High Fat Mixed Meal Tolerance Test Leads to Suppression of Osteocalcin Decrease in Obese Insulin Resistant Subjects Compared to Healthy Adults
Nutrients influence bone turnover. Carboxylated osteocalcin (Gla-OC) participates in bone formation whereas its undercarboxylated form (Glu-OC) acts as a hormone in glucose metabolism. The aim of the study was to determine the responses of Gla-OC, Glu-OC, and total-OC (calculated as the sum of Gla-O...
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doaj-dbece8bafbf04d00b81a976d08bdeda62020-11-24T20:44:35ZengMDPI AGNutrients2072-66432018-11-011011161110.3390/nu10111611nu10111611High Fat Mixed Meal Tolerance Test Leads to Suppression of Osteocalcin Decrease in Obese Insulin Resistant Subjects Compared to Healthy AdultsUrszula Razny0Joanna Goralska1Anna Zdzienicka2Anna Gruca3Barbara Zapala4Agnieszka Micek5Aldona Dembinska-Kiec6Bogdan Solnica7Malgorzata Malczewska-Malec8Department of Clinical Biochemistry, Jagiellonian University Medical College, Kopernika 15a, 31-501 Krakow, PolandDepartment of Clinical Biochemistry, Jagiellonian University Medical College, Kopernika 15a, 31-501 Krakow, PolandDepartment of Clinical Biochemistry, Jagiellonian University Medical College, Kopernika 15a, 31-501 Krakow, PolandDepartment of Clinical Biochemistry, Jagiellonian University Medical College, Kopernika 15a, 31-501 Krakow, PolandDepartment of Clinical Biochemistry, Jagiellonian University Medical College, Kopernika 15a, 31-501 Krakow, PolandDepartment of Nursing Management and Epidemiology Nursing, Institute of Nursing and Midwifery, Jagiellonian University Medical College, Kopernika 25, 31-501 Krakow, PolandDepartment of Clinical Biochemistry, Jagiellonian University Medical College, Kopernika 15a, 31-501 Krakow, PolandDepartment of Clinical Biochemistry, Jagiellonian University Medical College, Kopernika 15a, 31-501 Krakow, PolandDepartment of Clinical Biochemistry, Jagiellonian University Medical College, Kopernika 15a, 31-501 Krakow, PolandNutrients influence bone turnover. Carboxylated osteocalcin (Gla-OC) participates in bone formation whereas its undercarboxylated form (Glu-OC) acts as a hormone in glucose metabolism. The aim of the study was to determine the responses of Gla-OC, Glu-OC, and total-OC (calculated as the sum of Gla-OC and Glu-OC) to a high fat mixed meal tolerance test (HFMTT) in non-obese (body mass index (BMI) < 30 kg/m<sup>2</sup>, <i>n</i> = 24) and obese subjects (30 < BMI < 40 kg/m<sup>2</sup>, <i>n</i> = 70) (both sexes, aged 25⁻65 years). Serum Gla-OC and Glu-OC were measured at baseline as well as at 2 and 6 h during a HFMTT by enzyme-linked immunosorbent assay (ELISA). Baseline Gla-OC, Glu-OC, and total-OC levels were lower in obese individuals compared to non-obese participants (<i>p</i> = 0.037, <i>p</i> = 0.016 and <i>p</i> = 0.005, respectively). The decrease in Gla-OC and total-OC, but not in Glu-OC, concentrations during the HFMTT was suppressed in obese, but not in non-obese controls (<i>p</i> < 0.05, <i>p</i> < 0.01, <i>p</i> = 0.08, respectively). Subjects with the highest homeostatic model assessment for insulin resistance (HOMA-IR) index values had a less pronounced decrease in total-OC compared to patients with values of HOMA-IR index in the 1st quartile (<i>p</i> < 0.05). Net incremental area under Gla-OC inversely correlated with adiponectin (rho = −0.35, <i>p</i> = 0.001). Increase in insulin sensitivity and adiponectin level in obese subjects could beneficially influence postprandial bone turnover expressed by osteocalcin concentration.https://www.mdpi.com/2072-6643/10/11/1611Bone formation in obesityinsulin resistanceosteocalcinmixed meal tolerance testpostprandial bone turnover |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Urszula Razny Joanna Goralska Anna Zdzienicka Anna Gruca Barbara Zapala Agnieszka Micek Aldona Dembinska-Kiec Bogdan Solnica Malgorzata Malczewska-Malec |
