Differences in structure and hibernation mechanism highlight diversification of the microsporidian ribosome.
Assembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, conservation of energy via ribosomal hibernation and recycling is critical. The mechanisms by...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2020-10-01
|
Series: | PLoS Biology |
Online Access: | https://doi.org/10.1371/journal.pbio.3000958 |
id |
doaj-dcf3d113ff344aff869cf9d7bbd3e734 |
---|---|
record_format |
Article |
spelling |
doaj-dcf3d113ff344aff869cf9d7bbd3e7342021-07-02T21:22:13ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852020-10-011810e300095810.1371/journal.pbio.3000958Differences in structure and hibernation mechanism highlight diversification of the microsporidian ribosome.Kai EhrenbolgerNathan JespersenHimanshu SharmaYuliya Y SokolovaYuri S TokarevCharles R VossbrinckJonas BarandunAssembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, conservation of energy via ribosomal hibernation and recycling is critical. The mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. Here, we present the cryo-electron microscopy structure of the ribosome from Paranosema locustae spores, bound by the conserved eukaryotic hibernation and recycling factor Lso2. The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the large subunit tRNA binding sites, providing a reversible ribosome inactivation mechanism. Although microsporidian ribosomes are highly compacted, the P. locustae ribosome retains several rRNA segments absent in other microsporidia, and represents an intermediate state of rRNA reduction. In one case, the near complete reduction of an expansion segment has resulted in a single bound nucleotide, which may act as an architectural co-factor to stabilize a protein-protein interface. The presented structure highlights the reductive evolution in these emerging pathogens and sheds light on a conserved mechanism for eukaryotic ribosome hibernation.https://doi.org/10.1371/journal.pbio.3000958 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kai Ehrenbolger Nathan Jespersen Himanshu Sharma Yuliya Y Sokolova Yuri S Tokarev Charles R Vossbrinck Jonas Barandun |
spellingShingle |
Kai Ehrenbolger Nathan Jespersen Himanshu Sharma Yuliya Y Sokolova Yuri S Tokarev Charles R Vossbrinck Jonas Barandun Differences in structure and hibernation mechanism highlight diversification of the microsporidian ribosome. PLoS Biology |
author_facet |
Kai Ehrenbolger Nathan Jespersen Himanshu Sharma Yuliya Y Sokolova Yuri S Tokarev Charles R Vossbrinck Jonas Barandun |
author_sort |
Kai Ehrenbolger |
title |
Differences in structure and hibernation mechanism highlight diversification of the microsporidian ribosome. |
title_short |
Differences in structure and hibernation mechanism highlight diversification of the microsporidian ribosome. |
title_full |
Differences in structure and hibernation mechanism highlight diversification of the microsporidian ribosome. |
title_fullStr |
Differences in structure and hibernation mechanism highlight diversification of the microsporidian ribosome. |
title_full_unstemmed |
Differences in structure and hibernation mechanism highlight diversification of the microsporidian ribosome. |
title_sort |
differences in structure and hibernation mechanism highlight diversification of the microsporidian ribosome. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Biology |
issn |
1544-9173 1545-7885 |
publishDate |
2020-10-01 |
description |
Assembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, conservation of energy via ribosomal hibernation and recycling is critical. The mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. Here, we present the cryo-electron microscopy structure of the ribosome from Paranosema locustae spores, bound by the conserved eukaryotic hibernation and recycling factor Lso2. The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the large subunit tRNA binding sites, providing a reversible ribosome inactivation mechanism. Although microsporidian ribosomes are highly compacted, the P. locustae ribosome retains several rRNA segments absent in other microsporidia, and represents an intermediate state of rRNA reduction. In one case, the near complete reduction of an expansion segment has resulted in a single bound nucleotide, which may act as an architectural co-factor to stabilize a protein-protein interface. The presented structure highlights the reductive evolution in these emerging pathogens and sheds light on a conserved mechanism for eukaryotic ribosome hibernation. |
url |
https://doi.org/10.1371/journal.pbio.3000958 |
work_keys_str_mv |
AT kaiehrenbolger differencesinstructureandhibernationmechanismhighlightdiversificationofthemicrosporidianribosome AT nathanjespersen differencesinstructureandhibernationmechanismhighlightdiversificationofthemicrosporidianribosome AT himanshusharma differencesinstructureandhibernationmechanismhighlightdiversificationofthemicrosporidianribosome AT yuliyaysokolova differencesinstructureandhibernationmechanismhighlightdiversificationofthemicrosporidianribosome AT yuristokarev differencesinstructureandhibernationmechanismhighlightdiversificationofthemicrosporidianribosome AT charlesrvossbrinck differencesinstructureandhibernationmechanismhighlightdiversificationofthemicrosporidianribosome AT jonasbarandun differencesinstructureandhibernationmechanismhighlightdiversificationofthemicrosporidianribosome |
_version_ |
1721321993875226624 |