Associations between phenotypic characteristics and clinical parameters of broilers and intestinal microbial development throughout a production cycle: A field study
Abstract Disturbances in intestinal health are a common problem affecting commercial broiler chickens worldwide. Several studies have revealed associations between health, production performance, and intestinal microbiota. This study aimed to describe the development of the intestinal microbiota of...
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doaj-dbd5d303a9b34854bc209b9cb50369312020-11-25T04:07:54ZengWileyMicrobiologyOpen2045-88272020-11-01911n/an/a10.1002/mbo3.1114Associations between phenotypic characteristics and clinical parameters of broilers and intestinal microbial development throughout a production cycle: A field studyJannigje G. Kers0Jean E. deOliveira1Egil A. J. Fischer2Monique H. G. Tersteeg‐Zijderveld3Prokopis Konstanti4Jan Arend (Arjan) Stegeman5Hauke Smidt6Francisca C. Velkers7Department of Population Health Sciences Division of Farm Animal Health Faculty of Veterinary Medicine Utrecht University Utrecht The NetherlandsCargill Animal Nutrition and Health Innovation Center Velddriel Velddriel The NetherlandsDepartment of Population Health Sciences Division of Farm Animal Health Faculty of Veterinary Medicine Utrecht University Utrecht The NetherlandsDepartment of Population Health Sciences Institute for Risk Assessment Sciences Faculty of Veterinary Medicine Utrecht University Utrecht The NetherlandsLaboratory of Microbiology Wageningen University & Research Wageningen The NetherlandsDepartment of Population Health Sciences Division of Farm Animal Health Faculty of Veterinary Medicine Utrecht University Utrecht The NetherlandsLaboratory of Microbiology Wageningen University & Research Wageningen The NetherlandsDepartment of Population Health Sciences Division of Farm Animal Health Faculty of Veterinary Medicine Utrecht University Utrecht The NetherlandsAbstract Disturbances in intestinal health are a common problem affecting commercial broiler chickens worldwide. Several studies have revealed associations between health, production performance, and intestinal microbiota. This study aimed to describe the development of the intestinal microbiota of broilers within a production cycle to evaluate to what extent clinical parameters and phenotypic characteristics can explain the intestinal microbiota variation. Of four well‐performing flocks within two farms, the cecal content was collected of nine broilers at 0, 2, 4, or 5, 7, 11, or 12, 14, 21, 28, 35, and 40 days of the production cycle. In total, 342 samples were analyzed using 16S ribosomal RNA gene amplicon sequencing. Variables as macroscopic gut abnormalities, gut lesions, age, individual body weight, sex, footpad integrity, the color of ceca, and foam in cecal content were determined. Ileum tissue was collected for histological quantification of villus length and crypt depth. Flock infection levels of the intestinal disease coccidiosis were measured in pooled feces from the poultry house. Increases in phylogenetic diversity were observed from hatch until day 21 of age. Constrained multivariate analysis indicated that age, farm, body weight, ileum crypt depth, cecal color, and the coccidiosis lesion score were important variables to describe the variation in cecal microbiota. These results contribute to determining relevant variables in flocks that may be indicative of the intestinal microbiota composition. Moreover, this knowledge increases the awareness of interactions between the intestinal microbiota and broiler health as well as their relative importance.https://doi.org/10.1002/mbo3.111416S rRNA genececal microbiotafield studyphenotypespoultry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jannigje G. Kers Jean E. deOliveira Egil A. J. Fischer Monique H. G. Tersteeg‐Zijderveld Prokopis Konstanti Jan Arend (Arjan) Stegeman Hauke Smidt Francisca C. Velkers |
spellingShingle |
Jannigje G. Kers Jean E. deOliveira Egil A. J. Fischer Monique H. G. Tersteeg‐Zijderveld Prokopis Konstanti Jan Arend (Arjan) Stegeman Hauke Smidt Francisca C. Velkers Associations between phenotypic characteristics and clinical parameters of broilers and intestinal microbial development throughout a production cycle: A field study MicrobiologyOpen 16S rRNA gene cecal microbiota field study phenotypes poultry |
author_facet |
Jannigje G. Kers Jean E. deOliveira Egil A. J. Fischer Monique H. G. Tersteeg‐Zijderveld Prokopis Konstanti Jan Arend (Arjan) Stegeman Hauke Smidt Francisca C. Velkers |
author_sort |
Jannigje G. Kers |
title |
Associations between phenotypic characteristics and clinical parameters of broilers and intestinal microbial development throughout a production cycle: A field study |
title_short |
Associations between phenotypic characteristics and clinical parameters of broilers and intestinal microbial development throughout a production cycle: A field study |
title_full |
Associations between phenotypic characteristics and clinical parameters of broilers and intestinal microbial development throughout a production cycle: A field study |
title_fullStr |
Associations between phenotypic characteristics and clinical parameters of broilers and intestinal microbial development throughout a production cycle: A field study |
title_full_unstemmed |
Associations between phenotypic characteristics and clinical parameters of broilers and intestinal microbial development throughout a production cycle: A field study |
title_sort |
associations between phenotypic characteristics and clinical parameters of broilers and intestinal microbial development throughout a production cycle: a field study |
publisher |
Wiley |
series |
MicrobiologyOpen |
issn |
2045-8827 |
publishDate |
2020-11-01 |
description |
Abstract Disturbances in intestinal health are a common problem affecting commercial broiler chickens worldwide. Several studies have revealed associations between health, production performance, and intestinal microbiota. This study aimed to describe the development of the intestinal microbiota of broilers within a production cycle to evaluate to what extent clinical parameters and phenotypic characteristics can explain the intestinal microbiota variation. Of four well‐performing flocks within two farms, the cecal content was collected of nine broilers at 0, 2, 4, or 5, 7, 11, or 12, 14, 21, 28, 35, and 40 days of the production cycle. In total, 342 samples were analyzed using 16S ribosomal RNA gene amplicon sequencing. Variables as macroscopic gut abnormalities, gut lesions, age, individual body weight, sex, footpad integrity, the color of ceca, and foam in cecal content were determined. Ileum tissue was collected for histological quantification of villus length and crypt depth. Flock infection levels of the intestinal disease coccidiosis were measured in pooled feces from the poultry house. Increases in phylogenetic diversity were observed from hatch until day 21 of age. Constrained multivariate analysis indicated that age, farm, body weight, ileum crypt depth, cecal color, and the coccidiosis lesion score were important variables to describe the variation in cecal microbiota. These results contribute to determining relevant variables in flocks that may be indicative of the intestinal microbiota composition. Moreover, this knowledge increases the awareness of interactions between the intestinal microbiota and broiler health as well as their relative importance. |
topic |
16S rRNA gene cecal microbiota field study phenotypes poultry |
url |
https://doi.org/10.1002/mbo3.1114 |
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