A GAL4-Driver Line Resource for Drosophila Neurobiology
We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expres...
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Language: | English |
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Elsevier
2012-10-01
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Series: | Cell Reports |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124712002926 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Arnim Jenett Gerald M. Rubin Teri-T.B. Ngo David Shepherd Christine Murphy Heather Dionne Barret D. Pfeiffer Amanda Cavallaro Donald Hall Jennifer Jeter Nirmala Iyer Dona Fetter Joanna H. Hausenfluck Hanchuan Peng Eric T. Trautman Robert R. Svirskas Eugene W. Myers Zbigniew R. Iwinski Yoshinori Aso Gina M. DePasquale Adrianne Enos Phuson Hulamm Shing Chun Benny Lam Hsing-Hsi Li Todd R. Laverty Fuhui Long Lei Qu Sean D. Murphy Konrad Rokicki Todd Safford Kshiti Shaw Julie H. Simpson Allison Sowell Susana Tae Yang Yu Christopher T. Zugates |
spellingShingle |
Arnim Jenett Gerald M. Rubin Teri-T.B. Ngo David Shepherd Christine Murphy Heather Dionne Barret D. Pfeiffer Amanda Cavallaro Donald Hall Jennifer Jeter Nirmala Iyer Dona Fetter Joanna H. Hausenfluck Hanchuan Peng Eric T. Trautman Robert R. Svirskas Eugene W. Myers Zbigniew R. Iwinski Yoshinori Aso Gina M. DePasquale Adrianne Enos Phuson Hulamm Shing Chun Benny Lam Hsing-Hsi Li Todd R. Laverty Fuhui Long Lei Qu Sean D. Murphy Konrad Rokicki Todd Safford Kshiti Shaw Julie H. Simpson Allison Sowell Susana Tae Yang Yu Christopher T. Zugates A GAL4-Driver Line Resource for Drosophila Neurobiology Cell Reports |
author_facet |
Arnim Jenett Gerald M. Rubin Teri-T.B. Ngo David Shepherd Christine Murphy Heather Dionne Barret D. Pfeiffer Amanda Cavallaro Donald Hall Jennifer Jeter Nirmala Iyer Dona Fetter Joanna H. Hausenfluck Hanchuan Peng Eric T. Trautman Robert R. Svirskas Eugene W. Myers Zbigniew R. Iwinski Yoshinori Aso Gina M. DePasquale Adrianne Enos Phuson Hulamm Shing Chun Benny Lam Hsing-Hsi Li Todd R. Laverty Fuhui Long Lei Qu Sean D. Murphy Konrad Rokicki Todd Safford Kshiti Shaw Julie H. Simpson Allison Sowell Susana Tae Yang Yu Christopher T. Zugates |
author_sort |
Arnim Jenett |
title |
A GAL4-Driver Line Resource for Drosophila Neurobiology |
title_short |
A GAL4-Driver Line Resource for Drosophila Neurobiology |
title_full |
A GAL4-Driver Line Resource for Drosophila Neurobiology |
title_fullStr |
A GAL4-Driver Line Resource for Drosophila Neurobiology |
title_full_unstemmed |
A GAL4-Driver Line Resource for Drosophila Neurobiology |
title_sort |
gal4-driver line resource for drosophila neurobiology |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2012-10-01 |
description |
We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.
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url |
http://www.sciencedirect.com/science/article/pii/S2211124712002926 |
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doaj-dbd0a912927e488f9befcefdee9e79e02020-11-24T21:54:51ZengElsevierCell Reports2211-12472012-10-0124991100110.1016/j.celrep.2012.09.011A GAL4-Driver Line Resource for Drosophila NeurobiologyArnim Jenett0Gerald M. Rubin1Teri-T.B. Ngo2David Shepherd3Christine Murphy4Heather Dionne5Barret D. Pfeiffer6Amanda Cavallaro7Donald Hall8Jennifer Jeter9Nirmala Iyer10Dona Fetter11Joanna H. Hausenfluck12Hanchuan Peng13Eric T. Trautman14Robert R. Svirskas15Eugene W. Myers16Zbigniew R. Iwinski17Yoshinori Aso18Gina M. DePasquale19Adrianne Enos20Phuson Hulamm21Shing Chun Benny Lam22Hsing-Hsi Li23Todd R. Laverty24Fuhui Long25Lei Qu26Sean D. Murphy27Konrad Rokicki28Todd Safford29Kshiti Shaw30Julie H. Simpson31Allison Sowell32Susana Tae33Yang Yu34Christopher T. Zugates35Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USASchool of Biological Sciences, Bangor University, Deiniol Road, Bangor LL57 2UW, UKJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USACarl Zeiss Microscopy, LLC, United States, 1 Zeiss Drive, Thornwood, NY 10594, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function. http://www.sciencedirect.com/science/article/pii/S2211124712002926 |