Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates

Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates

A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name “Therminator DNA polymerase” has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabl...

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Bibliographic Details
Main Authors: Andrew F. Gardner, Kiserian M. Jackson, Madeleine M. Boyle, Jackson A. Buss, Vladimir Potapov, Alexandra M. Gehring, Kelly M. Zatopek, Ivan R. Corrêa Jr., Jennifer L. Ong, William E. Jack
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Molecular Biosciences
Subjects:
DNA sequencing
xenonucleic acids
aptamers
functionalized DNA
unnatural base pairs
Online Access:https://www.frontiersin.org/article/10.3389/fmolb.2019.00028/full
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spelling doaj-dbab20a8f931428eabdea0cacf437dd82020-11-24T21:09:31ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2019-04-01610.3389/fmolb.2019.00028441577Therminator DNA Polymerase: Modified Nucleotides and Unnatural SubstratesAndrew F. GardnerKiserian M. JacksonMadeleine M. BoyleJackson A. BussVladimir PotapovAlexandra M. GehringKelly M. ZatopekIvan R. Corrêa Jr.Jennifer L. OngWilliam E. JackA variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name “Therminator DNA polymerase” has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabled new technologies in synthetic biology and DNA sequencing. In addition, we discuss mechanisms for increased modified nucleotide incorporation.https://www.frontiersin.org/article/10.3389/fmolb.2019.00028/fullDNA sequencingxenonucleic acidsaptamersfunctionalized DNAunnatural base pairs
collection DOAJ
language English
format Article
sources DOAJ
author Andrew F. Gardner
Kiserian M. Jackson
Madeleine M. Boyle
Jackson A. Buss
Vladimir Potapov
Alexandra M. Gehring
Kelly M. Zatopek
Ivan R. Corrêa Jr.
Jennifer L. Ong
William E. Jack
spellingShingle Andrew F. Gardner
Kiserian M. Jackson
Madeleine M. Boyle
Jackson A. Buss
Vladimir Potapov
Alexandra M. Gehring
Kelly M. Zatopek
Ivan R. Corrêa Jr.
Jennifer L. Ong
William E. Jack
Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
Frontiers in Molecular Biosciences
DNA sequencing
xenonucleic acids
aptamers
functionalized DNA
unnatural base pairs
author_facet Andrew F. Gardner
Kiserian M. Jackson
Madeleine M. Boyle
Jackson A. Buss
Vladimir Potapov
Alexandra M. Gehring
Kelly M. Zatopek
Ivan R. Corrêa Jr.
Jennifer L. Ong
William E. Jack
author_sort Andrew F. Gardner
title Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
title_short Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
title_full Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
title_fullStr Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
title_full_unstemmed Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
title_sort therminator dna polymerase: modified nucleotides and unnatural substrates
publisher Frontiers Media S.A.
series Frontiers in Molecular Biosciences
issn 2296-889X
publishDate 2019-04-01
description A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name “Therminator DNA polymerase” has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabled new technologies in synthetic biology and DNA sequencing. In addition, we discuss mechanisms for increased modified nucleotide incorporation.
topic DNA sequencing
xenonucleic acids
aptamers
functionalized DNA
unnatural base pairs
url https://www.frontiersin.org/article/10.3389/fmolb.2019.00028/full
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