The Interactions of Airway Bacterial and Fungal Communities in Clinically Stable Asthma
Dysbiotic airway microbiota play important roles in the inflammatory progression of asthma, and exploration of airway microbial interactions further elucidates asthma pathogenesis. However, little is known regarding the airway bacterial-fungal interactions in asthma patients. We conducted a cross-se...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-07-01
|
Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01647/full |
id |
doaj-dbc430ababc64f01a48839cae427aae1 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hai-yue Liu Hai-yue Liu Chun-xi Li Zhen-yu Liang Shi-yu Zhang Wan-ying Yang Yan-mei Ye Yan-xia Lin Rong-chang Chen Hong-wei Zhou Jin Su |
spellingShingle |
Hai-yue Liu Hai-yue Liu Chun-xi Li Zhen-yu Liang Shi-yu Zhang Wan-ying Yang Yan-mei Ye Yan-xia Lin Rong-chang Chen Hong-wei Zhou Jin Su The Interactions of Airway Bacterial and Fungal Communities in Clinically Stable Asthma Frontiers in Microbiology 16S rRNA airway microbiome asthma bacterial-fungal interactions ITS |
author_facet |
Hai-yue Liu Hai-yue Liu Chun-xi Li Zhen-yu Liang Shi-yu Zhang Wan-ying Yang Yan-mei Ye Yan-xia Lin Rong-chang Chen Hong-wei Zhou Jin Su |
author_sort |
Hai-yue Liu |
title |
The Interactions of Airway Bacterial and Fungal Communities in Clinically Stable Asthma |
title_short |
The Interactions of Airway Bacterial and Fungal Communities in Clinically Stable Asthma |
title_full |
The Interactions of Airway Bacterial and Fungal Communities in Clinically Stable Asthma |
title_fullStr |
The Interactions of Airway Bacterial and Fungal Communities in Clinically Stable Asthma |
title_full_unstemmed |
The Interactions of Airway Bacterial and Fungal Communities in Clinically Stable Asthma |
title_sort |
interactions of airway bacterial and fungal communities in clinically stable asthma |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-07-01 |
description |
Dysbiotic airway microbiota play important roles in the inflammatory progression of asthma, and exploration of airway microbial interactions further elucidates asthma pathogenesis. However, little is known regarding the airway bacterial-fungal interactions in asthma patients. We conducted a cross-sectional survey of the sputum bacterial and fungal microbiota from 116 clinically stable asthma patients and 29 healthy controls using 16S rRNA gene and ITS1 sequencing. Compared with healthy individuals, asthma patients exhibited a significantly altered microbiota and increased bacterial and fungal alpha diversities in the airway. Microbial genera Moraxella, Capnocytophaga, and Ralstonia (bacteria) and Schizophyllum, Candida, and Phialemoniopsis (fungi) were more abundant in the asthma airways, while Rothia, Veillonella and Leptotrichia (bacteria) and Meyerozyma (fungus) were increased in healthy controls. The Moraxellaceae family and their genus Moraxella were significantly enriched in asthma patients compared with healthy controls (80.5-fold, P = 0.007 and 314.7-fold, P = 0.027, respectively). Moreover, Moraxellaceae, along with Schizophyllum, Candida, and Aspergillus (fungal genera), were positively associated with fungal alpha diversity. Correlation networks revealed 3 fungal genera (Schizophyllum, Candida, and Aspergillus) as important airway microbes in asthma that showed positive correlations with each other and multiple co-exclusions with other common microbiota. Moraxellaceae members were positively associated with asthma-enriched fungal taxa but negatively related to several healthy-enriched bacterial taxa. Collectively, our findings revealed an altered microbiota and complex microbial interactions in the airways of asthma patients. The Moraxellaceae family and their genus Moraxella, along with 3 important fungal taxa, showed significant interactions with the airway microbiota, providing potential insights into the novel pathogenic mechanisms of asthma. |
topic |
16S rRNA airway microbiome asthma bacterial-fungal interactions ITS |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2020.01647/full |
work_keys_str_mv |
AT haiyueliu theinteractionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT haiyueliu theinteractionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT chunxili theinteractionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT zhenyuliang theinteractionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT shiyuzhang theinteractionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT wanyingyang theinteractionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT yanmeiye theinteractionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT yanxialin theinteractionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT rongchangchen theinteractionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT hongweizhou theinteractionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT jinsu theinteractionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT haiyueliu interactionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT haiyueliu interactionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT chunxili interactionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT zhenyuliang interactionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT shiyuzhang interactionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT wanyingyang interactionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT yanmeiye interactionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT yanxialin interactionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT rongchangchen interactionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT hongweizhou interactionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma AT jinsu interactionsofairwaybacterialandfungalcommunitiesinclinicallystableasthma |
_version_ |
1724670216247443456 |
spelling |
doaj-dbc430ababc64f01a48839cae427aae12020-11-25T03:07:29ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-07-011110.3389/fmicb.2020.01647533219The Interactions of Airway Bacterial and Fungal Communities in Clinically Stable AsthmaHai-yue Liu0Hai-yue Liu1Chun-xi Li2Zhen-yu Liang3Shi-yu Zhang4Wan-ying Yang5Yan-mei Ye6Yan-xia Lin7Rong-chang Chen8Hong-wei Zhou9Jin Su10Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaState Key Laboratory of Organ Failure Research, Microbiome Medicine Center, Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaChronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaChronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaChronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaChronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaChronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaState Key Laboratory of Organ Failure Research, Microbiome Medicine Center, Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaChronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDysbiotic airway microbiota play important roles in the inflammatory progression of asthma, and exploration of airway microbial interactions further elucidates asthma pathogenesis. However, little is known regarding the airway bacterial-fungal interactions in asthma patients. We conducted a cross-sectional survey of the sputum bacterial and fungal microbiota from 116 clinically stable asthma patients and 29 healthy controls using 16S rRNA gene and ITS1 sequencing. Compared with healthy individuals, asthma patients exhibited a significantly altered microbiota and increased bacterial and fungal alpha diversities in the airway. Microbial genera Moraxella, Capnocytophaga, and Ralstonia (bacteria) and Schizophyllum, Candida, and Phialemoniopsis (fungi) were more abundant in the asthma airways, while Rothia, Veillonella and Leptotrichia (bacteria) and Meyerozyma (fungus) were increased in healthy controls. The Moraxellaceae family and their genus Moraxella were significantly enriched in asthma patients compared with healthy controls (80.5-fold, P = 0.007 and 314.7-fold, P = 0.027, respectively). Moreover, Moraxellaceae, along with Schizophyllum, Candida, and Aspergillus (fungal genera), were positively associated with fungal alpha diversity. Correlation networks revealed 3 fungal genera (Schizophyllum, Candida, and Aspergillus) as important airway microbes in asthma that showed positive correlations with each other and multiple co-exclusions with other common microbiota. Moraxellaceae members were positively associated with asthma-enriched fungal taxa but negatively related to several healthy-enriched bacterial taxa. Collectively, our findings revealed an altered microbiota and complex microbial interactions in the airways of asthma patients. The Moraxellaceae family and their genus Moraxella, along with 3 important fungal taxa, showed significant interactions with the airway microbiota, providing potential insights into the novel pathogenic mechanisms of asthma.https://www.frontiersin.org/article/10.3389/fmicb.2020.01647/full16S rRNAairway microbiomeasthmabacterial-fungal interactionsITS |