Cell elasticity determines macrophage function.
Macrophages serve to maintain organ homeostasis in response to challenges from injury, inflammation, malignancy, particulate exposure, or infection. Until now, receptor ligation has been understood as being the central mechanism that regulates macrophage function. Using macrophages of different orig...
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2012-01-01
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doaj-dcc7691e0a0b4faf9febcdc0ef9abf402020-11-24T22:25:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4102410.1371/journal.pone.0041024Cell elasticity determines macrophage function.Naimish R PatelMedhavi BoleCheng ChenCharles C HardinAlvin T KhoJustin MihLinhong DengJames ButlerDaniel TschumperlinJeffrey J FredbergRamaswamy KrishnanHenry KozielMacrophages serve to maintain organ homeostasis in response to challenges from injury, inflammation, malignancy, particulate exposure, or infection. Until now, receptor ligation has been understood as being the central mechanism that regulates macrophage function. Using macrophages of different origins and species, we report that macrophage elasticity is a major determinant of innate macrophage function. Macrophage elasticity is modulated not only by classical biologic activators such as LPS and IFN-γ, but to an equal extent by substrate rigidity and substrate stretch. Macrophage elasticity is dependent upon actin polymerization and small rhoGTPase activation, but functional effects of elasticity are not predicted by examination of gene expression profiles alone. Taken together, these data demonstrate an unanticipated role for cell elasticity as a common pathway by which mechanical and biologic factors determine macrophage function.http://europepmc.org/articles/PMC3445606?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Naimish R Patel Medhavi Bole Cheng Chen Charles C Hardin Alvin T Kho Justin Mih Linhong Deng James Butler Daniel Tschumperlin Jeffrey J Fredberg Ramaswamy Krishnan Henry Koziel |
spellingShingle |
Naimish R Patel Medhavi Bole Cheng Chen Charles C Hardin Alvin T Kho Justin Mih Linhong Deng James Butler Daniel Tschumperlin Jeffrey J Fredberg Ramaswamy Krishnan Henry Koziel Cell elasticity determines macrophage function. PLoS ONE |
author_facet |
Naimish R Patel Medhavi Bole Cheng Chen Charles C Hardin Alvin T Kho Justin Mih Linhong Deng James Butler Daniel Tschumperlin Jeffrey J Fredberg Ramaswamy Krishnan Henry Koziel |
author_sort |
Naimish R Patel |
title |
Cell elasticity determines macrophage function. |
title_short |
Cell elasticity determines macrophage function. |
title_full |
Cell elasticity determines macrophage function. |
title_fullStr |
Cell elasticity determines macrophage function. |
title_full_unstemmed |
Cell elasticity determines macrophage function. |
title_sort |
cell elasticity determines macrophage function. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Macrophages serve to maintain organ homeostasis in response to challenges from injury, inflammation, malignancy, particulate exposure, or infection. Until now, receptor ligation has been understood as being the central mechanism that regulates macrophage function. Using macrophages of different origins and species, we report that macrophage elasticity is a major determinant of innate macrophage function. Macrophage elasticity is modulated not only by classical biologic activators such as LPS and IFN-γ, but to an equal extent by substrate rigidity and substrate stretch. Macrophage elasticity is dependent upon actin polymerization and small rhoGTPase activation, but functional effects of elasticity are not predicted by examination of gene expression profiles alone. Taken together, these data demonstrate an unanticipated role for cell elasticity as a common pathway by which mechanical and biologic factors determine macrophage function. |
url |
http://europepmc.org/articles/PMC3445606?pdf=render |
work_keys_str_mv |
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