Triazole-Resistance in Environmental <i>Aspergillus fumigatus</i> in Latin American and African Countries
Triazole-resistance has been reported increasingly in <i>Aspergillus fumigatus</i>. An international expert team proposed to avoid triazole monotherapy for the initial treatment of invasive aspergillosis in regions with >10% environmental-resistance, but this prevalence is largely unk...
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doaj-dbfd9964650d4ca099f6ccc44684befe2021-04-12T23:04:54ZengMDPI AGJournal of Fungi2309-608X2021-04-01729229210.3390/jof7040292Triazole-Resistance in Environmental <i>Aspergillus fumigatus</i> in Latin American and African CountriesAgustin Resendiz-Sharpe0Klaas Dewaele1Rita Merckx2Beatriz Bustamante3Maria Celeste Vega-Gomez4Miriam Rolon5Jan Jacobs6Paul E. Verweij7Johan Maertens8Katrien Lagrou9Department of Microbiology, Immunology and Transplantation, KU Leuven, 3000 Leuven, BelgiumExcellence Center for Medical Mycology (ECMM), Department of Laboratory Medicine and National Reference Center for Mycosis, University Hospitals Leuven, 3000 Leuven, BelgiumDepartment of Microbiology, Immunology and Transplantation, KU Leuven, 3000 Leuven, BelgiumInstituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima 15102, PeruCentro para el Desarrollo de la Investigación Científica, CEDIC, Asunción 1255, ParaguayCentro para el Desarrollo de la Investigación Científica, CEDIC, Asunción 1255, ParaguayDepartment of Microbiology, Immunology and Transplantation, KU Leuven, 3000 Leuven, BelgiumDepartment of Medical Microbiology, Radboud University Medical Center, 6500 HB Nijmegen, The NetherlandsDepartment of Microbiology, Immunology and Transplantation, KU Leuven, 3000 Leuven, BelgiumDepartment of Microbiology, Immunology and Transplantation, KU Leuven, 3000 Leuven, BelgiumTriazole-resistance has been reported increasingly in <i>Aspergillus fumigatus</i>. An international expert team proposed to avoid triazole monotherapy for the initial treatment of invasive aspergillosis in regions with >10% environmental-resistance, but this prevalence is largely unknown for most American and African countries. Here, we screened 584 environmental samples (soil) from urban and rural locations in Mexico, Paraguay, and Peru in Latin America and Benin and Nigeria in Africa for triazole-resistant <i>A. fumigatus</i>. Samples were screened using triazole-containing agars and confirmed as triazole-resistant by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) broth dilution reference method. Isolates were further characterized by <i>cyp51A </i>sequencing and short-tandem repeat typing. Fungicide presence in samples was likewise determined. Among <i>A. fumigatus</i> positive samples, triazole-resistance was detected in 6.9% (7/102) of samples in Mexico, 8.3% (3/36) in Paraguay, 9.8% (6/61) in Peru, 2.2% (1/46) in Nigeria, and none in Benin. <i>C</i><i>yp51A</i> gene mutations were present in most of the triazole-resistant isolates (88%; 15/17). The environmentally-associated mutations TR<sub>34</sub>/L98H and TR<sub>46</sub>/Y121F/T289A were prevalent in Mexico and Peru, and isolates harboring these mutations were closely related. For the first time, triazole-resistant <i>A. fumigatus</i> was found in environmental samples in Mexico, Paraguay, Peru, and Nigeria with a prevalence of 7–10% in the Latin American countries. Our findings emphasize the need to establish triazole-resistance surveillance programs in these countries.https://www.mdpi.com/2309-608X/7/4/292<i>Aspergillus fumigatus</i>antifungal resistanceepidemiologyenvironmentAmericaAfrica |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Agustin Resendiz-Sharpe Klaas Dewaele Rita Merckx Beatriz Bustamante Maria Celeste Vega-Gomez Miriam Rolon Jan Jacobs Paul E. Verweij Johan Maertens Katrien Lagrou |
spellingShingle |
Agustin Resendiz-Sharpe Klaas Dewaele Rita Merckx Beatriz Bustamante Maria Celeste Vega-Gomez Miriam Rolon Jan Jacobs Paul E. Verweij Johan Maertens Katrien Lagrou Triazole-Resistance in Environmental <i>Aspergillus fumigatus</i> in Latin American and African Countries Journal of Fungi <i>Aspergillus fumigatus</i> antifungal resistance epidemiology environment America Africa |
author_facet |
Agustin Resendiz-Sharpe Klaas Dewaele Rita Merckx Beatriz Bustamante Maria Celeste Vega-Gomez Miriam Rolon Jan Jacobs Paul E. Verweij Johan Maertens Katrien Lagrou |
author_sort |
Agustin Resendiz-Sharpe |
title |
Triazole-Resistance in Environmental <i>Aspergillus fumigatus</i> in Latin American and African Countries |
title_short |
Triazole-Resistance in Environmental <i>Aspergillus fumigatus</i> in Latin American and African Countries |
title_full |
Triazole-Resistance in Environmental <i>Aspergillus fumigatus</i> in Latin American and African Countries |
title_fullStr |
Triazole-Resistance in Environmental <i>Aspergillus fumigatus</i> in Latin American and African Countries |
title_full_unstemmed |
Triazole-Resistance in Environmental <i>Aspergillus fumigatus</i> in Latin American and African Countries |
title_sort |
triazole-resistance in environmental <i>aspergillus fumigatus</i> in latin american and african countries |
publisher |
MDPI AG |
series |
Journal of Fungi |
issn |
2309-608X |
publishDate |
2021-04-01 |
description |
Triazole-resistance has been reported increasingly in <i>Aspergillus fumigatus</i>. An international expert team proposed to avoid triazole monotherapy for the initial treatment of invasive aspergillosis in regions with >10% environmental-resistance, but this prevalence is largely unknown for most American and African countries. Here, we screened 584 environmental samples (soil) from urban and rural locations in Mexico, Paraguay, and Peru in Latin America and Benin and Nigeria in Africa for triazole-resistant <i>A. fumigatus</i>. Samples were screened using triazole-containing agars and confirmed as triazole-resistant by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) broth dilution reference method. Isolates were further characterized by <i>cyp51A </i>sequencing and short-tandem repeat typing. Fungicide presence in samples was likewise determined. Among <i>A. fumigatus</i> positive samples, triazole-resistance was detected in 6.9% (7/102) of samples in Mexico, 8.3% (3/36) in Paraguay, 9.8% (6/61) in Peru, 2.2% (1/46) in Nigeria, and none in Benin. <i>C</i><i>yp51A</i> gene mutations were present in most of the triazole-resistant isolates (88%; 15/17). The environmentally-associated mutations TR<sub>34</sub>/L98H and TR<sub>46</sub>/Y121F/T289A were prevalent in Mexico and Peru, and isolates harboring these mutations were closely related. For the first time, triazole-resistant <i>A. fumigatus</i> was found in environmental samples in Mexico, Paraguay, Peru, and Nigeria with a prevalence of 7–10% in the Latin American countries. Our findings emphasize the need to establish triazole-resistance surveillance programs in these countries. |
topic |
<i>Aspergillus fumigatus</i> antifungal resistance epidemiology environment America Africa |
url |
https://www.mdpi.com/2309-608X/7/4/292 |
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