Development and Preliminary Assessment of Hemoperfusion Cartridge with Tannic Acid for Toxic Proteins' Precipitation: An In Vitro Model
Charcoal hemoperfusion (CHP) is one of the extracorporeal removal techniques that are used to remove toxins from the body. CHP generally is considered the preferred method for extracorporeal extraction of several toxins—toxins that are adsorbed by activated charcoal. Assessments of the tannic acid...
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doaj-dbceef1f386748ea9875cb0f637767882020-11-25T00:19:03ZengHATASORecent Advances in Biology and Medicine2378-654X2378-654X2016-09-0122016627010.18639/RABM.2016.02.292685292685Development and Preliminary Assessment of Hemoperfusion Cartridge with Tannic Acid for Toxic Proteins' Precipitation: An In Vitro ModelValquíria Miwa Hanai Yoshida0Roberta Lima Cavalcante1Jessica Campanholi2Élvio Franco de Camargo Aranha3Maximilian Estevan Oliveira4Edson Hideaki Yoshida5Yoko Oshima-Franco6Biomaterials and Nanotechnology Laboratory, University of Sorocaba, Sorocaba, SP, Brazil.Veterinary Medicine Course, University of Sorocaba, Sorocaba, SP, Brazil.Veterinary Medicine Course, University of Sorocaba, Sorocaba, SP, Brazil.Institute Dante Pazzanese of Cardiology, Faculty of Medicine, University of São Paulo, São Paulo, SP, Brazil.Post-Graduate Program in Pharmaceutical Sciences, University of Sorocaba, Sorocaba, SP, Brazil.Post-Graduate Program in Pharmaceutical Sciences, University of Sorocaba, Sorocaba, SP, Brazil.Veterinary Medicine Course, University of Sorocaba, Sorocaba, SP, Brazil.Charcoal hemoperfusion (CHP) is one of the extracorporeal removal techniques that are used to remove toxins from the body. CHP generally is considered the preferred method for extracorporeal extraction of several toxins—toxins that are adsorbed by activated charcoal. Assessments of the tannic acid's protective effects on ophidian poisoning are associated with the toxic proteins' precipitation by tannic acid. The challenge in treating a snakebite lies in removing the injected poison with minimal damage to blood constituent proteins. An alternative is CHP, and this investigation proposed to develop a column for hemoperfuser cartridge, combining charcoal granules trapped between layers of polymeric material conjugated to tannic acid, using an in vitro model scaled to the Wistar rat, which can be tested in an animal model. The cartridge was evaluated using the 22 full factorial design, in duplicate, as a method to study the effects of granulated-charcoal size and tannic acid concentration on the hematologic profile (platelet and leukocyte counts) and biochemical profile (total serum protein and albumin dosages) of sheep blood. The results demonstrate that charcoal in hemoperfuser cartridge: (1) decreases the serum in sheep blood volume, as consequence, (2) increases the serum proteins' concentration, and (iii) exerts slight influence on albumin. The inclusion of tannic acid in hemoperfuser column precipitates some of serum proteins and albumin, decreasing their concentrations in the plasma serum. In conclusion, based on these effects we can suggest the use of 0.02 g tannic acid concentration and 8–20 mesh granulated charcoal in hemoperfuser cartridge for precipitating toxic proteins from snake venoms.https://rabm.scholasticahq.com/article/900-development-and-preliminary-assessment-of-hemoperfusion-cartridge-with-tannic-acid-for-toxic-proteins-precipitation-an-in-vitro-modelCharcoal granulesHemoperfusionSnake venomTannic acid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Valquíria Miwa Hanai Yoshida Roberta Lima Cavalcante Jessica Campanholi Élvio Franco de Camargo Aranha Maximilian Estevan Oliveira Edson Hideaki Yoshida Yoko Oshima-Franco |
spellingShingle |
Valquíria Miwa Hanai Yoshida Roberta Lima Cavalcante Jessica Campanholi Élvio Franco de Camargo Aranha Maximilian Estevan Oliveira Edson Hideaki Yoshida Yoko Oshima-Franco Development and Preliminary Assessment of Hemoperfusion Cartridge with Tannic Acid for Toxic Proteins' Precipitation: An In Vitro Model Recent Advances in Biology and Medicine Charcoal granules Hemoperfusion Snake venom Tannic acid |
author_facet |
Valquíria Miwa Hanai Yoshida Roberta Lima Cavalcante Jessica Campanholi Élvio Franco de Camargo Aranha Maximilian Estevan Oliveira Edson Hideaki Yoshida Yoko Oshima-Franco |
author_sort |
Valquíria Miwa Hanai Yoshida |
title |
Development and Preliminary Assessment of Hemoperfusion Cartridge with Tannic Acid for Toxic Proteins' Precipitation: An In Vitro Model |
title_short |
Development and Preliminary Assessment of Hemoperfusion Cartridge with Tannic Acid for Toxic Proteins' Precipitation: An In Vitro Model |
title_full |
Development and Preliminary Assessment of Hemoperfusion Cartridge with Tannic Acid for Toxic Proteins' Precipitation: An In Vitro Model |
title_fullStr |
Development and Preliminary Assessment of Hemoperfusion Cartridge with Tannic Acid for Toxic Proteins' Precipitation: An In Vitro Model |
title_full_unstemmed |
Development and Preliminary Assessment of Hemoperfusion Cartridge with Tannic Acid for Toxic Proteins' Precipitation: An In Vitro Model |
title_sort |
development and preliminary assessment of hemoperfusion cartridge with tannic acid for toxic proteins' precipitation: an in vitro model |
publisher |
HATASO |
series |
Recent Advances in Biology and Medicine |
issn |
2378-654X 2378-654X |
publishDate |
2016-09-01 |
description |
Charcoal hemoperfusion (CHP) is one of the extracorporeal removal techniques that are used to remove toxins from the body. CHP generally is considered the preferred method for extracorporeal extraction of several toxins—toxins that are adsorbed by activated charcoal. Assessments of the tannic acid's protective effects on ophidian poisoning are associated with the toxic proteins' precipitation by tannic acid. The challenge in treating a snakebite lies in removing the injected poison with minimal damage to blood constituent proteins. An alternative is CHP, and this investigation proposed to develop a column for hemoperfuser cartridge, combining charcoal granules trapped between layers of polymeric material conjugated to tannic acid, using an in vitro model scaled to the Wistar rat, which can be tested in an animal model. The cartridge was evaluated using the 22 full factorial design, in duplicate, as a method to study the effects of granulated-charcoal size and tannic acid concentration on the hematologic profile (platelet and leukocyte counts) and biochemical profile (total serum protein and albumin dosages) of sheep blood. The results demonstrate that charcoal in hemoperfuser cartridge: (1) decreases the serum in sheep blood volume, as consequence, (2) increases the serum proteins' concentration, and (iii) exerts slight influence on albumin. The inclusion of tannic acid in hemoperfuser column precipitates some of serum proteins and albumin, decreasing their concentrations in the plasma serum. In conclusion, based on these effects we can suggest the use of 0.02 g tannic acid concentration and 8–20 mesh granulated charcoal in hemoperfuser cartridge for precipitating toxic proteins from snake venoms. |
topic |
Charcoal granules Hemoperfusion Snake venom Tannic acid |
url |
https://rabm.scholasticahq.com/article/900-development-and-preliminary-assessment-of-hemoperfusion-cartridge-with-tannic-acid-for-toxic-proteins-precipitation-an-in-vitro-model |
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