Development of Polythiourethane/ZnO-Based Anti-Fouling Materials and Evaluation of the Adhesion of <i>Staphylococcus aureus</i> and <i>Candida glabrata</i> Using Single-Cell Force Spectroscopy
The attachment of bacteria and other microbes to natural and artificial surfaces leads to the development of biofilms, which can further cause nosocomial infections. Thus, an important field of research is the development of new materials capable of preventing the initial adhesion of pathogenic micr...
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doaj-dbaed8c3b8e746079fae9c8320e8af392021-01-22T00:00:35ZengMDPI AGNanomaterials2079-49912021-01-011127127110.3390/nano11020271Development of Polythiourethane/ZnO-Based Anti-Fouling Materials and Evaluation of the Adhesion of <i>Staphylococcus aureus</i> and <i>Candida glabrata</i> Using Single-Cell Force SpectroscopySophie Klemm0Martina Baum1Haoyi Qiu2Zibin Nan3Mafalda Cavalheiro4Miguel Cacho Teixeira5Claire Tendero6Anna Gapeeva7Rainer Adelung8Etienne Dague9Mickaël Castelain10Cécile Formosa-Dague11Functional Nanomaterials, Institute for Materials Science, Kiel University, 24143 Kiel, GermanyFunctional Nanomaterials, Institute for Materials Science, Kiel University, 24143 Kiel, GermanyFunctional Nanomaterials, Institute for Materials Science, Kiel University, 24143 Kiel, GermanyTBI, Université de Toulouse, INSA, INRAE, CNRS, 31400 Toulouse, FranceInstitute for Bioengineering and Biosciences (iBB), Instituto Superior Técnico, University of Lisbon, 1049-001 Lisbon, PortugalInstitute for Bioengineering and Biosciences (iBB), Instituto Superior Técnico, University of Lisbon, 1049-001 Lisbon, PortugalCIRIMAT, Université de Toulouse, CNRS, INPT, UPS, 31400 Toulouse, FranceFunctional Nanomaterials, Institute for Materials Science, Kiel University, 24143 Kiel, GermanyFunctional Nanomaterials, Institute for Materials Science, Kiel University, 24143 Kiel, GermanyLAAS-CNRS, Université de Toulouse, CNRS, 31400 Toulouse, FranceTBI, Université de Toulouse, INSA, INRAE, CNRS, 31400 Toulouse, FranceTBI, Université de Toulouse, INSA, INRAE, CNRS, 31400 Toulouse, FranceThe attachment of bacteria and other microbes to natural and artificial surfaces leads to the development of biofilms, which can further cause nosocomial infections. Thus, an important field of research is the development of new materials capable of preventing the initial adhesion of pathogenic microorganisms. In this work, novel polymer/particle composite materials, based on a polythiourethane (PTU) matrix and either spherical (s-ZnO) or tetrapodal (t-ZnO) shaped ZnO fillers, were developed and characterized with respect to their mechanical, chemical and surface properties. To then evaluate their potential as anti-fouling surfaces, the adhesion of two different pathogenic microorganism species, <i>Staphylococcus aureus</i> and <i>Candida glabrata,</i> was studied using atomic force microscopy (AFM). Our results show that the adhesion of both <i>S. aureus</i> and <i>C. glabrata</i> to PTU and PTU/ZnO is decreased compared to a model surface polydimethylsiloxane (PDMS). It was furthermore found that the amount of both s-ZnO and t-ZnO filler had a direct influence on the adhesion of <i>S. aureus</i>, as increasing amounts of ZnO particles resulted in reduced adhesion of the cells. For both microorganisms, material composites with 5 wt.% of t-ZnO particles showed the greatest potential for anti-fouling with significantly decreased adhesion of cells. Altogether, both pathogens exhibit a reduced capacity to adhere to the newly developed nanomaterials used in this study, thus showing their potential for bio-medical applications.https://www.mdpi.com/2079-4991/11/2/271polythiourethanetetrapodal shaped ZnOPTU/ZnO compositemicrobial adhesioninitial attachment<i>S. aureus</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sophie Klemm Martina Baum Haoyi Qiu Zibin Nan Mafalda Cavalheiro Miguel Cacho Teixeira Claire Tendero Anna Gapeeva Rainer Adelung Etienne Dague Mickaël Castelain Cécile Formosa-Dague |
