Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus

Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus

Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus–based neutralization assay with preliminary assessment...

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Bibliographic Details
Main Authors: Chao Qiu, Yang Huang, Anli Zhang, Di Tian, Yanmin Wan, Xiaoling Zhang, Wanju Zhang, Zhiyong Zhang, Zhenghong Yuan, Yunwen Hu, Xiaoyan Zhang, Jianqing Xu
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2013-10-01
Series:Emerging Infectious Diseases
Subjects:
Influenza
subtype H7N9
serology
neutralization tests
pseudovirus
viruses
Online Access:https://wwwnc.cdc.gov/eid/article/19/10/13-0728_article
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spelling doaj-dbb27cdc8905430388c8ac640c6930412020-11-24T23:34:58ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592013-10-0119101685168710.3201/eid1910.130728Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) VirusChao QiuYang HuangAnli ZhangDi TianYanmin WanXiaoling ZhangWanju ZhangZhiyong ZhangZhenghong YuanYunwen HuXiaoyan ZhangJianqing XuSerologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus–based neutralization assay with preliminary assessment using subtype H7N9–infected samples and controls.https://wwwnc.cdc.gov/eid/article/19/10/13-0728_articleInfluenzasubtype H7N9serologyneutralization testspseudovirusviruses
collection DOAJ
language English
format Article
sources DOAJ
author Chao Qiu
Yang Huang
Anli Zhang
Di Tian
Yanmin Wan
Xiaoling Zhang
Wanju Zhang
Zhiyong Zhang
Zhenghong Yuan
Yunwen Hu
Xiaoyan Zhang
Jianqing Xu
spellingShingle Chao Qiu
Yang Huang
Anli Zhang
Di Tian
Yanmin Wan
Xiaoling Zhang
Wanju Zhang
Zhiyong Zhang
Zhenghong Yuan
Yunwen Hu
Xiaoyan Zhang
Jianqing Xu
Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
Emerging Infectious Diseases
Influenza
subtype H7N9
serology
neutralization tests
pseudovirus
viruses
author_facet Chao Qiu
Yang Huang
Anli Zhang
Di Tian
Yanmin Wan
Xiaoling Zhang
Wanju Zhang
Zhiyong Zhang
Zhenghong Yuan
Yunwen Hu
Xiaoyan Zhang
Jianqing Xu
author_sort Chao Qiu
title Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_short Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_full Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_fullStr Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_full_unstemmed Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_sort safe pseudovirus-based assay for neutralization antibodies against influenza a(h7n9) virus
publisher Centers for Disease Control and Prevention
series Emerging Infectious Diseases
issn 1080-6040
1080-6059
publishDate 2013-10-01
description Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus–based neutralization assay with preliminary assessment using subtype H7N9–infected samples and controls.
topic Influenza
subtype H7N9
serology
neutralization tests
pseudovirus
viruses
url https://wwwnc.cdc.gov/eid/article/19/10/13-0728_article
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