Sciences, University of OxfordPlant Chemetics Laboratory, Department of Plant Sciences, University of OxfordChemische Biologie, Zentrum fur Medizinische Biotechnologie, Fakultät für Biologie, Universität Duisburg-EssenPlant Chemetics Laboratory, Department of Plant Sciences, University of OxfordAbstract Background Nicotiana benthamiana is an important model organism of the Solanaceae (Nightshade) family. Several draft assemblies of the N. benthamiana genome have been generated, but many of the gene-models in these draft assemblies appear incorrect. Results Here we present an improved proteome based on the Niben1.0.1 draft genome assembly guided by gene models from other Nicotiana species. Due to the fragmented nature of the Niben1.0.1 draft genome, many protein-encoding genes are missing or partial. We complement these missing proteins by similarly annotating other draft genome assemblies. This approach overcomes problems caused by mis-annotated exon-intron boundaries and mis-assigned short read transcripts to homeologs in polyploid genomes. With an estimated 98.1% completeness; only 53,411 protein-encoding genes; and improved protein lengths and functional annotations, this new predicted proteome is better in assigning spectra than the preceding proteome annotations. This dataset is more sensitive and accurate in proteomics applications, clarifying the detection by activity-based proteomics of proteins that were previously predicted to be inactive. Phylogenetic analysis of the subtilase family of hydrolases reveal inactivation of likely homeologs, associated with a contraction of the functional genome in this alloploid plant species. Finally, we use this new proteome annotation to characterize the extracellular proteome as compared to a total leaf proteome, which highlights the enrichment of hydrolases in the apoplast. Conclusions This proteome annotation provides the community working with Nicotiana benthamiana with an important new resource for functional proteomics.http://link.springer.com/article/10.1186/s12864-019-6058-6SolanaceaeGenome annotationNicotiana benthamianaProteomicsSubtilases |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jiorgos Kourelis Farnusch Kaschani Friederike M. Grosse-Holz Felix Homma Markus Kaiser Renier A. L. van der Hoorn |
spellingShingle |
Jiorgos Kourelis Farnusch Kaschani Friederike M. Grosse-Holz Felix Homma Markus Kaiser Renier A. L. van der Hoorn A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamiana BMC Genomics Solanaceae Genome annotation Nicotiana benthamiana Proteomics Subtilases |
author_facet |
Jiorgos Kourelis Farnusch Kaschani Friederike M. Grosse-Holz Felix Homma Markus Kaiser Renier A. L. van der Hoorn |
author_sort |
Jiorgos Kourelis |
title |
A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamiana |
title_short |
A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamiana |
title_full |
A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamiana |
title_fullStr |
A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamiana |
title_full_unstemmed |
A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamiana |
title_sort |
homology-guided, genome-based proteome for improved proteomics in the alloploid nicotiana benthamiana |
publisher |
BMC |
series |
BMC Genomics |
issn |
1471-2164 |
publishDate |
2019-10-01 |
description |
Abstract Background Nicotiana benthamiana is an important model organism of the Solanaceae (Nightshade) family. Several draft assemblies of the N. benthamiana genome have been generated, but many of the gene-models in these draft assemblies appear incorrect. Results Here we present an improved proteome based on the Niben1.0.1 draft genome assembly guided by gene models from other Nicotiana species. Due to the fragmented nature of the Niben1.0.1 draft genome, many protein-encoding genes are missing or partial. We complement these missing proteins by similarly annotating other draft genome assemblies. This approach overcomes problems caused by mis-annotated exon-intron boundaries and mis-assigned short read transcripts to homeologs in polyploid genomes. With an estimated 98.1% completeness; only 53,411 protein-encoding genes; and improved protein lengths and functional annotations, this new predicted proteome is better in assigning spectra than the preceding proteome annotations. This dataset is more sensitive and accurate in proteomics applications, clarifying the detection by activity-based proteomics of proteins that were previously predicted to be inactive. Phylogenetic analysis of the subtilase family of hydrolases reveal inactivation of likely homeologs, associated with a contraction of the functional genome in this alloploid plant species. Finally, we use this new proteome annotation to characterize the extracellular proteome as compared to a total leaf proteome, which highlights the enrichment of hydrolases in the apoplast. Conclusions This proteome annotation provides the community working with Nicotiana benthamiana with an important new resource for functional proteomics. |
topic |
Solanaceae Genome annotation Nicotiana benthamiana Proteomics Subtilases |
url |
http://link.springer.com/article/10.1186/s12864-019-6058-6 |
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