| Summary: | Ume Ruqia Tulain,1 Arshad Mahmood,2 Sidra Aslam,1 Alia Erum,1 Nadia Shamshad Malik,3 Ayesha Rashid,4 Rizwana Kausar,5 Mohammed S Alqahtani6 1College of Pharmacy, University of Sargodha, Sargodha, Pakistan; 2College of Pharmacy, Al Ain University, Abu Dhabi Campus, Abu Dhabi, United Arab Emirates; 3Faculty of Pharmacy, Capital University of Science & Technology, Islamabad, Pakistan; 4Department of Pharmacy, The Women University Multan, Multan, Pakistan; 5ILM College of Pharmaceutical Sciences, Sargodha, Pakistan; 6Nanobiotechnology Unit, Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaCorrespondence: Ume Ruqia TulainCollege of Pharmacy, University of Sargodha, Sargodha, PakistanTel +92-331-6668588Email umeruqia_tulain@yahoo.comArshad MahmoodCollege of Pharmacy, Al Ain University, Abu Dhabi Campus, Abu Dhabi, United Arab EmiratesEmail Arshad.mahmood@aau.ac.aeIntroduction: The aim of current study was to prepare Linum usitatissimum mucilage (LUM) based nanoparticles, capable of encapsulating hydrophobic drug ezetimibe as nanocarriers.Methods: Solvent evaporation and nanoprecipitation techniques were used to develop nanoparticles by encapsulating ezetimibe in the articulated matrix of polysaccharide fractions. Developed nanoparticles were characterized to determine the particle size, zeta potential, polydispersibility index (PDI), and entrapment efficiency (EE). Morphology and physicochemical characterization were carried out through SEM, FTIR, PXRD and thermal analysis. Saturation solubility and in vitro release studies were also performed. Safety assessment of ezetimibe loaded nanoparticles was evaluated via oral acute toxicity study.Results: The mean particle size, zeta potential, PDI and EE for emulsion solvent evaporation were 683.6 nm, − 28.3 mV, 0.39, 63.7% and for nanoprecipitation were 637.7 nm, 0.07, − 27.1 mV and 80%, respectively. Thermal analysis confirmed enhanced thermal stability, whereas PXRD confirmed amorphous nature of drug. Saturation solubility (p-value < 0.05) demonstrated improved solubility of drug when enclosed in linseed nanoparticles. Nanoprecipitation surpasses emulsion solvent evaporation in dissolution test by possessing smaller size. Acute oral toxicity study indicated no significant changes in behavioral, clinical or histopathological parameters of control and experimental groups.Conclusion: The in vitro release of ezetimibe was augmented by enhancing aqueous solubility through devised nanoparticles. Thus, linseed mucilage could act as biopolymer in the fabrication of nanoparticle formulation. The acute oral toxicological investigations provided evidence that LUMNs were safe after oral administration.Keywords: linseed mucilage, ezetimibe, nanocarrier, hydrophobic, acute oral toxicity
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