Chromosome Missegregation in Single Human Oocytes Is Related to the Age and Gene Expression Profile
The growing trend for women to postpone childbearing has resulted in a dramatic increase in the incidence of aneuploid pregnancies. Despite the importance to human reproductive health, the events precipitating female age-related meiotic errors are poorly understood. To gain new insight into the mole...
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doaj-dc69821ee7ba4e95a3bfaefff680ad492020-11-25T03:29:28ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-03-01216193410.3390/ijms21061934ijms21061934Chromosome Missegregation in Single Human Oocytes Is Related to the Age and Gene Expression ProfileStefano Barone0Patrizia Sarogni1Roberto Valli2Maria Michela Pallotta3Gazzi Silvia4Annalisa Frattini5Abdul Waheed Khan6Erika Rapalini7Cristiana Parri8Antonio Musio9Centro Procreazione Assistita Ospedale Versilia, Unità Sanitaria Locale USL Toscana Nordovest, 55041 Lido di Camaiore, ItalyInstitute for Genetic and Biomedical Research (IRGB), National Research Council (CNR), 56124 Pisa, ItalyMedical Genetics and Applied Biology Unit, Department of Medicine and Surgery, University of Insubria, 21100 Varese, ItalyInstitute for Genetic and Biomedical Research (IRGB), National Research Council (CNR), 56124 Pisa, ItalyCentro Procreazione Assistita Ospedale Versilia, Unità Sanitaria Locale USL Toscana Nordovest, 55041 Lido di Camaiore, ItalyMedical Genetics and Applied Biology Unit, Department of Medicine and Surgery, University of Insubria, 21100 Varese, ItalyMedical Genetics and Applied Biology Unit, Department of Medicine and Surgery, University of Insubria, 21100 Varese, ItalyCentro Procreazione Assistita Ospedale Versilia, Unità Sanitaria Locale USL Toscana Nordovest, 55041 Lido di Camaiore, ItalyCentro Procreazione Assistita Ospedale Versilia, Unità Sanitaria Locale USL Toscana Nordovest, 55041 Lido di Camaiore, ItalyInstitute for Genetic and Biomedical Research (IRGB), National Research Council (CNR), 56124 Pisa, ItalyThe growing trend for women to postpone childbearing has resulted in a dramatic increase in the incidence of aneuploid pregnancies. Despite the importance to human reproductive health, the events precipitating female age-related meiotic errors are poorly understood. To gain new insight into the molecular basis of age-related chromosome missegregation in human oocytes, we combined the transcriptome profiles of twenty single oocytes (derived from females divided into two groups according to age <35 and ≥35 years) with their chromosome status obtained by array comparative genomic hybridization (aCGH). Furthermore, we compared the transcription profile of the single oocyte with the surrounding cumulus cells (CCs). RNA-seq data showed differences in gene expression between young and old oocytes. Dysregulated genes play a role in important biological processes such as gene transcription regulation, cytoskeleton organization, pathways related to RNA maturation and translation. The comparison of the transcription profile of the oocyte and the corresponding CCs highlighted the differential expression of genes belonging to the G protein-coupled receptor superfamily. Finally, we detected the loss of a X chromosome in two oocytes derived from women belonging to the ≥35 years age group. These aneuploidies may be caused by the detriment of REEP4, an endoplasmic reticulum protein, in women aged ≥35 years. Here we gained new insight into the complex regulatory circuit between the oocyte and the surrounding CCs and uncovered a new putative molecular basis of age-related chromosome missegregation in human oocytes.https://www.mdpi.com/1422-0067/21/6/1934ovarian agingcumulus cellsgene expression profilerna-seqchromosome aneuploidyarray cghgpcrsreep4 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stefano Barone Patrizia Sarogni Roberto Valli Maria Michela Pallotta Gazzi Silvia Annalisa Frattini Abdul Waheed Khan Erika Rapalini Cristiana Parri Antonio Musio |
spellingShingle |
Stefano Barone Patrizia Sarogni Roberto Valli Maria Michela Pallotta Gazzi Silvia Annalisa Frattini Abdul Waheed Khan Erika Rapalini Cristiana Parri Antonio Musio Chromosome Missegregation in Single Human Oocytes Is Related to the Age and Gene Expression Profile International Journal of Molecular Sciences ovarian aging cumulus cells gene expression profile rna-seq chromosome aneuploidy array cgh gpcrs reep4 |
author_facet |
Stefano Barone Patrizia Sarogni Roberto Valli Maria Michela Pallotta Gazzi Silvia Annalisa Frattini Abdul Waheed Khan Erika Rapalini Cristiana Parri Antonio Musio |
author_sort |
Stefano Barone |
title |
Chromosome Missegregation in Single Human Oocytes Is Related to the Age and Gene Expression Profile |
title_short |
Chromosome Missegregation in Single Human Oocytes Is Related to the Age and Gene Expression Profile |
title_full |
Chromosome Missegregation in Single Human Oocytes Is Related to the Age and Gene Expression Profile |
title_fullStr |
Chromosome Missegregation in Single Human Oocytes Is Related to the Age and Gene Expression Profile |
title_full_unstemmed |
Chromosome Missegregation in Single Human Oocytes Is Related to the Age and Gene Expression Profile |
title_sort |
chromosome missegregation in single human oocytes is related to the age and gene expression profile |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-03-01 |
description |
The growing trend for women to postpone childbearing has resulted in a dramatic increase in the incidence of aneuploid pregnancies. Despite the importance to human reproductive health, the events precipitating female age-related meiotic errors are poorly understood. To gain new insight into the molecular basis of age-related chromosome missegregation in human oocytes, we combined the transcriptome profiles of twenty single oocytes (derived from females divided into two groups according to age <35 and ≥35 years) with their chromosome status obtained by array comparative genomic hybridization (aCGH). Furthermore, we compared the transcription profile of the single oocyte with the surrounding cumulus cells (CCs). RNA-seq data showed differences in gene expression between young and old oocytes. Dysregulated genes play a role in important biological processes such as gene transcription regulation, cytoskeleton organization, pathways related to RNA maturation and translation. The comparison of the transcription profile of the oocyte and the corresponding CCs highlighted the differential expression of genes belonging to the G protein-coupled receptor superfamily. Finally, we detected the loss of a X chromosome in two oocytes derived from women belonging to the ≥35 years age group. These aneuploidies may be caused by the detriment of REEP4, an endoplasmic reticulum protein, in women aged ≥35 years. Here we gained new insight into the complex regulatory circuit between the oocyte and the surrounding CCs and uncovered a new putative molecular basis of age-related chromosome missegregation in human oocytes. |
topic |
ovarian aging cumulus cells gene expression profile rna-seq chromosome aneuploidy array cgh gpcrs reep4 |
url |
https://www.mdpi.com/1422-0067/21/6/1934 |
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