A reference database for tumor-related genes co-expressed with interleukin-8 using genome-scale <it>in silico</it> analysis

• Main Authors: Kostich Mitchell, Hedrick Joseph A, Gustafson Eric, Bond Richard W, Qiu Ping, Liu Suxing, Laverty Maureen, Wang Lynn, Benbow Lawrence, Greene Jonathan R, Wang Luquan
• Format: Article
• Language: English
• Published: BMC 2002-10-01
• Series: BMC Genomics
• Online Access: http://www.biomedcentral.com/1471-2164/3/29

<p>Abstract</p> <p>Background</p> <p>The EST database provides a rich resource for gene discovery and <it>in silico</it> expression analysis. We report a novel computational approach to identify co-expressed genes using EST database, and its application to IL-8.</p> <p>Results</p> <p>IL-8 is represented in 53 dbEST cDNA libraries. We calculated the frequency of occurrence of all the genes represented in these cDNA libraries, and ranked the candidates based on a Z-score. Additional analysis suggests that most IL-8 related genes are differentially expressed between non-tumor and tumor tissues. To focus on IL-8's function in tumor tissues, we further analyzed and ranked the genes in 16 IL-8 related tumor libraries.</p> <p>Conclusions</p> <p>This method generated a reference database for genes co-expressed with IL-8 and could facilitate further characterization of functional association among genes.</p>