Effect of Melatonin on the Renin-Angiotensin-Aldosterone System in l-NAME-Induced Hypertension
The renin-angiotensin-aldosterone system (RAAS) is a dominant player in several cardiovascular pathologies. This study investigated whether alterations induced by l-NAME, (NLG)-nitro-l-arginine methyl ester, a nitric oxide synthase inhibitor, and the protective effect of melatonin are associated wit...
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doaj-dc449466bd2141c89719c0b4a41f4ebf2020-11-24T21:42:14ZengMDPI AGMolecules1420-30492018-01-0123226510.3390/molecules23020265molecules23020265Effect of Melatonin on the Renin-Angiotensin-Aldosterone System in l-NAME-Induced HypertensionFedor Simko0Tomas Baka1Kristina Krajcirovicova2Kristina Repova3Silvia Aziriova4Stefan Zorad5Marko Poglitsch6Michaela Adamcova7Russel J. Reiter8Ludovit Paulis9Institute of Pathophysiology, Faculty of Medicine, Comenius University, Sasinkova 4, 81108 Bratislava, SlovakiaInstitute of Pathophysiology, Faculty of Medicine, Comenius University, Sasinkova 4, 81108 Bratislava, SlovakiaInstitute of Pathophysiology, Faculty of Medicine, Comenius University, Sasinkova 4, 81108 Bratislava, SlovakiaInstitute of Pathophysiology, Faculty of Medicine, Comenius University, Sasinkova 4, 81108 Bratislava, SlovakiaInstitute of Pathophysiology, Faculty of Medicine, Comenius University, Sasinkova 4, 81108 Bratislava, SlovakiaInstitute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, 84505 Bratislava, SlovakiaAttoquant Diagnostics, 1030 Vienna, AustriaDepartment of Physiology, School of Medicine, Charles University, 50003 Hradec Kralove, Czech RepublicDepartment of Cellular and Structural Biology, UT Health Science Center, San Antonio, TX 78229, USAInstitute of Pathophysiology, Faculty of Medicine, Comenius University, Sasinkova 4, 81108 Bratislava, SlovakiaThe renin-angiotensin-aldosterone system (RAAS) is a dominant player in several cardiovascular pathologies. This study investigated whether alterations induced by l-NAME, (NLG)-nitro-l-arginine methyl ester, a nitric oxide synthase inhibitor, and the protective effect of melatonin are associated with changes in the RAAS. Four groups of 3-month-old male Wistar rats (n = 10) were treated as follows for four weeks: untreated controls, rats treated with melatonin (10 mg/kg/day), rats treated with l-NAME (40 mg/kg/day), and rats treated with l-NAME + melatonin. l-NAME administration led to hypertension and left ventricular (LV) fibrosis in terms of enhancement of soluble, insoluble and total collagen concentration and content. Melatonin reduced systolic blood pressure enhancement and lowered the concentration and content of insoluble and total collagen in the LV. The serum concentration of angiotensin (Ang) 1–8 (Ang II) and its downstream metabolites were reduced in the l-NAME group and remained unaltered by melatonin. The serum aldosterone level and its ratio to Ang II (AA2-ratio) were increased in the l-NAME group without being modified by melatonin. We conclude that l-NAME-hypertension is associated with reduced level of Ang II and its downstream metabolites and increased aldosterone concentration and AA2-ratio. Melatonin exerts its protective effect in l-NAME-induced hypertension without affecting RAAS.http://www.mdpi.com/1420-3049/23/2/265">l-NAMEfibrosismelatoninangiotensin IIangiotensin 1–7aldosterone |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fedor Simko Tomas Baka Kristina Krajcirovicova Kristina Repova Silvia Aziriova Stefan Zorad Marko Poglitsch Michaela Adamcova Russel J. Reiter Ludovit Paulis |
spellingShingle |
Fedor Simko Tomas Baka Kristina Krajcirovicova Kristina Repova Silvia Aziriova Stefan Zorad Marko Poglitsch Michaela Adamcova Russel J. Reiter Ludovit Paulis Effect of Melatonin on the Renin-Angiotensin-Aldosterone System in l-NAME-Induced Hypertension Molecules ">l-NAME fibrosis melatonin angiotensin II angiotensin 1–7 aldosterone |
author_facet |
Fedor Simko Tomas Baka Kristina Krajcirovicova Kristina Repova Silvia Aziriova Stefan Zorad Marko Poglitsch Michaela Adamcova Russel J. Reiter Ludovit Paulis |
author_sort |
Fedor Simko |
title |
Effect of Melatonin on the Renin-Angiotensin-Aldosterone System in l-NAME-Induced Hypertension |
title_short |
Effect of Melatonin on the Renin-Angiotensin-Aldosterone System in l-NAME-Induced Hypertension |
title_full |
Effect of Melatonin on the Renin-Angiotensin-Aldosterone System in l-NAME-Induced Hypertension |
title_fullStr |
Effect of Melatonin on the Renin-Angiotensin-Aldosterone System in l-NAME-Induced Hypertension |
title_full_unstemmed |
Effect of Melatonin on the Renin-Angiotensin-Aldosterone System in l-NAME-Induced Hypertension |
title_sort |
effect of melatonin on the renin-angiotensin-aldosterone system in l-name-induced hypertension |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-01-01 |
description |
The renin-angiotensin-aldosterone system (RAAS) is a dominant player in several cardiovascular pathologies. This study investigated whether alterations induced by l-NAME, (NLG)-nitro-l-arginine methyl ester, a nitric oxide synthase inhibitor, and the protective effect of melatonin are associated with changes in the RAAS. Four groups of 3-month-old male Wistar rats (n = 10) were treated as follows for four weeks: untreated controls, rats treated with melatonin (10 mg/kg/day), rats treated with l-NAME (40 mg/kg/day), and rats treated with l-NAME + melatonin. l-NAME administration led to hypertension and left ventricular (LV) fibrosis in terms of enhancement of soluble, insoluble and total collagen concentration and content. Melatonin reduced systolic blood pressure enhancement and lowered the concentration and content of insoluble and total collagen in the LV. The serum concentration of angiotensin (Ang) 1–8 (Ang II) and its downstream metabolites were reduced in the l-NAME group and remained unaltered by melatonin. The serum aldosterone level and its ratio to Ang II (AA2-ratio) were increased in the l-NAME group without being modified by melatonin. We conclude that l-NAME-hypertension is associated with reduced level of Ang II and its downstream metabolites and increased aldosterone concentration and AA2-ratio. Melatonin exerts its protective effect in l-NAME-induced hypertension without affecting RAAS. |
topic |
">l-NAME fibrosis melatonin angiotensin II angiotensin 1–7 aldosterone |
url |
http://www.mdpi.com/1420-3049/23/2/265 |
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