Burkholderia cenocepacia Induces Macropinocytosis to Enter Macrophages
Burkholderia cenocepacia is an opportunistic pathogen that infects individuals with cystic fibrosis, chronic granulomatous disease, and other immunocompromised states. B. cenocepacia survives in macrophages in membrane-bound vacuoles; however, the mechanism by which B. cenocepacia gains entry into m...
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Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2018/4271560 |
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doaj-dccfb5477f344fdeb6a500fca36e6ca22020-11-24T23:30:21ZengHindawi LimitedBioMed Research International2314-61332314-61412018-01-01201810.1155/2018/42715604271560Burkholderia cenocepacia Induces Macropinocytosis to Enter MacrophagesRoberto Rosales-Reyes0Concepción Sánchez-Gómez1Vianney Ortiz-Navarrete2José Ignacio Santos-Preciado3Laboratorio de Infectología, Microbiología e Inmunología Clínicas, Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, MexicoLaboratorio de Investigación en Biología del Desarrollo y Teratogénesis Experimental, Hospital Infantil de México Federico Gómez, Mexico City, MexicoDepartamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Mexico City, MexicoLaboratorio de Infectología, Microbiología e Inmunología Clínicas, Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, MexicoBurkholderia cenocepacia is an opportunistic pathogen that infects individuals with cystic fibrosis, chronic granulomatous disease, and other immunocompromised states. B. cenocepacia survives in macrophages in membrane-bound vacuoles; however, the mechanism by which B. cenocepacia gains entry into macrophages remains unknown. After macrophage internalization, survival of B. cenocepacia within a bacteria-containing membrane vacuole (BcCV) is associated with its ability to arrest the maturation of the BcCV. In this study, we show that B. cenocepacia induces localized membrane ruffling, macropinocytosis, and macropinosomes-like compartments upon contact with the macrophage. The Type 3 Secretion System (T3SS) of B. cenocepacia contributes to macrophage entry and macropinosome-like compartment formation. These data demonstrate the ability of Burkholderia to enter macrophages through the induction of macropinocytosis.http://dx.doi.org/10.1155/2018/4271560 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roberto Rosales-Reyes Concepción Sánchez-Gómez Vianney Ortiz-Navarrete José Ignacio Santos-Preciado |
spellingShingle |
Roberto Rosales-Reyes Concepción Sánchez-Gómez Vianney Ortiz-Navarrete José Ignacio Santos-Preciado Burkholderia cenocepacia Induces Macropinocytosis to Enter Macrophages BioMed Research International |
author_facet |
Roberto Rosales-Reyes Concepción Sánchez-Gómez Vianney Ortiz-Navarrete José Ignacio Santos-Preciado |
author_sort |
Roberto Rosales-Reyes |
title |
Burkholderia cenocepacia Induces Macropinocytosis to Enter Macrophages |
title_short |
Burkholderia cenocepacia Induces Macropinocytosis to Enter Macrophages |
title_full |
Burkholderia cenocepacia Induces Macropinocytosis to Enter Macrophages |
title_fullStr |
Burkholderia cenocepacia Induces Macropinocytosis to Enter Macrophages |
title_full_unstemmed |
Burkholderia cenocepacia Induces Macropinocytosis to Enter Macrophages |
title_sort |
burkholderia cenocepacia induces macropinocytosis to enter macrophages |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2018-01-01 |
description |
Burkholderia cenocepacia is an opportunistic pathogen that infects individuals with cystic fibrosis, chronic granulomatous disease, and other immunocompromised states. B. cenocepacia survives in macrophages in membrane-bound vacuoles; however, the mechanism by which B. cenocepacia gains entry into macrophages remains unknown. After macrophage internalization, survival of B. cenocepacia within a bacteria-containing membrane vacuole (BcCV) is associated with its ability to arrest the maturation of the BcCV. In this study, we show that B. cenocepacia induces localized membrane ruffling, macropinocytosis, and macropinosomes-like compartments upon contact with the macrophage. The Type 3 Secretion System (T3SS) of B. cenocepacia contributes to macrophage entry and macropinosome-like compartment formation. These data demonstrate the ability of Burkholderia to enter macrophages through the induction of macropinocytosis. |
url |
http://dx.doi.org/10.1155/2018/4271560 |
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