Extracellular Vesicle Quantification and Characterization: Common Methods and Emerging Approaches

Extracellular vesicles (EVs) are a family of small membrane vesicles that carry information about cells by which they are secreted. Growing interest in the role of EVs in intercellular communication, but also in using their diagnostic, prognostic and therapeutic potential in (bio) medical applicatio...

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Main Authors:	Thomas A. Hartjes, Serhii Mytnyk, Guido W. Jenster, Volkert van Steijn, Martin E. van Royen
Format:	Article
Language:	English
Published:	MDPI AG 2019-01-01
Series:	Bioengineering
Subjects:	Extracellular vesicles (EVs), methods for EV analysis emerging technologies
Online Access:	http://www.mdpi.com/2306-5354/6/1/7

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spelling	doaj-dc933d5b30aa4681a5cce47a99d0ad192020-11-25T01:38:23ZengMDPI AGBioengineering2306-53542019-01-0161710.3390/bioengineering6010007bioengineering6010007Extracellular Vesicle Quantification and Characterization: Common Methods and Emerging ApproachesThomas A. Hartjes0Serhii Mytnyk1Guido W. Jenster2Volkert van Steijn3Martin E. van Royen4Department of Pathology, Erasmus Optical Imaging Centre, Erasmus MC, 3015 GE Rotterdam, The NetherlandsDepartment of Chemical Engineering, Delft University of Technology, 3015 CD Delft, The NetherlandsDepartment of Urology, Erasmus MC, 3015 CD Rotterdam, The NetherlandsDepartment of Chemical Engineering, Delft University of Technology, 3015 CD Delft, The NetherlandsDepartment of Pathology, Erasmus Optical Imaging Centre, Erasmus MC, 3015 GE Rotterdam, The NetherlandsExtracellular vesicles (EVs) are a family of small membrane vesicles that carry information about cells by which they are secreted. Growing interest in the role of EVs in intercellular communication, but also in using their diagnostic, prognostic and therapeutic potential in (bio) medical applications, demands for accurate assessment of their biochemical and physical properties. In this review, we provide an overview of available technologies for EV analysis by describing their working principles, assessing their utility in EV research and summarising their potential and limitations. To emphasise the innovations in EV analysis, we also highlight the unique possibilities of emerging technologies with high potential for further development.http://www.mdpi.com/2306-5354/6/1/7Extracellular vesicles (EVs), methods for EV analysisemerging technologies
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Thomas A. Hartjes Serhii Mytnyk Guido W. Jenster Volkert van Steijn Martin E. van Royen
spellingShingle	Thomas A. Hartjes Serhii Mytnyk Guido W. Jenster Volkert van Steijn Martin E. van Royen Extracellular Vesicle Quantification and Characterization: Common Methods and Emerging Approaches Bioengineering Extracellular vesicles (EVs), methods for EV analysis emerging technologies
author_facet	Thomas A. Hartjes Serhii Mytnyk Guido W. Jenster Volkert van Steijn Martin E. van Royen
author_sort	Thomas A. Hartjes
title	Extracellular Vesicle Quantification and Characterization: Common Methods and Emerging Approaches
title_short	Extracellular Vesicle Quantification and Characterization: Common Methods and Emerging Approaches
title_full	Extracellular Vesicle Quantification and Characterization: Common Methods and Emerging Approaches
title_fullStr	Extracellular Vesicle Quantification and Characterization: Common Methods and Emerging Approaches
title_full_unstemmed	Extracellular Vesicle Quantification and Characterization: Common Methods and Emerging Approaches
title_sort	extracellular vesicle quantification and characterization: common methods and emerging approaches
publisher	MDPI AG
series	Bioengineering
issn	2306-5354
publishDate	2019-01-01
description	Extracellular vesicles (EVs) are a family of small membrane vesicles that carry information about cells by which they are secreted. Growing interest in the role of EVs in intercellular communication, but also in using their diagnostic, prognostic and therapeutic potential in (bio) medical applications, demands for accurate assessment of their biochemical and physical properties. In this review, we provide an overview of available technologies for EV analysis by describing their working principles, assessing their utility in EV research and summarising their potential and limitations. To emphasise the innovations in EV analysis, we also highlight the unique possibilities of emerging technologies with high potential for further development.
topic	Extracellular vesicles (EVs), methods for EV analysis emerging technologies
url	http://www.mdpi.com/2306-5354/6/1/7
work_keys_str_mv	AT thomasahartjes extracellularvesiclequantificationandcharacterizationcommonmethodsandemergingapproaches AT serhiimytnyk extracellularvesiclequantificationandcharacterizationcommonmethodsandemergingapproaches AT guidowjenster extracellularvesiclequantificationandcharacterizationcommonmethodsandemergingapproaches AT volkertvansteijn extracellularvesiclequantificationandcharacterizationcommonmethodsandemergingapproaches AT martinevanroyen extracellularvesiclequantificationandcharacterizationcommonmethodsandemergingapproaches
_version_	1725054101775974400

Extracellular Vesicle Quantification and Characterization: Common Methods and Emerging Approaches

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