Overview of the most commonly used methods in allergen characterization

• Main Authors: TANJA CIRKOVIC VELICKOVIC, MARIJA GAVROVIC-JANKULOVIC, RATKO M. JANKOV
• Format: Article
• Language: English
• Published: Serbian Chemical Society 2005-03-01
• Series: Journal of the Serbian Chemical Society
• Subjects: allergen; characterization; allergy; immunoglobulin E; recombinant allergen.
• Online Access: http://www.shd.org.yu/HtDocs/SHD/vol70/No3/JSCS_V70_No3-04.pdf

The characterization of an allergen is a troublesome and difficult process, as it requires both the precise biochemical characterization of a (glyco)protein molecule and the establishment of its susceptibility to IgE antibodies, as they are themain link to histamine release in some hypersensitivity states (type I allergies). As the characterization of an allergen includes molecular weight determination of the allergenic molecule, its structure determination, physicochemical properties, IgE binding properties of the allergen molecule, and its allergenicity, an overal review of which biochemical and immunochemical methods are used in achieving this goal are presented in this paper. The information on the molecular level on the stuctures of allergens indicates that allergens are considerably heterogeneous protein structures, and that there is no particular aminoacid sequence which is responsible for the allergenicity. Therefore, information gained from detailed structural, functional and immunochemical studies of these intriguing molecules, which nowadaysmodulate a variety of pathophysiological conditions, would greatly improve our understanding of the underlying disease mechanisms, and the way to handle them.