<b>Short-term storage of lebranche mullet <i>Mugil liza</i> (Valenciennes, 1836) semen <i>in natura</i> and diluted with CF-HBSS

• Main Authors: Caio Cesar França Magnotti, Jorgélia de Jesus Pinto Castro, Fabiola Santiago Pedrotti, Fábio Carneiro Sterzelecki, Eduardo Gomes Sanches, Vinicius Ronzani Cerqueira
• Format: Article
• Language: English
• Published: Editora da Universidade Estadual de Maringá (Eduem) 2018-04-01
• Series: Acta Scientiarum: Animal Sciences
• Subjects: gamete conservation; spermatozoa; aquaculture; marine fish farming; near threatened fish.
• Online Access: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/39563
• ISSN: 1807-8672

The present study aimed to evaluate the activation characteristics of the lebranche mullet spermatozoa in natura and diluted with CF-HBSS for 96h at 4±2°C. The semen was collected from eight wild fish in Florianópolis – SC (Brazil) (27°S) in May, during reproductive migration. Three pools of semen were divided into two treatments: in natura and diluted with CF-HBSS 1:3. The semen was activated with seawater (salinity of 34, pH 8.7 and 4±2°C) to determine: motility time, motility rate and sperm cell membrane integrity. Dilution with CF-HBSS 1:3 increased motility time of diluted semen (15-20% for 6, 12 and 18h). Motility rate was equal to fresh semen for up to 24h of refrigeration and it was 30% higher than in natura semen at 12, 18 and 24h. Cell membrane integrity was maintained in fresh semen up to 6h, and it was 15-20% higher than in natura semen from 6 to 48h (p < 0.05). The use of CF-HBSS as diluent in the ratio 1:3 is recommended for semen refrigeration of lebranche mullet.