The efficacy of Salvadora persica extracts in preserving the viability of human foreskin fibroblasts
Objective: To evaluate the efficacy of Salvadora persica hexane and ethanol extracts in preserving the viability of human foreskin fibroblasts. Materials and methods: Normal human foreskin cells were cultivated in Dulbecco modified Minimum Essential Medium (D-MEM) supplemented with 10% fetal bovine...
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doaj-dcca887b1e0a4ba796ecc20fad9f832e2020-11-24T21:20:55ZengElsevierSaudi Dental Journal1013-90522015-07-01273137140The efficacy of Salvadora persica extracts in preserving the viability of human foreskin fibroblastsHanan Abdul Ghafour Balto0Hassan Suliman Halawany1Vimal Jacob2Nimmi Biju Abraham3Department of Restorative Dental Science, Division of Endodontics, College of Dentistry, King Saud University, Riyadh, Saudi ArabiaDental Caries Research Chair, College of Dentistry, King Saud University, Riyadh, Saudi Arabia; Corresponding author at: Dental Caries Research Chair, College of Dentistry, King Saud University, P.O. Box 60169, Riyadh 11545, Saudi Arabia. Tel.: +966 555415198, +966 4677401; fax: +966 14677425.Dental Caries Research Chair, College of Dentistry, King Saud University, Riyadh, Saudi ArabiaDental Caries Research Chair, College of Dentistry, King Saud University, Riyadh, Saudi ArabiaObjective: To evaluate the efficacy of Salvadora persica hexane and ethanol extracts in preserving the viability of human foreskin fibroblasts. Materials and methods: Normal human foreskin cells were cultivated in Dulbecco modified Minimum Essential Medium (D-MEM) supplemented with 10% fetal bovine serum and 2 mM of l-glutamine. Cell pellets were suspended in the following test solutions: (1) Hank’s Balanced Salt Solution (HBSS); (2) homogenized milk; (3) hexane extract of S. persica; or (4) ethanol extract of S. persica. D-MEM with no serum was used as a positive control. For each condition, cell count was adjusted to 8 × 105 cells/ml, and the cells were incubated in the solutions for either 30, 60, or 120 min. Subsequently, the nonviable cells were separated from the viable cells using the trypan blue dye stain. The ratio of viable to nonviable cells was recorded using a cell counter. Statistical analysis of the data was accomplished by one-way analysis of variance using SPSS Version 16. The level of significance was 5% (p < .05). Results: We did not detect a significant difference when comparing the percentage of viable cells in test solutions at the three incubation periods (30 min, p = 0.478; 60 min, p = 0.606; 120 min, p = 0.091). Homogenized milk preserved the viability of foreskin fibroblasts better than all other tested solutions. Incubation of cells in S. persica hexane and ethanol extracts resulted in a similar percentage of viable cells to incubation of cells in HBSS for each incubation period. Conclusions: S. persica hexane and ethanol extracts should be considered an alternative storage medium to HBSS. Keywords: Avulsion, Cell viability, Fibroblasts, Human foreskin fibroblasts, Storage mediumhttp://www.sciencedirect.com/science/article/pii/S1013905215000152 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hanan Abdul Ghafour Balto Hassan Suliman Halawany Vimal Jacob Nimmi Biju Abraham |
spellingShingle |
Hanan Abdul Ghafour Balto Hassan Suliman Halawany Vimal Jacob Nimmi Biju Abraham The efficacy of Salvadora persica extracts in preserving the viability of human foreskin fibroblasts Saudi Dental Journal |
author_facet |
Hanan Abdul Ghafour Balto Hassan Suliman Halawany Vimal Jacob Nimmi Biju Abraham |
author_sort |
Hanan Abdul Ghafour Balto |
title |
The efficacy of Salvadora persica extracts in preserving the viability of human foreskin fibroblasts |
title_short |
The efficacy of Salvadora persica extracts in preserving the viability of human foreskin fibroblasts |
title_full |
The efficacy of Salvadora persica extracts in preserving the viability of human foreskin fibroblasts |
title_fullStr |
The efficacy of Salvadora persica extracts in preserving the viability of human foreskin fibroblasts |
title_full_unstemmed |
The efficacy of Salvadora persica extracts in preserving the viability of human foreskin fibroblasts |
title_sort |
efficacy of salvadora persica extracts in preserving the viability of human foreskin fibroblasts |
publisher |
Elsevier |
series |
Saudi Dental Journal |
issn |
1013-9052 |
publishDate |
2015-07-01 |
description |
Objective: To evaluate the efficacy of Salvadora persica hexane and ethanol extracts in preserving the viability of human foreskin fibroblasts. Materials and methods: Normal human foreskin cells were cultivated in Dulbecco modified Minimum Essential Medium (D-MEM) supplemented with 10% fetal bovine serum and 2 mM of l-glutamine. Cell pellets were suspended in the following test solutions: (1) Hank’s Balanced Salt Solution (HBSS); (2) homogenized milk; (3) hexane extract of S. persica; or (4) ethanol extract of S. persica. D-MEM with no serum was used as a positive control. For each condition, cell count was adjusted to 8 × 105 cells/ml, and the cells were incubated in the solutions for either 30, 60, or 120 min. Subsequently, the nonviable cells were separated from the viable cells using the trypan blue dye stain. The ratio of viable to nonviable cells was recorded using a cell counter. Statistical analysis of the data was accomplished by one-way analysis of variance using SPSS Version 16. The level of significance was 5% (p < .05). Results: We did not detect a significant difference when comparing the percentage of viable cells in test solutions at the three incubation periods (30 min, p = 0.478; 60 min, p = 0.606; 120 min, p = 0.091). Homogenized milk preserved the viability of foreskin fibroblasts better than all other tested solutions. Incubation of cells in S. persica hexane and ethanol extracts resulted in a similar percentage of viable cells to incubation of cells in HBSS for each incubation period. Conclusions: S. persica hexane and ethanol extracts should be considered an alternative storage medium to HBSS. Keywords: Avulsion, Cell viability, Fibroblasts, Human foreskin fibroblasts, Storage medium |
url |
http://www.sciencedirect.com/science/article/pii/S1013905215000152 |
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