Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis
Abstract Background Milk is a complex liquid that provides nutrition to newborns. Recent reports demonstrated that milk is enriched in maternal-derived exosomes that are involved in fetal physiological and pathological conditions by transmission of exosomal mRNAs, miRNAs and proteins. Until now, the...
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BMC
2017-04-01
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Series: | BMC Veterinary Research |
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Online Access: | http://link.springer.com/article/10.1186/s12917-017-1021-8 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ting Chen Qian-Yun Xi Jia-Jie Sun Rui-Song Ye Xiao Cheng Rui-Ping Sun Song-Bo Wang Gang Shu Li-Na Wang Xiao-Tong Zhu Qing-Yan Jiang Yong-Liang Zhang |
spellingShingle |
Ting Chen Qian-Yun Xi Jia-Jie Sun Rui-Song Ye Xiao Cheng Rui-Ping Sun Song-Bo Wang Gang Shu Li-Na Wang Xiao-Tong Zhu Qing-Yan Jiang Yong-Liang Zhang Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis BMC Veterinary Research Porcine milk exosomes RNA-seq Proteomic analysis |
author_facet |
Ting Chen Qian-Yun Xi Jia-Jie Sun Rui-Song Ye Xiao Cheng Rui-Ping Sun Song-Bo Wang Gang Shu Li-Na Wang Xiao-Tong Zhu Qing-Yan Jiang Yong-Liang Zhang |
author_sort |
Ting Chen |
title |
Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis |
title_short |
Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis |
title_full |
Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis |
title_fullStr |
Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis |
title_full_unstemmed |
Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis |
title_sort |
revelation of mrnas and proteins in porcine milk exosomes by transcriptomic and proteomic analysis |
publisher |
BMC |
series |
BMC Veterinary Research |
issn |
1746-6148 |
publishDate |
2017-04-01 |
description |
Abstract Background Milk is a complex liquid that provides nutrition to newborns. Recent reports demonstrated that milk is enriched in maternal-derived exosomes that are involved in fetal physiological and pathological conditions by transmission of exosomal mRNAs, miRNAs and proteins. Until now, there is no such research relevant to exosomal mRNAs and proteins in porcine milk, therefore, we have attempted to investigate porcine milk exosomal mRNAs and proteins using RNA-sequencing and proteomic analysis. Results A total of 16,304 (13,895 known and 2,409 novel mRNAs) mRNAs and 639 (571 known, 66 candidate and 2 putative proteins) proteins were identified. GO and KEGG annotation indicated that most proteins were located in the cytoplasm and participated in many immunity and disease-related pathways, and some mRNAs were closely related to metabolisms, degradation and signaling pathways. Interestingly, 19 categories of proteins were tissue-specific and detected in placenta, liver, milk, plasma and mammary. COG analysis divided the identified mRNAs and proteins into 6 and 23 categories, respectively, 18 mRNAs and 10 proteins appeared to be involved in cell cycle control, cell division and chromosome partitioning. Additionally, 14 selected mRNAs were identified by qPCR, meanwhile, 10 proteins related to immunity and cell proliferation were detected by Western blot. Conclusions These results provide the first insight into porcine milk exosomal mRNA and proteins, and will facilitate further research into the physiological significance of milk exosomes for infants. |
topic |
Porcine milk exosomes RNA-seq Proteomic analysis |
url |
http://link.springer.com/article/10.1186/s12917-017-1021-8 |
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doaj-dcb2f924563a40608e23d995a3ec5db02020-11-25T00:17:15ZengBMCBMC Veterinary Research1746-61482017-04-0113111410.1186/s12917-017-1021-8Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysisTing Chen0Qian-Yun Xi1Jia-Jie Sun2Rui-Song Ye3Xiao Cheng4Rui-Ping Sun5Song-Bo Wang6Gang Shu7Li-Na Wang8Xiao-Tong Zhu9Qing-Yan Jiang10Yong-Liang Zhang11National Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guandong Province Research Center of Woody Forage Engineering and Technology, South China Agricultural UniversityNational Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guandong Province Research Center of Woody Forage Engineering and Technology, South China Agricultural UniversityNational Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guandong Province Research Center of Woody Forage Engineering and Technology, South China Agricultural UniversityNational Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guandong Province Research Center of Woody Forage Engineering and Technology, South China Agricultural UniversityNational Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guandong Province Research Center of Woody Forage Engineering and Technology, South China Agricultural UniversityNational Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guandong Province Research Center of Woody Forage Engineering and Technology, South China Agricultural UniversityNational Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guandong Province Research Center of Woody Forage Engineering and Technology, South China Agricultural UniversityNational Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guandong Province Research Center of Woody Forage Engineering and Technology, South China Agricultural UniversityNational Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guandong Province Research Center of Woody Forage Engineering and Technology, South China Agricultural UniversityNational Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guandong Province Research Center of Woody Forage Engineering and Technology, South China Agricultural UniversityNational Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guandong Province Research Center of Woody Forage Engineering and Technology, South China Agricultural UniversityNational Engineering Research Center For Breeding Swine Industry, Guandong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, Guandong Province Research Center of Woody Forage Engineering and Technology, South China Agricultural UniversityAbstract Background Milk is a complex liquid that provides nutrition to newborns. Recent reports demonstrated that milk is enriched in maternal-derived exosomes that are involved in fetal physiological and pathological conditions by transmission of exosomal mRNAs, miRNAs and proteins. Until now, there is no such research relevant to exosomal mRNAs and proteins in porcine milk, therefore, we have attempted to investigate porcine milk exosomal mRNAs and proteins using RNA-sequencing and proteomic analysis. Results A total of 16,304 (13,895 known and 2,409 novel mRNAs) mRNAs and 639 (571 known, 66 candidate and 2 putative proteins) proteins were identified. GO and KEGG annotation indicated that most proteins were located in the cytoplasm and participated in many immunity and disease-related pathways, and some mRNAs were closely related to metabolisms, degradation and signaling pathways. Interestingly, 19 categories of proteins were tissue-specific and detected in placenta, liver, milk, plasma and mammary. COG analysis divided the identified mRNAs and proteins into 6 and 23 categories, respectively, 18 mRNAs and 10 proteins appeared to be involved in cell cycle control, cell division and chromosome partitioning. Additionally, 14 selected mRNAs were identified by qPCR, meanwhile, 10 proteins related to immunity and cell proliferation were detected by Western blot. Conclusions These results provide the first insight into porcine milk exosomal mRNA and proteins, and will facilitate further research into the physiological significance of milk exosomes for infants.http://link.springer.com/article/10.1186/s12917-017-1021-8Porcine milk exosomesRNA-seqProteomic analysis |