spellingShingle |
Urszula Razny Joanna Goralska Anna Zdzienicka Anna Gruca Barbara Zapala Agnieszka Micek Aldona Dembinska-Kiec Bogdan Solnica Malgorzata Malczewska-Malec High Fat Mixed Meal Tolerance Test Leads to Suppression of Osteocalcin Decrease in Obese Insulin Resistant Subjects Compared to Healthy Adults Nutrients Bone formation in obesity insulin resistance osteocalcin mixed meal tolerance test postprandial bone turnover |
author_facet |
Urszula Razny Joanna Goralska Anna Zdzienicka Anna Gruca Barbara Zapala Agnieszka Micek Aldona Dembinska-Kiec Bogdan Solnica Malgorzata Malczewska-Malec |
author_sort |
Urszula Razny |
title |
High Fat Mixed Meal Tolerance Test Leads to Suppression of Osteocalcin Decrease in Obese Insulin Resistant Subjects Compared to Healthy Adults |
title_short |
High Fat Mixed Meal Tolerance Test Leads to Suppression of Osteocalcin Decrease in Obese Insulin Resistant Subjects Compared to Healthy Adults |
title_full |
High Fat Mixed Meal Tolerance Test Leads to Suppression of Osteocalcin Decrease in Obese Insulin Resistant Subjects Compared to Healthy Adults |
title_fullStr |
High Fat Mixed Meal Tolerance Test Leads to Suppression of Osteocalcin Decrease in Obese Insulin Resistant Subjects Compared to Healthy Adults |
title_full_unstemmed |
High Fat Mixed Meal Tolerance Test Leads to Suppression of Osteocalcin Decrease in Obese Insulin Resistant Subjects Compared to Healthy Adults |
title_sort |
high fat mixed meal tolerance test leads to suppression of osteocalcin decrease in obese insulin resistant subjects compared to healthy adults |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2018-11-01 |
description |
Nutrients influence bone turnover. Carboxylated osteocalcin (Gla-OC) participates in bone formation whereas its undercarboxylated form (Glu-OC) acts as a hormone in glucose metabolism. The aim of the study was to determine the responses of Gla-OC, Glu-OC, and total-OC (calculated as the sum of Gla-OC and Glu-OC) to a high fat mixed meal tolerance test (HFMTT) in non-obese (body mass index (BMI) < 30 kg/m<sup>2</sup>, <i>n</i> = 24) and obese subjects (30 < BMI < 40 kg/m<sup>2</sup>, <i>n</i> = 70) (both sexes, aged 25⁻65 years). Serum Gla-OC and Glu-OC were measured at baseline as well as at 2 and 6 h during a HFMTT by enzyme-linked immunosorbent assay (ELISA). Baseline Gla-OC, Glu-OC, and total-OC levels were lower in obese individuals compared to non-obese participants (<i>p</i> = 0.037, <i>p</i> = 0.016 and <i>p</i> = 0.005, respectively). The decrease in Gla-OC and total-OC, but not in Glu-OC, concentrations during the HFMTT was suppressed in obese, but not in non-obese controls (<i>p</i> < 0.05, <i>p</i> < 0.01, <i>p</i> = 0.08, respectively). Subjects with the highest homeostatic model assessment for insulin resistance (HOMA-IR) index values had a less pronounced decrease in total-OC compared to patients with values of HOMA-IR index in the 1st quartile (<i>p</i> < 0.05). Net incremental area under Gla-OC inversely correlated with adiponectin (rho = −0.35, <i>p</i> = 0.001). Increase in insulin sensitivity and adiponectin level in obese subjects could beneficially influence postprandial bone turnover expressed by osteocalcin concentration. |
topic |
Bone formation in obesity insulin resistance osteocalcin mixed meal tolerance test postprandial bone turnover |
url |
https://www.mdpi.com/2072-6643/10/11/1611 |
work_keys_str_mv |
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