Alternative Promoter Use Governs the Expression of IgLON Cell Adhesion Molecules in Histogenetic Fields of the Embryonic Mouse Brain
The members of the IgLON superfamily of cell adhesion molecules facilitate fundamental cellular communication during brain development, maintain functional brain circuitry, and are associated with several neuropsychiatric disorders such as depression, autism, schizophrenia, and intellectual disabili...
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doaj-dc31f81548de4161a3adab982e51b14b2021-07-15T15:37:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-01226955695510.3390/ijms22136955Alternative Promoter Use Governs the Expression of IgLON Cell Adhesion Molecules in Histogenetic Fields of the Embryonic Mouse BrainToomas Jagomäe0Katyayani Singh1Mari-Anne Philips2Mohan Jayaram3Kadri Seppa4Triin Tekko5Scott F. Gilbert6Eero Vasar7Kersti Lilleväli8Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 19 Ravila Street, 50411 Tartu, EstoniaDepartment of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 19 Ravila Street, 50411 Tartu, EstoniaDepartment of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 19 Ravila Street, 50411 Tartu, EstoniaDepartment of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 19 Ravila Street, 50411 Tartu, EstoniaDepartment of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 19 Ravila Street, 50411 Tartu, EstoniaThe Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, 2780-156 Oeiras, PortugalDepartment of Biology, Swarthmore College, Swarthmore, PA 19081, USADepartment of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 19 Ravila Street, 50411 Tartu, EstoniaDepartment of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 19 Ravila Street, 50411 Tartu, EstoniaThe members of the IgLON superfamily of cell adhesion molecules facilitate fundamental cellular communication during brain development, maintain functional brain circuitry, and are associated with several neuropsychiatric disorders such as depression, autism, schizophrenia, and intellectual disabilities. Usage of alternative promoter-specific <i>1a</i> and <i>1b</i> mRNA isoforms in <i>Lsamp</i>, <i>Opcml</i>, <i>Ntm</i>, and the single promoter of <i>Negr1</i> in the mouse and human brain has been previously described. To determine the precise spatiotemporal expression dynamics of <i>Lsamp</i>, <i>Opcml</i>, <i>Ntm</i> isoforms, and <i>Negr1</i>, in the developing brain, we generated isoform-specific RNA probes and carried out in situ hybridization in the developing (embryonic, E10.5, E11.5, 13.5, 17; postnatal, P0) and adult mouse brains. We show that promoter-specific expression of IgLONs is established early during pallial development (at E10.5), where it remains throughout its differentiation through adulthood. In the diencephalon, midbrain, and hindbrain, strong expression patterns are initiated a few days later and begin fading after birth, being only faintly expressed during adulthood. Thus, the expression of specific IgLONs in the developing brain may provide the means for regionally specific functionality as well as for specific regional vulnerabilities. The current study will therefore improve the understanding of how IgLON genes are implicated in the development of neuropsychiatric disorders.https://www.mdpi.com/1422-0067/22/13/6955IgLON<i>Lsamp</i><i>Ntm</i><i>Opcml</i><i>Negr1</i>alternative promoter |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Toomas Jagomäe Katyayani Singh Mari-Anne Philips Mohan Jayaram Kadri Seppa Triin Tekko Scott F. Gilbert Eero Vasar Kersti Lilleväli |
spellingShingle |
Toomas Jagomäe Katyayani Singh Mari-Anne Philips Mohan Jayaram Kadri Seppa Triin Tekko Scott F. Gilbert Eero Vasar Kersti Lilleväli Alternative Promoter Use Governs the Expression of IgLON Cell Adhesion Molecules in Histogenetic Fields of the Embryonic Mouse Brain International Journal of Molecular Sciences IgLON <i>Lsamp</i> <i>Ntm</i> <i>Opcml</i> <i>Negr1</i> alternative promoter |
author_facet |
Toomas Jagomäe Katyayani Singh Mari-Anne Philips Mohan Jayaram Kadri Seppa Triin Tekko Scott F. Gilbert Eero Vasar Kersti Lilleväli |
author_sort |
Toomas Jagomäe |
title |
Alternative Promoter Use Governs the Expression of IgLON Cell Adhesion Molecules in Histogenetic Fields of the Embryonic Mouse Brain |
title_short |
Alternative Promoter Use Governs the Expression of IgLON Cell Adhesion Molecules in Histogenetic Fields of the Embryonic Mouse Brain |
title_full |
Alternative Promoter Use Governs the Expression of IgLON Cell Adhesion Molecules in Histogenetic Fields of the Embryonic Mouse Brain |
title_fullStr |
Alternative Promoter Use Governs the Expression of IgLON Cell Adhesion Molecules in Histogenetic Fields of the Embryonic Mouse Brain |
title_full_unstemmed |
Alternative Promoter Use Governs the Expression of IgLON Cell Adhesion Molecules in Histogenetic Fields of the Embryonic Mouse Brain |
title_sort |
alternative promoter use governs the expression of iglon cell adhesion molecules in histogenetic fields of the embryonic mouse brain |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-06-01 |
description |
The members of the IgLON superfamily of cell adhesion molecules facilitate fundamental cellular communication during brain development, maintain functional brain circuitry, and are associated with several neuropsychiatric disorders such as depression, autism, schizophrenia, and intellectual disabilities. Usage of alternative promoter-specific <i>1a</i> and <i>1b</i> mRNA isoforms in <i>Lsamp</i>, <i>Opcml</i>, <i>Ntm</i>, and the single promoter of <i>Negr1</i> in the mouse and human brain has been previously described. To determine the precise spatiotemporal expression dynamics of <i>Lsamp</i>, <i>Opcml</i>, <i>Ntm</i> isoforms, and <i>Negr1</i>, in the developing brain, we generated isoform-specific RNA probes and carried out in situ hybridization in the developing (embryonic, E10.5, E11.5, 13.5, 17; postnatal, P0) and adult mouse brains. We show that promoter-specific expression of IgLONs is established early during pallial development (at E10.5), where it remains throughout its differentiation through adulthood. In the diencephalon, midbrain, and hindbrain, strong expression patterns are initiated a few days later and begin fading after birth, being only faintly expressed during adulthood. Thus, the expression of specific IgLONs in the developing brain may provide the means for regionally specific functionality as well as for specific regional vulnerabilities. The current study will therefore improve the understanding of how IgLON genes are implicated in the development of neuropsychiatric disorders. |
topic |
IgLON <i>Lsamp</i> <i>Ntm</i> <i>Opcml</i> <i>Negr1</i> alternative promoter |
url |
https://www.mdpi.com/1422-0067/22/13/6955 |
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