spellingShingle |
Sophie Klemm Martina Baum Haoyi Qiu Zibin Nan Mafalda Cavalheiro Miguel Cacho Teixeira Claire Tendero Anna Gapeeva Rainer Adelung Etienne Dague Mickaël Castelain Cécile Formosa-Dague Development of Polythiourethane/ZnO-Based Anti-Fouling Materials and Evaluation of the Adhesion of <i>Staphylococcus aureus</i> and <i>Candida glabrata</i> Using Single-Cell Force Spectroscopy Nanomaterials polythiourethane tetrapodal shaped ZnO PTU/ZnO composite microbial adhesion initial attachment <i>S. aureus</i> |
author_facet |
Sophie Klemm Martina Baum Haoyi Qiu Zibin Nan Mafalda Cavalheiro Miguel Cacho Teixeira Claire Tendero Anna Gapeeva Rainer Adelung Etienne Dague Mickaël Castelain Cécile Formosa-Dague |
author_sort |
Sophie Klemm |
title |
Development of Polythiourethane/ZnO-Based Anti-Fouling Materials and Evaluation of the Adhesion of <i>Staphylococcus aureus</i> and <i>Candida glabrata</i> Using Single-Cell Force Spectroscopy |
title_short |
Development of Polythiourethane/ZnO-Based Anti-Fouling Materials and Evaluation of the Adhesion of <i>Staphylococcus aureus</i> and <i>Candida glabrata</i> Using Single-Cell Force Spectroscopy |
title_full |
Development of Polythiourethane/ZnO-Based Anti-Fouling Materials and Evaluation of the Adhesion of <i>Staphylococcus aureus</i> and <i>Candida glabrata</i> Using Single-Cell Force Spectroscopy |
title_fullStr |
Development of Polythiourethane/ZnO-Based Anti-Fouling Materials and Evaluation of the Adhesion of <i>Staphylococcus aureus</i> and <i>Candida glabrata</i> Using Single-Cell Force Spectroscopy |
title_full_unstemmed |
Development of Polythiourethane/ZnO-Based Anti-Fouling Materials and Evaluation of the Adhesion of <i>Staphylococcus aureus</i> and <i>Candida glabrata</i> Using Single-Cell Force Spectroscopy |
title_sort |
development of polythiourethane/zno-based anti-fouling materials and evaluation of the adhesion of <i>staphylococcus aureus</i> and <i>candida glabrata</i> using single-cell force spectroscopy |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2021-01-01 |
description |
The attachment of bacteria and other microbes to natural and artificial surfaces leads to the development of biofilms, which can further cause nosocomial infections. Thus, an important field of research is the development of new materials capable of preventing the initial adhesion of pathogenic microorganisms. In this work, novel polymer/particle composite materials, based on a polythiourethane (PTU) matrix and either spherical (s-ZnO) or tetrapodal (t-ZnO) shaped ZnO fillers, were developed and characterized with respect to their mechanical, chemical and surface properties. To then evaluate their potential as anti-fouling surfaces, the adhesion of two different pathogenic microorganism species, <i>Staphylococcus aureus</i> and <i>Candida glabrata,</i> was studied using atomic force microscopy (AFM). Our results show that the adhesion of both <i>S. aureus</i> and <i>C. glabrata</i> to PTU and PTU/ZnO is decreased compared to a model surface polydimethylsiloxane (PDMS). It was furthermore found that the amount of both s-ZnO and t-ZnO filler had a direct influence on the adhesion of <i>S. aureus</i>, as increasing amounts of ZnO particles resulted in reduced adhesion of the cells. For both microorganisms, material composites with 5 wt.% of t-ZnO particles showed the greatest potential for anti-fouling with significantly decreased adhesion of cells. Altogether, both pathogens exhibit a reduced capacity to adhere to the newly developed nanomaterials used in this study, thus showing their potential for bio-medical applications. |
topic |
polythiourethane tetrapodal shaped ZnO PTU/ZnO composite microbial adhesion initial attachment <i>S. aureus</i> |
url |
https://www.mdpi.com/2079-4991/11/2/271 |
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