Superior Alignment of Human iPSC-Osteoblasts Associated with Focal Adhesion Formation Stimulated by Oriented Collagen Scaffold
Human-induced pluripotent stem cells (hiPSCs) can be applied in patient-specific cell therapy to regenerate lost tissue or organ function. Anisotropic control of the structural organization in the newly generated bone matrix is pivotal for functional reconstruction during bone tissue regeneration. R...
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doaj-dc6a8b78a3ad46deafca715e4ea071b62021-06-30T23:44:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-01226232623210.3390/ijms22126232Superior Alignment of Human iPSC-Osteoblasts Associated with Focal Adhesion Formation Stimulated by Oriented Collagen ScaffoldRyosuke Ozasa0Aira Matsugaki1Tadaaki Matsuzaka2Takuya Ishimoto3Hui-Suk Yun4Takayoshi Nakano5Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Suita 565-0871, JapanDivision of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Suita 565-0871, JapanDivision of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Suita 565-0871, JapanDivision of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Suita 565-0871, JapanDepartment of Advanced Biomaterials Research, Materials Processing Innovation Research Division, Korea Institute of Materials Science, Changwon 51508, KoreaDivision of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Suita 565-0871, JapanHuman-induced pluripotent stem cells (hiPSCs) can be applied in patient-specific cell therapy to regenerate lost tissue or organ function. Anisotropic control of the structural organization in the newly generated bone matrix is pivotal for functional reconstruction during bone tissue regeneration. Recently, we revealed that hiPSC-derived osteoblasts (hiPSC-Obs) exhibit preferential alignment and organize in highly ordered bone matrices along a bone-mimetic collagen scaffold, indicating their critical role in regulating the unidirectional cellular arrangement, as well as the structural organization of regenerated bone tissue. However, it remains unclear how hiPSCs exhibit the cell properties required for oriented tissue construction. The present study aimed to characterize the properties of hiPSCs-Obs and those of their focal adhesions (FAs), which mediate the structural relationship between cells and the matrix. Our in vitro anisotropic cell culture system revealed the superior adhesion behavior of hiPSC-Obs, which exhibited accelerated cell proliferation and better cell alignment along the collagen axis compared to normal human osteoblasts. Notably, the oriented collagen scaffold stimulated FA formation along the scaffold collagen orientation. This is the first report of the superior cell adhesion behavior of hiPSC-Obs associated with the promotion of FA assembly along an anisotropic scaffold. These findings suggest a promising role for hiPSCs in enabling anisotropic bone microstructural regeneration.https://www.mdpi.com/1422-0067/22/12/6232induced pluripotent stem cellcell therapybone regenerative medicinecell proliferationcellular arrangementosteoblast |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ryosuke Ozasa Aira Matsugaki Tadaaki Matsuzaka Takuya Ishimoto Hui-Suk Yun Takayoshi Nakano |
spellingShingle |
Ryosuke Ozasa Aira Matsugaki Tadaaki Matsuzaka Takuya Ishimoto Hui-Suk Yun Takayoshi Nakano Superior Alignment of Human iPSC-Osteoblasts Associated with Focal Adhesion Formation Stimulated by Oriented Collagen Scaffold International Journal of Molecular Sciences induced pluripotent stem cell cell therapy bone regenerative medicine cell proliferation cellular arrangement osteoblast |
author_facet |
Ryosuke Ozasa Aira Matsugaki Tadaaki Matsuzaka Takuya Ishimoto Hui-Suk Yun Takayoshi Nakano |
author_sort |
Ryosuke Ozasa |
title |
Superior Alignment of Human iPSC-Osteoblasts Associated with Focal Adhesion Formation Stimulated by Oriented Collagen Scaffold |
title_short |
Superior Alignment of Human iPSC-Osteoblasts Associated with Focal Adhesion Formation Stimulated by Oriented Collagen Scaffold |
title_full |
Superior Alignment of Human iPSC-Osteoblasts Associated with Focal Adhesion Formation Stimulated by Oriented Collagen Scaffold |
title_fullStr |
Superior Alignment of Human iPSC-Osteoblasts Associated with Focal Adhesion Formation Stimulated by Oriented Collagen Scaffold |
title_full_unstemmed |
Superior Alignment of Human iPSC-Osteoblasts Associated with Focal Adhesion Formation Stimulated by Oriented Collagen Scaffold |
title_sort |
superior alignment of human ipsc-osteoblasts associated with focal adhesion formation stimulated by oriented collagen scaffold |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-06-01 |
description |
Human-induced pluripotent stem cells (hiPSCs) can be applied in patient-specific cell therapy to regenerate lost tissue or organ function. Anisotropic control of the structural organization in the newly generated bone matrix is pivotal for functional reconstruction during bone tissue regeneration. Recently, we revealed that hiPSC-derived osteoblasts (hiPSC-Obs) exhibit preferential alignment and organize in highly ordered bone matrices along a bone-mimetic collagen scaffold, indicating their critical role in regulating the unidirectional cellular arrangement, as well as the structural organization of regenerated bone tissue. However, it remains unclear how hiPSCs exhibit the cell properties required for oriented tissue construction. The present study aimed to characterize the properties of hiPSCs-Obs and those of their focal adhesions (FAs), which mediate the structural relationship between cells and the matrix. Our in vitro anisotropic cell culture system revealed the superior adhesion behavior of hiPSC-Obs, which exhibited accelerated cell proliferation and better cell alignment along the collagen axis compared to normal human osteoblasts. Notably, the oriented collagen scaffold stimulated FA formation along the scaffold collagen orientation. This is the first report of the superior cell adhesion behavior of hiPSC-Obs associated with the promotion of FA assembly along an anisotropic scaffold. These findings suggest a promising role for hiPSCs in enabling anisotropic bone microstructural regeneration. |
topic |
induced pluripotent stem cell cell therapy bone regenerative medicine cell proliferation cellular arrangement osteoblast |
url |
https://www.mdpi.com/1422-0067/22/12/6232 |
work_keys_str_mv |
AT ryosukeozasa superioralignmentofhumanipscosteoblastsassociatedwithfocaladhesionformationstimulatedbyorientedcollagenscaffold AT airamatsugaki superioralignmentofhumanipscosteoblastsassociatedwithfocaladhesionformationstimulatedbyorientedcollagenscaffold AT tadaakimatsuzaka superioralignmentofhumanipscosteoblastsassociatedwithfocaladhesionformationstimulatedbyorientedcollagenscaffold AT takuyaishimoto superioralignmentofhumanipscosteoblastsassociatedwithfocaladhesionformationstimulatedbyorientedcollagenscaffold AT huisukyun superioralignmentofhumanipscosteoblastsassociatedwithfocaladhesionformationstimulatedbyorientedcollagenscaffold AT takayoshinakano superioralignmentofhumanipscosteoblastsassociatedwithfocaladhesionformationstimulatedbyorientedcollagenscaffold |
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